##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631537.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423578
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77862872953742	34.0	31.0	34.0	31.0	34.0
2	32.95674468456813	34.0	33.0	34.0	31.0	34.0
3	33.081385246636984	34.0	33.0	34.0	31.0	34.0
4	36.416761493750855	37.0	37.0	37.0	35.0	37.0
5	36.38400483500087	37.0	37.0	37.0	35.0	37.0
6	36.41916482914599	37.0	37.0	37.0	35.0	37.0
7	36.42132971967383	37.0	37.0	37.0	35.0	37.0
8	36.402863227079784	37.0	37.0	37.0	35.0	37.0
9	38.250383636543916	39.0	39.0	39.0	37.0	39.0
10	38.20514757612529	39.0	39.0	39.0	37.0	39.0
11	38.26516013579553	39.0	39.0	39.0	37.0	39.0
12	38.05871409752159	39.0	38.0	39.0	37.0	39.0
13	38.17787751016342	39.0	39.0	39.0	37.0	39.0
14	39.69875205983313	41.0	40.0	41.0	37.0	41.0
15	39.68177289660936	41.0	40.0	41.0	37.0	41.0
16	39.660822800050994	41.0	40.0	41.0	37.0	41.0
17	39.65139360401154	41.0	40.0	41.0	37.0	41.0
18	39.63756144086803	41.0	40.0	41.0	37.0	41.0
19	39.63695942659911	41.0	40.0	41.0	37.0	41.0
20	39.598244007006976	41.0	40.0	41.0	37.0	41.0
21	39.56901916530131	41.0	40.0	41.0	37.0	41.0
22	39.517925860172156	41.0	39.0	41.0	37.0	41.0
23	39.467151268479476	41.0	39.0	41.0	37.0	41.0
24	39.427035870607064	41.0	39.0	41.0	37.0	41.0
25	39.427274315474364	41.0	39.0	41.0	37.0	41.0
26	39.3520414185817	41.0	39.0	41.0	36.0	41.0
27	39.29721326414497	41.0	39.0	41.0	36.0	41.0
28	39.22314898318609	41.0	39.0	41.0	36.0	41.0
29	39.165313118245045	41.0	39.0	41.0	36.0	41.0
30	39.11823796325588	40.0	39.0	41.0	35.0	41.0
31	39.076772164748874	40.0	39.0	41.0	35.0	41.0
32	39.007328048198914	40.0	39.0	41.0	35.0	41.0
33	38.93184726307788	40.0	39.0	41.0	35.0	41.0
34	38.89108735581168	40.0	39.0	41.0	35.0	41.0
35	38.80835406938037	40.0	38.0	41.0	35.0	41.0
36	38.75629045890013	40.0	38.0	41.0	35.0	41.0
37	38.70628077945502	40.0	38.0	41.0	35.0	41.0
38	38.62906241589506	40.0	38.0	41.0	35.0	41.0
39	38.574489232207526	40.0	38.0	41.0	35.0	41.0
40	38.500911284344326	40.0	38.0	41.0	35.0	41.0
41	38.42871914971977	40.0	38.0	41.0	35.0	41.0
42	38.318824395979014	40.0	38.0	41.0	34.0	41.0
43	37.4301144063195	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	9.0
19	20.0
20	26.0
21	52.0
22	98.0
23	179.0
24	276.0
25	412.0
26	623.0
27	946.0
28	1289.0
29	1893.0
30	2504.0
31	3268.0
32	4443.0
33	6127.0
34	8944.0
35	13142.0
36	20880.0
37	39053.0
38	94713.0
39	224678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57568145654401	17.845591602963324	11.76855266326391	26.81017427722875
2	19.530995471908362	19.802492102989298	33.09567541279292	27.570837012309426
3	20.785545991529304	20.748716883313108	28.411768316579238	30.05396880857835
4	15.416286964856532	14.917205331721666	32.53592018471214	37.13058751870966
5	16.932654670450304	34.074243704819416	31.983719645496222	17.009381979234046
6	36.237009476412844	32.86195222603629	15.108433393613454	15.79260490393741
7	31.20912795282097	28.879686858146552	19.685866593638007	20.225318595394473
8	28.600871622227785	31.11587476214534	19.50667881712459	20.776574798502285
9	27.453975418931105	13.695234407830434	19.02601173809782	39.82477843514064
10	18.800787576314164	25.005311890608105	30.811562451307665	25.38233808177006
