##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631540.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	815954
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.047811518786595	34.0	33.0	34.0	31.0	34.0
2	33.18831331177983	34.0	33.0	34.0	31.0	34.0
3	33.268668331793215	34.0	34.0	34.0	31.0	34.0
4	36.50766587332129	37.0	37.0	37.0	35.0	37.0
5	36.50439975782948	37.0	37.0	37.0	35.0	37.0
6	36.56176206011613	37.0	37.0	37.0	35.0	37.0
7	36.56131473097748	37.0	37.0	37.0	35.0	37.0
8	36.5630268372972	37.0	37.0	37.0	35.0	37.0
9	38.40773989710204	39.0	39.0	39.0	37.0	39.0
10	38.370855464891406	39.0	39.0	39.0	37.0	39.0
11	38.42980364089152	39.0	39.0	39.0	37.0	39.0
12	38.38590900957652	39.0	39.0	39.0	37.0	39.0
13	38.41275733681066	39.0	39.0	39.0	37.0	39.0
14	39.94473217852967	41.0	40.0	41.0	38.0	41.0
15	39.935319883228715	41.0	40.0	41.0	38.0	41.0
16	39.91816327881229	41.0	40.0	41.0	38.0	41.0
17	39.90423602310915	41.0	40.0	41.0	38.0	41.0
18	39.90939440213541	41.0	40.0	41.0	38.0	41.0
19	39.93729303367592	41.0	40.0	41.0	38.0	41.0
20	39.91948810839827	41.0	40.0	41.0	38.0	41.0
21	39.88835522590734	41.0	40.0	41.0	38.0	41.0
22	39.83887817205382	41.0	40.0	41.0	38.0	41.0
23	39.784533196724325	41.0	40.0	41.0	38.0	41.0
24	39.76116668341598	41.0	40.0	41.0	38.0	41.0
25	39.73479632430259	41.0	40.0	41.0	37.0	41.0
26	39.647318353730725	41.0	40.0	41.0	37.0	41.0
27	39.59026856906149	41.0	40.0	41.0	37.0	41.0
28	39.523835902514115	41.0	40.0	41.0	37.0	41.0
29	39.452460555374444	41.0	39.0	41.0	36.0	41.0
30	39.367178787039464	41.0	39.0	41.0	36.0	41.0
31	39.288836135370374	41.0	39.0	41.0	35.0	41.0
32	39.211677373969614	41.0	39.0	41.0	35.0	41.0
33	39.13627729014136	41.0	39.0	41.0	35.0	41.0
34	39.06962034624501	41.0	39.0	41.0	35.0	41.0
35	38.987299529140124	41.0	39.0	41.0	35.0	41.0
36	38.922938057782666	41.0	39.0	41.0	35.0	41.0
37	38.85169752216424	41.0	39.0	41.0	35.0	41.0
38	38.77430100226238	41.0	38.0	41.0	35.0	41.0
39	38.6762746429333	40.0	38.0	41.0	35.0	41.0
40	38.57212171274361	40.0	38.0	41.0	35.0	41.0
41	38.47110008652449	40.0	38.0	41.0	35.0	41.0
42	38.33183488284879	40.0	38.0	41.0	35.0	41.0
43	37.52317777717862	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	1.0
12	0.0
13	3.0
14	6.0
15	0.0
16	5.0
17	6.0
18	10.0
19	26.0
20	39.0
21	89.0
22	152.0
23	234.0
24	423.0
25	622.0
26	986.0
27	1458.0
28	2139.0
29	3199.0
30	4193.0
31	5416.0
32	6750.0
33	8943.0
34	13567.0
35	20225.0
36	33623.0
37	69748.0
38	154772.0
39	489313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.181912215639606	17.90787225750471	14.20888922659856	25.701326300257122
2	18.625927441988154	19.89965120582778	35.066192456927716	26.408228895256354
3	19.205616983310332	21.890939930437256	29.409623581721515	29.493819504530894
4	14.426425999504874	16.497866301286592	33.362052272554585	35.71365542665395
5	16.017692173823527	35.234094078832875	32.119825382313216	16.628388365030382
6	33.566353005193925	34.11209455434987	16.511224897481966	15.810327542974237
7	30.129639661059326	29.973625964208768	20.232145439571347	19.66458893516056
8	28.643894141091288	32.21836034874515	19.063329550440343	20.07441595972322
9	27.74519151814931	14.607563661677005	19.12080828085897	38.526436539314716
10	18.92006167014317	25.690418822629706	29.91357355929378	25.475945947933337
11	36.586376192775575	21.795836530000464	20.147459293048385	21.470327984175576
