##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631536.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65881
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76040132967016	34.0	31.0	34.0	31.0	34.0
2	32.943048830467056	34.0	33.0	34.0	31.0	34.0
3	33.02964435876808	34.0	33.0	34.0	31.0	34.0
4	36.40400115359512	37.0	37.0	37.0	35.0	37.0
5	36.374584478074105	37.0	37.0	37.0	35.0	37.0
6	36.42851504986263	37.0	37.0	37.0	35.0	37.0
7	36.42286850533538	37.0	37.0	37.0	35.0	37.0
8	36.40292345289234	37.0	37.0	37.0	35.0	37.0
9	38.23100742247385	39.0	39.0	39.0	37.0	39.0
10	38.18531898422914	39.0	39.0	39.0	37.0	39.0
11	38.240585297733794	39.0	39.0	39.0	37.0	39.0
12	38.04697864331143	39.0	39.0	39.0	37.0	39.0
13	38.14151272749351	39.0	39.0	39.0	37.0	39.0
14	39.622895827325024	41.0	40.0	41.0	37.0	41.0
15	39.62505122873059	41.0	40.0	41.0	37.0	41.0
16	39.58130568752751	41.0	39.0	41.0	37.0	41.0
17	39.54296382872148	41.0	39.0	41.0	37.0	41.0
18	39.53806104946798	41.0	39.0	41.0	37.0	41.0
19	39.53177699184894	41.0	39.0	41.0	37.0	41.0
20	39.51835885915514	41.0	39.0	41.0	37.0	41.0
21	39.49697181281401	41.0	39.0	41.0	37.0	41.0
22	39.415582641429246	41.0	39.0	41.0	36.0	41.0
23	39.315477907135595	41.0	39.0	41.0	36.0	41.0
24	39.26998679437167	41.0	39.0	41.0	36.0	41.0
25	39.24488092166178	41.0	39.0	41.0	36.0	41.0
26	39.17844295016773	41.0	39.0	41.0	36.0	41.0
27	39.100848499567405	40.0	39.0	41.0	35.0	41.0
28	38.912797316373464	40.0	39.0	41.0	35.0	41.0
29	38.80873089358085	40.0	38.0	41.0	35.0	41.0
30	38.72822209741807	40.0	38.0	41.0	35.0	41.0
31	38.685827476814254	40.0	38.0	41.0	35.0	41.0
32	38.579317253836464	40.0	38.0	41.0	35.0	41.0
33	38.44515110578163	40.0	38.0	41.0	34.0	41.0
34	38.3935884397626	40.0	38.0	41.0	34.0	41.0
35	38.23502982650537	40.0	38.0	41.0	34.0	41.0
36	38.16543464731865	40.0	37.0	41.0	34.0	41.0
37	38.05936461195185	40.0	37.0	41.0	34.0	41.0
38	37.92261805376361	40.0	37.0	41.0	33.0	41.0
39	37.815956041954436	40.0	36.0	41.0	33.0	41.0
40	37.68743643842686	40.0	36.0	41.0	33.0	41.0
41	37.5815030130083	40.0	36.0	41.0	33.0	41.0
42	37.436074133665244	39.0	35.0	41.0	33.0	41.0
43	36.43567948270366	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	0.0
19	3.0
20	6.0
21	12.0
22	33.0
23	53.0
24	60.0
25	117.0
26	115.0
27	210.0
28	275.0
29	357.0
30	487.0
31	607.0
32	818.0
33	1104.0
34	1744.0
35	2504.0
36	4164.0
37	7376.0
38	15472.0
39	30361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.240372793369865	16.643645360574368	12.603026669297673	26.512955176758098
2	23.161457779936555	18.486361773500708	29.95552587240631	28.396654574156432
3	21.318741367010215	19.735583855739893	28.11280945947997	30.83286531776992
4	15.109060275344941	15.751127032072981	32.019853979144216	37.11995871343787
5	17.02918899227395	34.62151454895949	30.43517857956012	17.914117879206447
6	34.30579377969369	32.72263626842337	16.575340386454364	16.396229565428573
7	32.19289324691489	28.560586512044445	18.264750079689136	20.98177016135153
8	32.06083696361622	27.542083453499494	17.473930268210864	22.923149314673427
9	28.876307281310243	13.651887494118183	17.581700338489096	39.89010488608248
10	21.681516674003127	22.070096082330263	26.61465369378121	29.6337335498854
11	39.63661753768158	21.00757426268575	17.188567265220627	22.167240934412046
12	26.736084758883443	24.198175498246837	22.467782820540062	26.597956922329658
13	32.620937751400255	16.28845949515035	23.747362669054812	27.343240084394587
14	25.398825154445138	19.430488304670543	19.749244850563898	35.42144169032043
15	31.05295912326771	24.002367905769493	18.038584720936235	26.90608825002656
16	29.984365750368084	22.413138841244063	20.12568115238081	27.476814256007042
17	27.759141482369724	23.72004067940681	20.611405412789725	27.90941242543374
18	28.859610509858687	19.31057512788209	22.64841152987963	29.181402832379593
19	28.070308586694193	23.908258830315265	20.899804192407522	27.12162839058302
20	29.02050667111914	19.436559857925655	22.226438578649383	29.316494892305823
