FastQCFastQC Report
Fri 10 Feb 2017
ERR1631539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154997
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4280.27613437679438957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.2309722123654006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3030.19548765459976644No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.12580888662361206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1750.025.371431
TAGACCT400.00192369723.1255
CTGTCCC502.687511E-422.1999999
CTAGAAC450.003811111720.5555553
ACACGCT450.003811111720.5555559
AGCGCGC450.003811111720.55555530
TAGAACT555.115545E-420.1818184
CCTCTAG555.115545E-420.18181826
GCCTGAC609.187743E-418.57
ATACACA701.21058125E-418.537
ACTGATC500.00700811818.4999988
CGCGCGT500.00700811818.49999832
TAGGACC500.00700811818.4999984
TATACAC1054.7314097E-717.6190537
CAGTGTA752.0527614E-417.26666624
GTGCATG650.001571609217.0769231
GGTCCAA650.001571609217.07692311
GCACACG650.001571609217.0769237
ACTGTAC650.001571609217.0769238
GTATCAA2650.016.7547152
Produced by FastQC (version 0.11.5)