11	38.159913876546945	20.41347756493491	19.973653022583797	21.45295553593435
12	23.87801066155466	24.1872807369599	26.89020676239087	25.04450183909457
13	31.457488349253264	18.17256798039558	23.859360023419534	26.510583646931618
14	24.43091945285166	19.303174385827404	22.97947485469028	33.286431306630654
15	27.04342529593133	26.213589940931776	20.693945389042867	26.04903937409403
16	27.038231447336734	24.06829438733834	22.41452577801491	26.478948387310012
17	25.45221895377003	24.672433412500176	23.59612633328454	26.279221300445254
18	26.39301380147222	22.865446269636287	24.590984423175897	26.150555505715595
19	26.102630448229135	23.734471573122306	24.083403765068063	26.079494213580496
20	26.25986241022905	23.666479373338557	24.003371279905945	26.07028693652645
21	27.71886169725529	23.47832040379812	23.873761149068176	24.929056749878416
22	27.274787642417692	23.1463862617983	23.436297446987332	26.14252864879668
23	26.26340367063445	23.133165556284794	23.98967840633838	26.61375236674237
24	26.024722719310255	23.67261755804126	23.51491342798729	26.787746294661197
25	26.611627610499127	23.442907799744084	23.661757692798023	26.283706896958765
26	26.479420555364065	23.643107054662895	23.749817034879054	26.12765535509399
27	26.568660317580235	23.047230970447004	23.77814711812228	26.605961593850484
28	25.7539343403104	23.072255877311854	24.48876003947325	26.685049742904493
29	25.314581966013343	23.36641657498737	24.52228397131107	26.796717487688216
30	24.978398311527037	24.256689440905806	24.747272049067703	26.017640198499446
31	26.294802846229032	23.64287097063587	23.739665421716897	26.322660761418206
32	25.65359862882397	23.26962212390634	24.03240961523025	27.044369632039434
33	25.39225361090519	22.889762924420058	24.747980301148782	26.970003163525963
34	26.188801118093952	22.5153336575554	24.671016908338014	26.624848316012635
35	25.589619857499677	22.753542440825537	25.583245588769955	26.073592112904826
36	24.96989928655407	22.497391271501353	25.715688727932047	26.81702071401253
37	25.490936734202435	21.957231017663805	25.8434101865536	26.708422061580155
38	24.573750289202934	21.475147434474877	27.025718993904313	26.92538328241788
39	24.811722988446046	21.173668131961527	27.271246382012286	26.743362497580137
40	23.925699635014094	22.124142424771826	27.48490242647163	26.465255513742452
41	23.609583122825075	21.411404747177613	28.600163370146703	26.378848759850605
42	23.642398802581816	21.844146768717923	28.422628181822475	26.09082624687779
43	22.520291422122963	21.08206752947509	28.739925114146626	27.65771593425532
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	75.0
2	66.0
3	132.5
4	199.0
5	199.0
6	220.0
7	241.0
8	231.5
9	222.0
10	299.0
11	376.0
12	376.0
13	587.5
14	799.0
15	1050.5
16	1302.0
17	1257.5
18	1213.0
19	1213.0
20	1440.0
21	1667.0
22	1558.0
23	1449.0
24	1762.0
25	2075.0
26	2075.0
27	2455.5
28	2836.0
29	3506.0
30	4176.0
31	4930.5
32	5685.0
33	5685.0
34	7397.5
35	9110.0
36	11268.5
37	13427.0
38	15330.0
39	17233.0
40	17233.0
41	18960.5
42	20688.0
43	22784.0
44	24880.0
45	26823.0
46	28766.0
47	28766.0
48	30418.5
49	32071.0
50	33353.5
51	34636.0
52	35515.0
53	36394.0
54	36394.0
55	35056.0
56	33718.0
57	32364.0
58	31010.0
59	30087.5
60	29165.0
61	29165.0
62	27699.5
63	26234.0
64	23177.5
