FastQCFastQC Report
Fri 10 Feb 2017
ERR1631537.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631537.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423578
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.14613601272965074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6080.14353908843235483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4810.11355641699993863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTC250.005492472629.63
ATACGAC250.005492472629.67
TACACAT405.931411E-527.7537
TCGTTTA551.898983E-523.54545430
AATCTAA400.001929185123.12521
TATGGTC603.719139E-521.58333235
AGCCTTA603.719139E-521.5833327
GGTATCA3700.021.51
TAACGCC705.0893777E-621.1428594
GTAGACC1001.2840246E-820.354
ATAACGC656.8889865E-519.9230773
CGTTTAT656.8889865E-519.92307731
ATACCGC1052.2493623E-819.38095327
AGCACCG609.2236936E-418.55
GCGTTAT801.6127458E-518.51
ATCGTTT701.21689154E-418.529
TACTGGA500.00702772618.52
AACGCCG701.21689154E-418.55
GGTATCC1002.8642899E-718.526
GTGTAGA1002.8642899E-718.514
Produced by FastQC (version 0.11.5)