12	23.6243709816975	25.609777021743874	27.02762655737946	23.73822543917917
13	31.35581172468056	18.677033264130085	24.337278817188224	25.629876194001135
14	23.898406037595258	19.90724967338845	24.388752307115354	31.80559198190094
15	26.815972469036243	26.128433710723886	21.46603852668165	25.589555293558213
16	25.682330131355446	25.166492228728583	23.552675763584713	25.598501876331266
17	24.125747284773404	26.168141831524817	24.51106312365648	25.1950477600453
18	24.978491434566163	23.922304443632854	25.931731445645216	25.16747267615576
19	25.37741098150141	25.136466026271087	25.631346865141907	23.854776127085593
20	26.123899141373165	23.575838834051922	25.572642575439303	24.727619449135613
21	27.54211634479395	23.70207144030178	24.856548285810227	23.89926392909404
22	26.884358677082286	23.505124063366317	24.311909740009856	25.298607519541545
23	25.347997558685908	23.850241557734872	24.92211570750312	25.879645176076103
24	24.727129225422022	24.545746451393093	25.55732308438956	25.169801238795326
25	24.927875836137822	23.704890226654936	25.343340433406787	26.023893503800462
26	25.316133017302445	24.358358436872667	25.510506719741556	24.815001826083332
27	25.49359400162264	23.82916193805043	24.846131031896405	25.831113028430526
28	24.524421719851855	24.074151238917878	25.916411954595482	25.48501508663479
29	24.25063177581089	24.464736982722066	26.355284734188444	24.929346507278595
30	24.094005299318344	24.41743039436047	26.5350742811482	24.953490025172986
31	24.900423308176688	24.119742044281907	25.498496238758555	25.48133840878285
32	24.079543699767388	23.679030925762973	26.63177090865416	25.609654465815474
33	24.0435122568184	23.114170651776938	27.212931121117123	25.629385970287537
34	24.76671479029455	22.78155386210497	26.67527826323543	25.77645308436505
35	24.070474561065943	22.821139426977503	27.34762008642644	25.760765925530116
36	23.011346227851078	22.945901362086587	27.577044784387354	26.465707625674977
37	23.214176289349645	22.075141490819338	28.230267882748294	26.480414337082724
38	22.58803805116465	21.522414253744696	29.583775555974967	26.305772139115685
39	22.783637312887738	21.489446709005655	29.925338928419986	25.801577049686625
40	22.127595428173645	21.97967042259735	29.93685918568939	25.95587496353961
41	20.881936971937144	22.423690551183032	30.73089904577954	25.963473431100283
42	20.960495322040213	22.659243045563844	30.2964382796089	26.083823352787043
43	19.911294019025583	22.681425668603865	30.409189733734994	26.99809057863556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	71.0
2	92.0
3	273.5
4	455.0
5	455.0
6	607.0
7	759.0
8	781.0
9	803.0
10	1201.0
11	1599.0
12	1599.0
13	2510.0
14	3421.0
15	5801.0
16	8181.0
17	7451.0
18	6721.0
19	6721.0
20	7355.5
21	7990.0
22	6652.0
23	5314.0
24	5771.0
25	6228.0
26	6228.0
27	7364.5
28	8501.0
29	10218.0
30	11935.0
31	14109.0
32	16283.0
33	16283.0
34	19441.0
35	22599.0
36	25931.5
37	29264.0
38	33067.5
39	36871.0
40	36871.0
41	39892.0
42	42913.0
43	46400.5
44	49888.0
45	53838.0
46	57788.0
47	57788.0
48	61325.0
49	64862.0
50	64758.5
51	64655.0
52	63068.5
53	61482.0
54	61482.0
55	57922.5
56	54363.0
57	52371.5
58	50380.0
59	48507.5
60	46635.0
61	46635.0
62	43395.5
63	40156.0
64	36876.5
65	33597.0
66	30180.5
67	26764.0
68	26764.0
69	23039.5
70	19315.0
71	16510.5
72	13706.0
73	10889.5
74	8073.0
75	8073.0
76	6598.5
77	5124.0
78	4208.5
