FastQCFastQC Report
Fri 10 Feb 2017
ERR1631536.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631536.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65881
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2370.3597395303653557No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1670.2534873484009047No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1640.24893368345957106No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1640.24893368345957106No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1580.2398263535769038No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1380.20946858730134638No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1310.19884336910490127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1230.18670026259467828No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1190.1806287093395668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.1639319378880102No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1080.1639319378880102No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1050.15937827294667659No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC980.14875305475023146No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT970.1472351664364536No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT940.14268150149512No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT920.13964572486756424No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC910.13812783655378638No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT910.13812783655378638No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG900.1366099482400085No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC900.1366099482400085No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT900.1366099482400085No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT850.12902050667111914No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC850.12902050667111914No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC850.12902050667111914No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC840.12750261835734125No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG820.12446684172978553No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG790.1199131767884519No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC790.1199131767884519No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT790.1199131767884519No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC770.11687740016089616No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA740.11232373521956256No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT730.11080584690578467No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC720.10928795859200681No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG720.10928795859200681No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT720.10928795859200681No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG710.10777007027822892No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG710.10777007027822892No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC700.10625218196445105No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG700.10625218196445105No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC690.10473429365067319No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC670.10169851702311744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA308.193518E-637.025
CGCTTAT308.193518E-637.026
TCCGCTT308.193518E-637.024
CTCCGCT308.193518E-637.023
CCTCCGC352.3545377E-531.71428722
TCAAAGA250.00545926229.5999984
TCCTCCG405.8568097E-527.75000221
GCTTATT405.8568097E-527.75000227
AAGAATG358.780542E-426.42857233
GCCAAGA358.780542E-426.42857230
TTGCCAA358.780542E-426.42857228
GGTATCA1500.023.4333341
AGAATGT400.001912024123.12500234
TGCCAAG400.001912024123.12500229
ATGTTTT400.001912024123.12500237
GGTGATT1450.022.96551715
TCGGTGA1450.022.96551713
TTATTGA1450.022.96551729
CTTATTG1450.022.96551728
CGGTGAT1450.022.96551714
Produced by FastQC (version 0.11.5)