21	32.409951275785126	20.304791973406598	21.059182465354198	26.226074285454075
22	31.890833472473094	19.82362137793901	19.98755331582702	28.29799183376087
23	28.3784399143911	20.08166239128125	21.309634037127548	30.2302636572001
24	26.661708231508324	21.86062749502892	22.651447306507187	28.82621696695557
25	27.06394863465946	19.970856544375465	21.604104370000453	31.361090450964618
26	29.135866182966257	21.613211699883124	21.242846951321322	28.0080751658293
27	29.535070809489838	19.896480017000346	21.12900532778798	29.43944384572183
28	26.912159803281675	20.362471729330156	22.70001973254808	30.02534873484009
29	25.451951245427363	21.285347824107102	24.639880997556197	28.622819932909337
30	24.80381293544421	23.07797392267877	25.180249237261123	26.9379639046159
31	27.772802477193725	22.795646696316084	21.069807683550646	28.361743142939545
32	26.191162854237188	21.226150179869766	23.18270821632944	29.399978749563605
33	25.86481686677494	20.33059607474082	24.073708656517052	29.730878401967182
34	27.756105705742172	21.03944991727509	22.6711798545863	28.53326452239644
35	26.81653283951367	20.878553756014632	24.52755726233664	27.777356142135062
36	24.53211092727797	22.070096082330263	24.563986581867304	28.83380640852446
37	26.48411529879631	20.209165009638593	25.60829374174648	27.69842594981861
38	24.189068168364173	18.043138385877565	27.458599596241704	30.309193849516554
39	25.527845661116256	17.780543707593996	28.296473945447094	28.395136685842655
40	23.95531336804238	18.622971721740715	27.880572547471953	29.54114236274495
41	21.956254458796924	19.190661951093638	30.925456504910372	27.92762708519907
42	23.09770647075788	18.674579924409162	30.653754496744128	27.573959108088825
43	20.227379669403923	18.829404532414504	31.335286349630394	29.607929448551175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	7.0
4	11.0
5	11.0
6	12.0
7	13.0
8	15.5
9	18.0
10	23.0
11	28.0
12	28.0
13	61.0
14	94.0
15	142.0
16	190.0
17	173.0
18	156.0
19	156.0
20	204.0
21	252.0
22	178.5
23	105.0
24	88.5
25	72.0
26	72.0
27	78.0
28	84.0
29	133.0
30	182.0
31	257.5
32	333.0
33	333.0
34	480.0
35	627.0
36	911.5
37	1196.0
38	1491.5
39	1787.0
40	1787.0
41	2036.0
42	2285.0
43	2716.0
44	3147.0
45	3576.5
46	4006.0
47	4006.0
48	4945.5
49	5885.0
50	5878.0
51	5871.0
52	5850.0
53	5829.0
54	5829.0
55	5299.0
56	4769.0
57	4625.0
58	4481.0
59	4612.5
60	4744.0
61	4744.0
62	4641.0
63	4538.0
64	4382.5
65	4227.0
66	3875.5
67	3524.0
68	3524.0
69	2967.5
70	2411.0
71	2124.0
72	1837.0
73	1352.0
74	867.0
75	867.0
76	740.0
77	613.0
78	567.0
79	521.0
80	477.5
81	434.0
82	434.0
83	382.5
84	331.0
85	252.5
86	174.0
87	151.5
88	129.0
89	129.0
90	104.0
91	79.0
92	49.0
93	19.0
94	11.5
95	4.0
96	4.0
97	4.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	65881.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.930283389748176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.06042270081157	43.964117120262294
2	7.3814917840039005	7.814089039328486
3	2.758739353617619	4.380625673562939
4	1.5399615726534943	3.26042409799487
5	1.009434773880875	2.671483432249055
6	0.6423675833787388	2.04004189371746
7	0.4932465372372459	1.827537529788558
8	0.3928766023343179	1.663605591900548
9	0.338390066244157	1.6119973892321005
>10	2.1651228814774455	21.5600856089009
>50	0.18353359525106824	6.5921889467372985
>100	0.034412549109575294	2.613803676325496
