##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631538.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39151
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35082117953564	33.0	31.0	34.0	31.0	34.0
2	32.53061224489796	34.0	31.0	34.0	31.0	34.0
3	32.58815866772241	34.0	31.0	34.0	31.0	34.0
4	36.10068708334398	37.0	35.0	37.0	35.0	37.0
5	35.996015427447574	37.0	35.0	37.0	35.0	37.0
6	36.03391995095911	37.0	35.0	37.0	35.0	37.0
7	36.077724706904036	37.0	35.0	37.0	35.0	37.0
8	36.085923731194605	37.0	35.0	37.0	35.0	37.0
9	37.80120558861842	39.0	37.0	39.0	35.0	39.0
10	37.71793823912544	39.0	37.0	39.0	35.0	39.0
11	37.793951623202474	39.0	37.0	39.0	35.0	39.0
12	37.63247426630227	39.0	37.0	39.0	35.0	39.0
13	37.75964854026717	39.0	37.0	39.0	35.0	39.0
14	39.037904523511536	40.0	38.0	41.0	36.0	41.0
15	38.99348675640469	40.0	38.0	41.0	36.0	41.0
16	38.92247963014993	40.0	38.0	41.0	36.0	41.0
17	38.97060100635999	40.0	38.0	41.0	36.0	41.0
18	38.96263186125514	40.0	38.0	41.0	36.0	41.0
19	38.97570943270925	40.0	38.0	41.0	36.0	41.0
20	38.932262266608774	40.0	38.0	41.0	35.0	41.0
21	38.91323337845777	40.0	38.0	41.0	35.0	41.0
22	38.823733748818675	40.0	38.0	41.0	35.0	41.0
23	38.711016321422186	40.0	38.0	41.0	35.0	41.0
24	38.670991800975706	40.0	38.0	41.0	35.0	41.0
25	38.68263901305203	40.0	38.0	41.0	35.0	41.0
26	38.53927102755996	40.0	38.0	41.0	35.0	41.0
27	38.41480421956017	40.0	38.0	41.0	35.0	41.0
28	38.260606370207654	40.0	38.0	41.0	34.0	41.0
29	38.133840770350695	40.0	38.0	41.0	34.0	41.0
30	38.03994789405124	40.0	37.0	41.0	34.0	41.0
31	37.93846900462312	40.0	37.0	41.0	34.0	41.0
32	37.81576460371382	40.0	37.0	41.0	33.0	41.0
33	37.63822124594519	40.0	36.0	41.0	33.0	41.0
34	37.52220888355342	40.0	36.0	41.0	33.0	41.0
35	37.348931061786416	40.0	36.0	41.0	33.0	41.0
36	37.24216495108682	40.0	36.0	41.0	33.0	41.0
37	37.109243697478995	39.0	35.0	41.0	32.0	41.0
38	36.94480345329621	39.0	35.0	41.0	32.0	41.0
39	36.75420806620521	39.0	35.0	41.0	31.0	41.0
40	36.562514367449104	39.0	35.0	41.0	31.0	41.0
41	36.36629971137391	39.0	35.0	41.0	30.0	41.0
42	36.13787642716661	39.0	35.0	41.0	30.0	41.0
43	35.1556282087303	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	3.0
20	10.0
21	11.0
22	29.0
23	48.0
24	56.0
25	95.0
26	143.0
27	196.0
28	284.0
29	397.0
30	478.0
31	676.0
32	770.0
33	1015.0
34	1449.0
35	1894.0
36	3264.0
37	6056.0
38	11104.0
39	11170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70003320477127	18.934382263543714	14.101810937140813	23.2637735945442
2	21.087583969758118	20.862813210390538	31.965977880513908	26.083624939337437
3	21.45794488007969	22.862762126127045	29.69017394191719	25.98911905187607
4	14.794002707465964	16.60749406145437	32.4589410232178	36.13956220786187
5	14.70460524635386	37.31450026819238	30.635232816530866	17.345661668922887
6	32.063037981149904	32.99277157671579	17.20262573114352	17.74156471099078
7	27.861357308881	32.67860335623611	18.832213736558455	20.62782559832444
8	31.383617276697912	28.241935071901104	19.317514239738447	21.056933411662538
9	28.528006947459833	13.27424586856019	20.58951240070496	37.608234783275016
10	21.9279201042119	26.43610635743659	27.674899747132898	23.961073791218617
11	35.728333886746185	23.26632780771883	17.406962785114047	23.598375520420934
12	23.963628004393247	28.49735638936426	24.39529003090598	23.143725575336518
13	32.379760414804224	18.9088401317974	25.478276416949758	23.23312303644862
14	23.37871318740262	21.835968429925163	25.245843018058288	29.53947536461393
15	28.418175780950676	26.68131082220122	21.795101019131057	23.105412377717045
16	25.003192766468292	25.802661490127964	24.277796224872926	24.91634951853082
17	24.88825317360987	26.413118438864906	23.473219074863987	25.225409312661235
18	24.57408495313019	21.856402135322213	27.429695282368264	26.13981762917933
19	24.119435008045773	25.769456718857757	26.750274577916272	23.3608336951802
20	26.293070419657226	22.088835534213686	27.84603202983321	23.77206201629588
21	27.672345533958264	23.909989527725983	26.553600163469643	21.86406477484611
22	28.625067048095833	22.41066639421726	25.59577022298281	23.368496334704094
23	25.491047482822914	22.196112487548213	26.02487803632091	26.287961993307963
24	22.663533498505785	24.995530126944395	27.32497254220837	25.01596383234145
25	23.49365278026104	23.664785062961354	26.93928635283901	25.902275803938597
26	24.06324231820388	24.980204847896605	26.16535976092565	24.791193072973872
