FastQCFastQC Report
Fri 10 Feb 2017
ERR1631541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98726
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.32919393067682273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3080.3119745558414197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2520.2552519093247979No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC1780.1802969835706906No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1670.16915503514778274No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT1660.16814213074570022No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1540.15598727792070985No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1500.1519356603123797No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1320.13370338107489416No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.13370338107489416No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG1180.11952271944573871No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1170.11850981504365618No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1110.11243238863116098No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT1050.1063549622186658No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT1050.1063549622186658No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1040.10534205781658328No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1030.10432915341450073No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG1010.10230334461033568No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG990.10027753580617062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC754.4929038E-1027.13333337
GGTATCA1250.025.161
TGTACCA400.001918774323.12515
ATTCGTG450.003801450820.55555721
TTCGTGA450.003801450820.55555722
TGCATCA555.0974597E-420.18181814
TAGAGTC609.1555266E-418.55
GACAGGC609.1555266E-418.57
GCTGCAT609.1555266E-418.512
TCGTGAT500.00699052418.523
GCATCAG609.1555266E-418.515
AGAAGCG650.001566143617.07692320
AAGCGTG650.001566143617.07692322
TCTAGAG650.001566143617.0769233
TGGCATT650.001566143617.07692327
ACAGGCT650.001566143617.0769238
TATTCCA650.001566143617.0769235
CTGCATC650.001566143617.07692313
GCGTGGC650.001566143617.07692324
CAGACCT1005.7690977E-616.6500024
Produced by FastQC (version 0.11.5)