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	237	0.3597395303653557	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	167	0.2534873484009047	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	164	0.24893368345957106	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	164	0.24893368345957106	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	158	0.2398263535769038	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	138	0.20946858730134638	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	131	0.19884336910490127	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.18670026259467828	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	119	0.1806287093395668	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	108	0.1639319378880102	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	108	0.1639319378880102	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	105	0.15937827294667659	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	98	0.14875305475023146	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	97	0.1472351664364536	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	94	0.14268150149512	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	92	0.13964572486756424	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	91	0.13812783655378638	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	91	0.13812783655378638	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	90	0.1366099482400085	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	90	0.1366099482400085	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	90	0.1366099482400085	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	85	0.12902050667111914	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	85	0.12902050667111914	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	85	0.12902050667111914	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	84	0.12750261835734125	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	82	0.12446684172978553	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	79	0.1199131767884519	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	79	0.1199131767884519	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	79	0.1199131767884519	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	77	0.11687740016089616	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	74	0.11232373521956256	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	73	0.11080584690578467	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC	72	0.10928795859200681	No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG	72	0.10928795859200681	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	72	0.10928795859200681	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	71	0.10777007027822892	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	71	0.10777007027822892	No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC	70	0.10625218196445105	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	70	0.10625218196445105	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	69	0.10473429365067319	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	67	0.10169851702311744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0030357766275557444	0.0
18	0.0	0.0	0.0	0.0030357766275557444	0.0
19	0.0	0.0	0.0	0.0030357766275557444	0.0
20	0.0	0.0	0.0	0.0030357766275557444	0.0
21	0.0	0.0	0.0	0.004553664941333617	0.0
22	0.0	0.0	0.0	0.004553664941333617	0.0
23	0.0	0.0	0.0	0.004553664941333617	0.0
24	0.0	0.0	0.0	0.010625218196445105	0.0
25	0.0	0.0	0.0	0.01366099482400085	0.0
26	0.0	0.0	0.0	0.022768324706668085	0.0
27	0.0	0.0	0.0	0.08500174557156084	0.0
28	0.0	0.0	0.0	0.2990239978142408	0.0
29	0.0	0.0	0.0	0.6299236502178169	0.0
30	0.0	0.0	0.0	1.0610039313307327	0.0
31	0.0	0.0	0.0	2.0810248781894627	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTTA	30	8.193518E-6	37.0	25
CGCTTAT	30	8.193518E-6	37.0	26
TCCGCTT	30	8.193518E-6	37.0	24
CTCCGCT	30	8.193518E-6	37.0	23
CCTCCGC	35	2.3545377E-5	31.714287	22
TCAAAGA	25	0.005459262	29.599998	4
TCCTCCG	40	5.8568097E-5	27.750002	21
GCTTATT	40	5.8568097E-5	27.750002	27
AAGAATG	35	8.780542E-4	26.428572	33
GCCAAGA	35	8.780542E-4	26.428572	30
TTGCCAA	35	8.780542E-4	26.428572	28
GGTATCA	150	0.0	23.433334	1
AGAATGT	40	0.0019120241	23.125002	34
TGCCAAG	40	0.0019120241	23.125002	29
ATGTTTT	40	0.0019120241	23.125002	37
GGTGATT	145	0.0	22.965517	15
TCGGTGA	145	0.0	22.965517	13
TTATTGA	145	0.0	22.965517	29
CTTATTG	145	0.0	22.965517	28
CGGTGAT	145	0.0	22.965517	14
>>END_MODULE