65	20121.0
66	17610.5
67	15100.0
68	15100.0
69	12509.5
70	9919.0
71	8538.0
72	7157.0
73	5580.0
74	4003.0
75	4003.0
76	3145.0
77	2287.0
78	1955.0
79	1623.0
80	1480.5
81	1338.0
82	1338.0
83	1116.0
84	894.0
85	758.0
86	622.0
87	550.0
88	478.0
89	478.0
90	360.0
91	242.0
92	148.0
93	54.0
94	35.5
95	17.0
96	17.0
97	9.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	423578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.7934699532215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.38193282082032	51.3748704175532
2	6.253077017288263	7.352801914622348
3	2.0057053560539453	3.5376713275851923
4	1.0040010063324365	2.3611481199524103
5	0.6384976930356964	1.8769747465347728
6	0.3922576874920661	1.3837314338090962
7	0.3256417311672062	1.340192513582592
8	0.22340143044663352	1.050763623077668
9	0.18197803267931437	0.9629207996829867
>10	1.3674474111857997	15.980527901113007
>50	0.16400417842116147	6.663425843474512
>100	0.061249717738503455	5.82427863662613
>500	8.05917338664519E-4	0.29069272238611543
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.14613601272965074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.14353908843235483	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	481	0.11355641699993863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.3608402702689942E-4	0.0
4	0.0	0.0	0.0	2.3608402702689942E-4	0.0
5	0.0	0.0	0.0	2.3608402702689942E-4	0.0
6	0.0	0.0	0.0	2.3608402702689942E-4	0.0
7	0.0	0.0	0.0	2.3608402702689942E-4	0.0
8	0.0	0.0	0.0	2.3608402702689942E-4	0.0
9	0.0	0.0	0.0	4.7216805405379884E-4	0.0
10	0.0	0.0	0.0	4.7216805405379884E-4	0.0
11	0.0	0.0	0.0	7.082520810806983E-4	0.0
12	0.0	0.0	0.0	7.082520810806983E-4	0.0
13	0.0	0.0	0.0	7.082520810806983E-4	0.0
14	0.0	0.0	0.0	7.082520810806983E-4	0.0
15	0.0	0.0	0.0	0.001180420135134497	0.0
16	0.0	0.0	0.0	0.0014165041621613966	0.0
17	0.0	0.0	0.0	0.0016525881891882959	0.0
18	0.0	0.0	0.0	0.0018886722162151954	0.0
19	0.0	0.0	0.0	0.0021247562432420946	0.0
20	0.0	0.0	0.0	0.0035412604054034912	0.0
21	0.0	0.0	0.0	0.004249512486484189	0.0
22	0.0	0.0	0.0	0.004721680540537988	0.0
23	0.0	0.0	0.0	0.005666016648645586	0.0
24	0.0	0.0	0.0	0.00826294094594148	0.0
25	0.0	0.0	0.0	0.012276369405398769	0.0
26	0.0	0.0	0.0	0.02006714229728645	0.0
27	0.0	0.0	0.0	0.07129737616212363	0.0
28	0.0	2.3608402702689942E-4	0.0	0.23348710272960352	0.0
29	0.0	2.3608402702689942E-4	0.0	0.45068440759435097	0.0
30	0.0	2.3608402702689942E-4	0.0	0.7292635594860923	0.0
31	0.0	2.3608402702689942E-4	0.0	1.3874658268370879	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGTC	25	0.0054924726	29.6	3
ATACGAC	25	0.0054924726	29.6	7
TACACAT	40	5.931411E-5	27.75	37
TCGTTTA	55	1.898983E-5	23.545454	30
AATCTAA	40	0.0019291851	23.125	21
TATGGTC	60	3.719139E-5	21.583332	35
AGCCTTA	60	3.719139E-5	21.583332	7
GGTATCA	370	0.0	21.5	1
TAACGCC	70	5.0893777E-6	21.142859	4
GTAGACC	100	1.2840246E-8	20.35	4
ATAACGC	65	6.8889865E-5	19.923077	3
CGTTTAT	65	6.8889865E-5	19.923077	31
ATACCGC	105	2.2493623E-8	19.380953	27
AGCACCG	60	9.2236936E-4	18.5	5
GCGTTAT	80	1.6127458E-5	18.5	1
ATCGTTT	70	1.21689154E-4	18.5	29
TACTGGA	50	0.007027726	18.5	2
AACGCCG	70	1.21689154E-4	18.5	5
GGTATCC	100	2.8642899E-7	18.5	26
GTGTAGA	100	2.8642899E-7	18.5	14
>>END_MODULE