FastQCFastQC Report
Fri 10 Feb 2017
ERR1631535.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631535.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553061
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.32401489166656117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16450.29743554508453857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12260.22167536673169866No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA7340.13271592102860263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.1310886140950094No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5550.10035059423824859No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC250.00549390929.59999825
TTACGGC405.9346414E-527.75000218
TAGCGCC358.8635524E-426.42857229
ACCGTTA358.8635524E-426.4285728
CCGTTAT400.001929926923.1250029
TCTATAC400.001929926923.1250023
GGTATCA8250.023.0969681
AATACTA502.699647E-422.1999995
GCCCCTA450.00382333520.55555727
AGATAAC1001.2862074E-820.3499981
TATACAC3750.018.74666637
TTCTATA609.2285575E-418.52
CTAAGCC701.2177462E-418.53
CCGATTT500.007030381418.49999820
TTAGCAA500.007030381418.4999984
TTGACCG500.007030381418.49999833
ATTGCCG500.007030381418.49999811
CAGTCGG1653.6379788E-1217.93939410
GCATTTG1900.017.52631624
GTATCAA10900.017.4816512
Produced by FastQC (version 0.11.5)