FastQCFastQC Report
Fri 10 Feb 2017
ERR1631540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815954
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43800.5367949663829088No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30150.36950612411974204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29090.356515195709562No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9960.1220657046843327No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA8930.10944244405934649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA405.938049E-527.7500028
CGTATAT400.001930709323.12500210
TCGCTAT400.001930709323.12500211
ATCGCTA400.001930709323.12500210
GTATACT450.003824871520.5555554
CCGTTTA450.003824871520.55555527
GGTATCA19600.019.727041
ATACACA6300.019.38095337
TAGAACG500.007033175318.54
CGGCTAG500.007033175318.521
GGCAGTC6850.017.8248188
CCTTAGG752.0663408E-417.2666661
TCGGTGA6750.017.26666613
TATACAC11050.017.24434537
GGGTATC1401.8662831E-917.1785721
CTATCCC650.001579402717.07692314
GTATAGA650.001579402717.0769231
GGACCGT650.001579402717.0769236
ATACCGT1301.391345E-817.0769236
AGTCGGT6950.017.0359711
Produced by FastQC (version 0.11.5)