##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631541.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	98726
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89864878552762	34.0	33.0	34.0	31.0	34.0
2	33.03241294086664	34.0	33.0	34.0	31.0	34.0
3	33.102050118509815	34.0	33.0	34.0	31.0	34.0
4	36.3694062354395	37.0	37.0	37.0	35.0	37.0
5	36.34353665701031	37.0	37.0	37.0	35.0	37.0
6	36.39321961793246	37.0	37.0	37.0	35.0	37.0
7	36.42608836578004	37.0	37.0	37.0	35.0	37.0
8	36.43381682636793	37.0	37.0	37.0	35.0	37.0
9	38.26029617324717	39.0	39.0	39.0	37.0	39.0
10	38.21760225269939	39.0	39.0	39.0	37.0	39.0
11	38.26733585884164	39.0	39.0	39.0	37.0	39.0
12	38.237050017219374	39.0	39.0	39.0	37.0	39.0
13	38.25392500455807	39.0	39.0	39.0	37.0	39.0
14	39.76192694933452	41.0	40.0	41.0	38.0	41.0
15	39.71537386301481	41.0	40.0	41.0	38.0	41.0
16	39.65468063124202	41.0	40.0	41.0	37.0	41.0
17	39.67426007333428	41.0	40.0	41.0	37.0	41.0
18	39.603184571440146	41.0	40.0	41.0	37.0	41.0
19	39.60970767578956	41.0	40.0	41.0	37.0	41.0
20	39.559447359358224	41.0	40.0	41.0	37.0	41.0
21	39.54626947308713	41.0	40.0	41.0	37.0	41.0
22	39.44962826408444	41.0	40.0	41.0	37.0	41.0
23	39.38035573202601	41.0	39.0	41.0	36.0	41.0
24	39.32818102627474	41.0	39.0	41.0	36.0	41.0
25	39.358720093997526	41.0	39.0	41.0	36.0	41.0
26	39.23172214006442	41.0	39.0	41.0	36.0	41.0
27	39.188086218422704	41.0	39.0	41.0	35.0	41.0
28	39.10677025302352	41.0	39.0	41.0	35.0	41.0
29	39.05875858436481	41.0	39.0	41.0	35.0	41.0
30	39.008619816461724	41.0	39.0	41.0	35.0	41.0
31	38.97584223001033	40.0	39.0	41.0	35.0	41.0
32	38.90772440897028	40.0	38.0	41.0	35.0	41.0
33	38.78684439762575	40.0	38.0	41.0	35.0	41.0
34	38.725381358507384	40.0	38.0	41.0	35.0	41.0
35	38.632356218220124	40.0	38.0	41.0	35.0	41.0
36	38.572949375037986	40.0	38.0	41.0	35.0	41.0
37	38.50040516176083	40.0	38.0	41.0	35.0	41.0
38	38.42784069039564	40.0	38.0	41.0	35.0	41.0
39	38.32707696047647	40.0	38.0	41.0	35.0	41.0
40	38.2155663148512	40.0	37.0	41.0	34.0	41.0
41	38.11031541843081	40.0	37.0	41.0	34.0	41.0
42	37.9714968701254	40.0	37.0	41.0	34.0	41.0
43	37.10987986953791	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	1.0
17	3.0
18	4.0
19	7.0
20	6.0
21	14.0
22	34.0
23	51.0
24	89.0
25	121.0
26	176.0
27	261.0
28	366.0
29	558.0
30	695.0
31	924.0
32	1149.0
33	1502.0
34	2187.0
35	3374.0
36	5043.0
37	9022.0
38	20455.0
39	52682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00664465287766	17.52122034722363	12.24804002998197	26.224094969916738
2	20.669327228896137	19.290764337661813	32.25594068431821	27.78396774912384
3	21.30543119340397	20.985353402345886	27.948058262261206	29.761157141988935
4	15.445779227356521	15.13279176711302	31.95814679010595	37.463282215424506
5	16.91246480157203	34.62613698519134	31.056661872252494	17.404736340984137
6	35.663351092923854	32.40382472702226	15.311062941879545	16.62176123817434
7	30.794319632113123	29.017685310860358	19.627048599153213	20.560946457873307
8	29.515021372282884	30.673784008265297	18.603002248647773	21.208192370804042
9	28.153677855883963	13.662054575289185	18.90586066487045	39.2784069039564
10	20.572088406296213	24.312744363187004	28.840427040495918	26.274740190020868
11	37.83197941778255	20.93875979985009	19.02639628871827	22.202864493649088
12	24.33097664242449	24.602435022182608	25.67712659279217	25.389461742600734
13	31.413204221785545	17.699491521990154	23.987602050118507	26.899702206105786
14	24.385673480136944	19.660474444421936	22.68601989344246	33.26783218199866
15	27.79612260194883	25.061280716325996	20.802017705568947	26.34057897615623
16	27.17116058586391	23.839718007414458	22.14310313392622	26.846018272795412
