FastQCFastQC Report
Fri 10 Feb 2017
ERR1631538.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631538.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39151
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4021.026793696201885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3520.8990830374703073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2800.7151796888968354No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1580.40356568159178563No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC1330.33971035222599677No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.3294934995274706No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA1110.2835176623841026No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1100.28096344920947103No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1090.27840923603483947No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1050.26819238333631323No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC920.23498761206610305No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG910.2324333988914715No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT810.20689126714515593No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC750.1915659880973666No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT740.18901177492273505No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC700.17879492222420884No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC680.17368649587494572No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG670.17113228270031416No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC640.16346964317641952No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT630.16091543000178796No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC630.16091543000178796No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG610.15580700365252484No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC600.15325279047789328No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG580.14814436412863016No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT580.14814436412863016No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG570.14559015095399863No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT570.14559015095399863No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC560.14303593777936707No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC550.14048172460473551No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG540.13792751143010396No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG530.1353732982554724No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC530.1353732982554724No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA520.13281908508084084No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC510.13026487190620928No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG510.13026487190620928No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA510.13026487190620928No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.12771065873157775No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG500.12771065873157775No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC490.1251564455569462No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC490.1251564455569462No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG490.1251564455569462No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC460.11749380603305153No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT450.11493959285841997No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC450.11493959285841997No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.11493959285841997No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA450.11493959285841997No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA440.11238537968378841No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC440.11238537968378841No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.11238537968378841No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCA440.11238537968378841No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG430.10983116650915685No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC420.1072769533345253No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC420.1072769533345253No Hit
GGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA410.10472274015989375No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT410.10472274015989375No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT410.10472274015989375No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT400.10216852698526219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG358.7166677E-426.4285719
ACATCTA751.2896635E-824.66666624
CCGGACA802.5596819E-823.12520
CATCTAA802.5596819E-823.12525
GGACATC802.5596819E-823.12522
CGGACAT802.5596819E-823.12521
GACATCT802.5596819E-823.12523
CATCACA652.574714E-622.7692335
TCACAGA652.574714E-622.7692337
GGTATCA2300.022.5217381
CCCGGAC854.8665243E-821.76470619
ATCTAAG854.8665243E-821.76470626
CCCCGGA854.8665243E-821.76470618
GGCATCA704.897556E-621.14285733
GCATCAC704.897556E-621.14285734
CAGCCCC908.906136E-820.55555515
TCTAAGG908.906136E-820.55555527
ATCACAG758.896903E-619.73333436
CTAAGGG851.1837219E-619.58823628
CTTATAC851.1837219E-619.58823637
Produced by FastQC (version 0.11.5)