79	3293.0
80	2863.5
81	2434.0
82	2434.0
83	1933.5
84	1433.0
85	1219.0
86	1005.0
87	820.5
88	636.0
89	636.0
90	466.5
91	297.0
92	179.5
93	62.0
94	37.0
95	12.0
96	12.0
97	12.0
98	12.0
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	815954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.32309322109879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.38062587718628	61.75083927188252
2	5.53517912076135	7.563611181265147
3	1.4627710393664142	2.9982312625126526
4	0.680128874262212	1.8587403391431236
5	0.3981571337673022	1.3601663483514446
6	0.2581480929054119	1.0582485729855198
7	0.19707000125855798	0.9425102446949359
8	0.13746100003420061	0.7513408575681723
9	0.11076427917699008	0.6810982354599574
>10	0.7153871897467767	9.408700477426756
>50	0.0739039910643267	3.5612317731977123
>100	0.04860968515696275	6.215969061009197
>500	0.0012556007193042463	0.5865347265984342
>1k	5.381145939875341E-4	1.2627776479043598
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4380	0.5367949663829088	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3015	0.36950612411974204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2909	0.356515195709562	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	996	0.1220657046843327	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	893	0.10944244405934649	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.225559283979244E-4	0.0
3	0.0	0.0	0.0	2.451118567958488E-4	0.0
4	0.0	0.0	0.0	2.451118567958488E-4	0.0
5	0.0	0.0	0.0	2.451118567958488E-4	0.0
6	0.0	0.0	0.0	2.451118567958488E-4	0.0
7	0.0	0.0	0.0	2.451118567958488E-4	0.0
8	0.0	0.0	0.0	2.451118567958488E-4	0.0
9	0.0	0.0	0.0	8.578914987854708E-4	0.0
10	0.0	0.0	0.0	9.804474271833952E-4	0.0
11	0.0	0.0	0.0	0.0017157829975709415	0.0
12	0.0	0.0	0.0	0.002818786353152261	0.0
13	0.0	0.0	0.0	0.0031864541383460343	0.0
14	0.0	0.0	0.0	0.003676677851937732	0.0
15	0.0	0.0	0.0	0.004657125279121127	0.0
16	0.0	0.0	0.0	0.007108243847079615	0.0
17	0.0	0.0	0.0	0.008946582773048481	0.0
18	0.0	0.0	0.0	0.00919169462984433	0.0
19	0.0	0.0	0.0	0.010172142057027725	0.0
20	0.0	0.0	0.0	0.011275145412609044	0.0
21	0.0	0.0	0.0	0.012745816553384137	0.0
22	0.0	0.0	0.0	0.017893165546096962	0.0
23	0.0	0.0	0.0	0.02536907717837035	0.0
24	0.0	0.0	0.0	0.04313968679606939	0.0
25	0.0	0.0	0.0	0.050247930643149	0.0
26	0.0	0.0	0.0	0.06470953019410408	0.0
27	0.0	0.0	0.0	0.1533174664258034	1.225559283979244E-4
28	0.0	0.0	0.0	0.536549854526113	1.225559283979244E-4
29	0.0	0.0	0.0	1.0907477627415272	1.225559283979244E-4
30	0.0	0.0	0.0	1.8920184226071568	1.225559283979244E-4
31	0.0	0.0	0.0	3.292219904553443	1.225559283979244E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	40	5.938049E-5	27.750002	8
CGTATAT	40	0.0019307093	23.125002	10
TCGCTAT	40	0.0019307093	23.125002	11
ATCGCTA	40	0.0019307093	23.125002	10
GTATACT	45	0.0038248715	20.555555	4
CCGTTTA	45	0.0038248715	20.555555	27
GGTATCA	1960	0.0	19.72704	1
ATACACA	630	0.0	19.380953	37
TAGAACG	50	0.0070331753	18.5	4
CGGCTAG	50	0.0070331753	18.5	21
GGCAGTC	685	0.0	17.824818	8
CCTTAGG	75	2.0663408E-4	17.266666	1
TCGGTGA	675	0.0	17.266666	13
TATACAC	1105	0.0	17.244345	37
GGGTATC	140	1.8662831E-9	17.178572	1
CTATCCC	65	0.0015794027	17.076923	14
GTATAGA	65	0.0015794027	17.076923	1
GGACCGT	65	0.0015794027	17.076923	6
ATACCGT	130	1.391345E-8	17.076923	6
AGTCGGT	695	0.0	17.03597	11
>>END_MODULE