27	25.797553063778704	23.17948455978136	26.865214170774692	24.157748205665243
28	23.56261653597609	23.989170136139563	27.17682817807974	25.271385149804605
29	22.722280401522312	23.927869019948407	28.704247656509413	24.645602922019872
30	20.944548031978748	25.690276110444177	28.854946233812672	24.5102296237644
31	24.4131695231284	25.26883093662997	25.881842098541547	24.436157441700086
32	23.03389440882736	23.925314806773773	27.411815790145845	25.628974994253017
33	21.956016449132846	23.828254706137773	27.82815253761079	26.387576307118593
34	23.70309826058083	23.2152435442262	28.106561773645627	24.975096421547345
35	22.92150902914357	24.321217848841663	28.20873030063089	24.548542821383872
36	21.925365891037266	24.809072565196292	28.257260350948894	25.00830119281755
37	23.128400296288728	22.60478659548926	29.47562003524814	24.791193072973872
38	22.155245076754106	21.703149344844324	28.808970396669302	27.332635181732268
39	22.530714413424942	22.231871471993053	30.361932006845294	24.875482107736712
40	21.16165615182243	22.71717197517305	29.874077290490664	26.247094582513856
41	20.538428137212332	22.75037674644326	31.32231616050676	25.38887895583765
42	20.43625961022707	23.348062629307044	31.03369007177339	25.181987688692498
43	18.53337079512656	22.213991979770633	30.619907537483076	28.63272968761973
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	36.0
1	25.5
2	15.0
3	38.5
4	62.0
5	62.0
6	72.5
7	83.0
8	92.5
9	102.0
10	156.0
11	210.0
12	210.0
13	318.0
14	426.0
15	633.0
16	840.0
17	747.0
18	654.0
19	654.0
20	707.5
21	761.0
22	563.0
23	365.0
24	363.5
25	362.0
26	362.0
27	368.5
28	375.0
29	437.0
30	499.0
31	591.5
32	684.0
33	684.0
34	742.0
35	800.0
36	897.0
37	994.0
38	1120.0
39	1246.0
40	1246.0
41	1368.5
42	1491.0
43	1732.5
44	1974.0
45	2193.5
46	2413.0
47	2413.0
48	2775.5
49	3138.0
50	3027.0
51	2916.0
52	3098.0
53	3280.0
54	3280.0
55	2994.5
56	2709.0
57	2550.0
58	2391.0
59	2352.5
60	2314.0
61	2314.0
62	2236.5
63	2159.0
64	1984.5
65	1810.0
66	1695.5
67	1581.0
68	1581.0
69	1292.0
70	1003.0
71	838.5
72	674.0
73	486.0
74	298.0
75	298.0
76	233.0
77	168.0
78	122.0
79	76.0
80	70.5
81	65.0
82	65.0
83	66.0
84	67.0
85	58.0
86	49.0
87	38.5
88	28.0
89	28.0
90	27.5
91	27.0
92	15.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	39151.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.02135322213992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.16018352284063	57.08155602666598
2	5.162577299022542	6.610303695946464
3	1.8192698982645121	3.4941636228959667
4	0.8777179333732297	2.2477075936757682
5	0.5705166566925992	1.8262624198615616
6	0.39497307001795334	1.5172026257311435
7	0.2952323957709954	1.3230824244591453
8	0.23139836425294236	1.1851549130290413
9	0.13165769000598446	0.7586013128655718
>10	1.2128465988430082	14.954918137467754
>50	0.10373030121683623	4.176138540522592
>100	0.03989626969878316	4.824908686879007
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	402	1.026793696201885	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	352	0.8990830374703073	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	280	0.7151796888968354	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	158	0.40356568159178563	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	133	0.33971035222599677	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129	0.3294934995274706	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	111	0.2835176623841026	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	110	0.28096344920947103	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	109	0.27840923603483947	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	105	0.26819238333631323	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	92	0.23498761206610305	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	91	0.2324333988914715	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	81	0.20689126714515593	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	75	0.1915659880973666	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	74	0.18901177492273505	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	70	0.17879492222420884	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	68	0.17368649587494572	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	67	0.17113228270031416	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	64	0.16346964317641952	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	63	0.16091543000178796	