17	25.746004092133784	25.037983915078094	22.6951360330612	26.52087595972692
18	25.744991187731703	21.968883576768025	24.925551526446935	27.360573709053337
19	26.21700463910216	23.89137613192067	24.043311792233048	25.848307436744122
20	26.251443388772966	22.71438121670077	24.110163482770496	26.92401191175577
21	28.766485019143893	23.164110771225413	23.002046066892206	25.067358142738488
22	28.60138160160444	22.7589490103924	22.895691104673542	25.74397828332962
23	26.650527723193484	22.82478779652776	23.381885217673158	27.142799262605593
24	25.982010817819013	23.56825962765634	23.969369770881023	26.48035978364362
25	26.299049895670844	22.701213459473696	23.970382675283105	27.02935396957235
26	26.52594048173733	24.24386686384539	23.997731094139336	25.23246156027794
27	27.667483742884347	22.517877762696756	23.522678929562627	26.29195956485627
28	25.807791260660817	22.70020055507161	24.90529343840528	26.586714745862285
29	25.377306889775742	23.022304154933856	25.153455016915505	26.446933938374894
30	24.374531531714037	23.924801977189393	25.356035897332006	26.344630593764563
31	25.77233960658793	23.464943378643923	23.86706642627069	26.895650588497453
32	25.52519093247979	22.67082632741122	24.784757814557462	27.019224925551526
33	25.086603326378054	22.76806515001114	24.908332151611532	27.23699937199927
34	26.410469379899926	22.727548973927842	25.18384214897798	25.678139497194252
35	26.074185118408526	22.328464639507324	25.747016996535866	25.850333245548285
36	24.429228369426493	23.209691469319125	25.037983915078094	27.323096246176288
37	24.969106415736483	21.865567327755606	26.206875595081335	26.958450661426575
38	24.54368656686182	21.06638575451249	27.061766910438994	27.3281607681867
39	24.304641127970342	21.318598950631042	27.62494175799688	26.751818163401737
40	23.973421388489356	21.417863582035128	28.176974657131858	26.43174037234366
41	22.17450317039078	22.02358041448048	29.426898689301705	26.37501772582704
42	22.850110406579827	22.281871037011527	28.714826894637685	26.153191661770965
43	21.844296335311874	20.75035958106274	29.463363247776677	27.94198083584871
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	51.0
2	29.0
3	45.5
4	62.0
5	62.0
6	71.0
7	80.0
8	83.5
9	87.0
10	124.5
11	162.0
12	162.0
13	273.0
14	384.0
15	526.0
16	668.0
17	619.0
18	570.0
19	570.0
20	640.5
21	711.0
22	560.5
23	410.0
24	477.0
25	544.0
26	544.0
27	612.5
28	681.0
29	837.0
30	993.0
31	1210.0
32	1427.0
33	1427.0
34	1765.0
35	2103.0
36	2488.5
37	2874.0
38	3299.0
39	3724.0
40	3724.0
41	4259.5
42	4795.0
43	5045.5
44	5296.0
45	5706.0
46	6116.0
47	6116.0
48	6473.0
49	6830.0
50	6942.5
51	7055.0
52	7564.5
53	8074.0
54	8074.0
55	7881.5
56	7689.0
57	7389.0
58	7089.0
59	6658.0
60	6227.0
61	6227.0
62	5989.5
63	5752.0
64	5351.5
65	4951.0
66	4381.0
67	3811.0
68	3811.0
69	3191.5
70	2572.0
71	2315.0
72	2058.0
73	1855.0
74	1652.0
75	1652.0
76	1544.0
77	1436.0
78	1162.0
79	888.0
80	613.0
81	338.0
82	338.0
83	255.0
84	172.0
85	162.5
86	153.0
87	136.0
88	119.0
89	119.0
90	87.5
91	56.0
92	32.5
93	9.0
94	6.5
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	98726.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.77677612786905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.93584716911113	55.20328991349797
2	5.489133065492037	6.891801551769544
3	2.028171741129774	3.8196625002532265
4	1.0665246785097697	2.678119239106213
5	0.7018732755699695	2.203067074529506
6	0.43403197960533746	1.6348277049612059
7	0.3485163851106055	1.5315114559487875
8	0.2646142923987931	1.3289305755322813
9	0.20007422108201428	1.130401312724105
>10	1.403746551139939	16.53870307720357
>50	0.09842360875808767	4.175191945384195
>100	0.029043032092550464	2.8644936490893986
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	325	0.32919393067682273	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	308	0.3119745558414197	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	252	0.2552519093247979	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	178	0.1802969835706906	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	167	0.16915503514778274	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	166	0.16814213074570022	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	154	0.15598727792070985	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	150	0.1519356603123797	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	132	0.13370338107489416	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.13370338107489416	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	118	0.11952271944573871	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	117	0.11850981504365618	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	111	0.11243238863116098	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	105	0.1063549622186658	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	105	0.1063549622186658	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	104	0.10534205781658328	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	103	0.10432915341450073	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	101	0.10230334461033568	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	99	0.10027753580617062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0010129044020825314
9	0.0	0.0	0.0	0.0	0.0010129044020825314
10	0.0	0.0	0.0	0.0	0.0010129044020825314
11	0.0	0.0	0.0	0.0	0.0010129044020825314
12	0.0	0.0	0.0	0.0	0.0010129044020825314
13	0.0	0.0	0.0	0.0	0.0010129044020825314
14	0.0	0.0	0.0	0.0010129044020825314	0.0010129044020825314
15	0.0	0.0	0.0	0.0010129044020825314	0.0010129044020825314
16	0.0	0.0	0.0	0.0010129044020825314	0.0010129044020825314
17	0.0	0.0	0.0	0.0020258088041650627	0.0010129044020825314
18	0.0	0.0	0.0	0.0020258088041650627	0.0010129044020825314
19	0.0	0.0	0.0	0.0020258088041650627	0.0010129044020825314
20	0.0	0.0	0.0	0.0030387132062475943	0.0010129044020825314
21	0.0	0.0	0.0	0.0030387132062475943	0.0010129044020825314
22	0.0	0.0	0.0	0.009116139618742783	0.0010129044020825314
23	0.0	0.0	0.0	0.012154852824990377	0.0010129044020825314
24	0.0	0.0	0.0	0.02329680124789822	0.0010129044020825314
25	0.0	0.0	0.0	0.02836132325831088	0.0010129044020825314
26	0.0	0.0	0.0	0.04254198488746632	0.0010129044020825314
27	0.0	0.0	0.0	0.10534205781658328	0.0010129044020825314
28	0.0	0.0	0.0	0.33020683507890525	0.0010129044020825314
29	0.0	0.0	0.0	0.6411684865182424	0.0010129044020825314
30	0.0	0.0	0.0	1.0402528209387598	0.0010129044020825314
31	0.0	0.0	0.0	1.8617182910276928	0.0010129044020825314
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	75	4.4929038E-10	27.133333	37
GGTATCA	125	0.0	25.16	1
TGTACCA	40	0.0019187743	23.125	15
ATTCGTG	45	0.0038014508	20.555557	21
TTCGTGA	45	0.0038014508	20.555557	22
TGCATCA	55	5.0974597E-4	20.181818	14
TAGAGTC	60	9.1555266E-4	18.5	5
GACAGGC	60	9.1555266E-4	18.5	7
GCTGCAT	60	9.1555266E-4	18.5	12
TCGTGAT	50	0.006990524	18.5	23
GCATCAG	60	9.1555266E-4	18.5	15
AGAAGCG	65	0.0015661436	17.076923	20
AAGCGTG	65	0.0015661436	17.076923	22
TCTAGAG	65	0.0015661436	17.076923	3
TGGCATT	65	0.0015661436	17.076923	27
ACAGGCT	65	0.0015661436	17.076923	8
TATTCCA	65	0.0015661436	17.076923	5
CTGCATC	65	0.0015661436	17.076923	13
GCGTGGC	65	0.0015661436	17.076923	24
CAGACCT	100	5.7690977E-6	16.650002	4
>>END_MODULE