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	63	0.16091543000178796	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	61	0.15580700365252484	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	60	0.15325279047789328	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG	58	0.14814436412863016	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	58	0.14814436412863016	No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG	57	0.14559015095399863	No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT	57	0.14559015095399863	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	56	0.14303593777936707	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	55	0.14048172460473551	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	54	0.13792751143010396	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	53	0.1353732982554724	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	53	0.1353732982554724	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	52	0.13281908508084084	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	51	0.13026487190620928	No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG	51	0.13026487190620928	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	51	0.13026487190620928	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.12771065873157775	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	50	0.12771065873157775	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	49	0.1251564455569462	No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC	49	0.1251564455569462	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	49	0.1251564455569462	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	46	0.11749380603305153	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	45	0.11493959285841997	No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC	45	0.11493959285841997	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.11493959285841997	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	45	0.11493959285841997	No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA	44	0.11238537968378841	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC	44	0.11238537968378841	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44	0.11238537968378841	No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCA	44	0.11238537968378841	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	43	0.10983116650915685	No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC	42	0.1072769533345253	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	42	0.1072769533345253	No Hit
GGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAA	41	0.10472274015989375	No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT	41	0.10472274015989375	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	41	0.10472274015989375	No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT	40	0.10216852698526219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.002554213174631555	0.0
14	0.0	0.0	0.0	0.002554213174631555	0.0
15	0.0	0.0	0.0	0.007662639523894664	0.0
16	0.0	0.0	0.0	0.012771065873157773	0.0
17	0.0	0.0	0.0	0.017879492222420884	0.0
18	0.0	0.0	0.0	0.017879492222420884	0.0
19	0.0	0.0	0.0	0.017879492222420884	0.0
20	0.0	0.0	0.0	0.02043370539705244	0.0
21	0.0	0.0	0.0	0.03320477127021021	0.0
22	0.0	0.0	0.0	0.05108426349263109	0.0
23	0.0	0.0	0.0	0.07151796888968354	0.0
24	0.0	0.0	0.0	0.10472274015989375	0.0
25	0.0	0.0	0.0	0.11749380603305151	0.0
26	0.0	0.0	0.0	0.1583612168271564	0.0
27	0.0	0.0	0.0	0.2503128911138924	0.0
28	0.0	0.0	0.0	0.5363847666726265	0.0
29	0.0	0.0	0.0	0.9220709560419913	0.0
30	0.0	0.0	0.0	1.514648412556512	0.0
31	0.0	0.0	0.0	2.554213174631555	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAG	35	8.7166677E-4	26.42857	19
ACATCTA	75	1.2896635E-8	24.666666	24
CCGGACA	80	2.5596819E-8	23.125	20
CATCTAA	80	2.5596819E-8	23.125	25
GGACATC	80	2.5596819E-8	23.125	22
CGGACAT	80	2.5596819E-8	23.125	21
GACATCT	80	2.5596819E-8	23.125	23
CATCACA	65	2.574714E-6	22.76923	35
TCACAGA	65	2.574714E-6	22.76923	37
GGTATCA	230	0.0	22.521738	1
CCCGGAC	85	4.8665243E-8	21.764706	19
ATCTAAG	85	4.8665243E-8	21.764706	26
CCCCGGA	85	4.8665243E-8	21.764706	18
GGCATCA	70	4.897556E-6	21.142857	33
GCATCAC	70	4.897556E-6	21.142857	34
CAGCCCC	90	8.906136E-8	20.555555	15
TCTAAGG	90	8.906136E-8	20.555555	27
ATCACAG	75	8.896903E-6	19.733334	36
CTAAGGG	85	1.1837219E-6	19.588236	28
CTTATAC	85	1.1837219E-6	19.588236	37
>>END_MODULE