##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631535.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553061
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44800663941229	34.0	31.0	34.0	31.0	34.0
2	32.64457989263391	34.0	31.0	34.0	31.0	34.0
3	32.75485705916707	34.0	31.0	34.0	31.0	34.0
4	36.235382715469	37.0	37.0	37.0	35.0	37.0
5	36.083896713020806	37.0	35.0	37.0	35.0	37.0
6	36.11224259168519	37.0	36.0	37.0	35.0	37.0
7	36.125280936460896	37.0	35.0	37.0	35.0	37.0
8	36.10798989623206	37.0	35.0	37.0	35.0	37.0
9	37.87086777046293	39.0	38.0	39.0	35.0	39.0
10	37.77949991049812	39.0	37.0	39.0	35.0	39.0
11	37.848987724681365	39.0	38.0	39.0	35.0	39.0
12	37.634671401527136	39.0	37.0	39.0	35.0	39.0
13	37.78313603743529	39.0	38.0	39.0	35.0	39.0
14	39.155581391564404	40.0	38.0	41.0	36.0	41.0
15	39.11658026872262	40.0	38.0	41.0	36.0	41.0
16	39.085650588271456	40.0	38.0	41.0	36.0	41.0
17	39.08169080806638	40.0	38.0	41.0	36.0	41.0
18	39.05362699593716	40.0	38.0	41.0	36.0	41.0
19	39.0511064783089	40.0	39.0	41.0	36.0	41.0
20	39.023778570537424	40.0	39.0	41.0	36.0	41.0
21	38.998540848116214	40.0	38.0	41.0	36.0	41.0
22	38.94416890722723	40.0	38.0	41.0	35.0	41.0
23	38.87456537343982	40.0	38.0	41.0	35.0	41.0
24	38.829991628409886	40.0	38.0	41.0	35.0	41.0
25	38.827641073950254	40.0	38.0	41.0	35.0	41.0
26	38.72516774822307	40.0	38.0	41.0	35.0	41.0
27	38.65236926848937	40.0	38.0	41.0	35.0	41.0
28	38.56403362377748	40.0	38.0	41.0	35.0	41.0
29	38.48114041669906	40.0	38.0	41.0	35.0	41.0
30	38.41494699499694	40.0	38.0	41.0	34.0	41.0
31	38.34357150477072	40.0	38.0	41.0	34.0	41.0
32	38.271102102661374	40.0	38.0	41.0	34.0	41.0
33	38.15614190839708	40.0	38.0	41.0	34.0	41.0
34	38.11647358971253	40.0	38.0	41.0	34.0	41.0
35	38.00110295247721	40.0	37.0	41.0	34.0	41.0
36	37.950918614764014	40.0	37.0	41.0	33.0	41.0
37	37.873171313833375	40.0	37.0	41.0	33.0	41.0
38	37.77540090514428	40.0	37.0	41.0	33.0	41.0
39	37.675251012094506	40.0	37.0	41.0	33.0	41.0
40	37.56038664812742	40.0	37.0	41.0	33.0	41.0
41	37.455832177644055	40.0	36.0	41.0	33.0	41.0
42	37.327687180980035	40.0	36.0	41.0	33.0	41.0
43	36.386272038708206	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	4.0
16	5.0
17	11.0
18	22.0
19	52.0
20	67.0
21	122.0
22	223.0
23	360.0
24	574.0
25	909.0
26	1312.0
27	1883.0
28	2678.0
29	3714.0
30	4913.0
31	6553.0
32	8476.0
33	11753.0
34	16873.0
35	25068.0
36	41650.0
37	79760.0
38	168476.0
39	177600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36140136440646	18.604276924245248	12.618680398726362	25.415641312621933
2	20.33934773921864	20.144251719068965	32.28631199813402	27.230088543578372
3	21.378111998495644	21.594724632545052	28.43284917938528	28.59431418957403
4	15.767700127110754	15.597917770372527	32.659146097808375	35.97523600470834
5	16.55911373248159	34.632526972612425	32.062286076942684	16.746073217963296
6	35.14693677550939	32.838873108029674	15.647279414024853	16.366910702436076
7	30.286894212392486	29.545565498200016	20.008461996054685	20.159078293352813
8	29.13096385389677	30.11168749920895	19.652443401360788	21.104905245533494
9	27.215985216820567	14.314695847293518	19.903048669134147	38.566270266751765
10	19.871406589869835	25.123268500219687	30.176056529026635	24.829268380883846
11	36.68528426339952	21.417348176783392	20.029255362428376	21.868112197388715
12	23.63464427974491	25.202283292439713	26.296195175577374	24.866877252237998
13	31.339942610308807	18.518572092409336	24.202574399568945	25.93891089771291
14	24.006574320011715	20.50460979891911	23.666286359009224	31.822529522059956
15	27.162645711775014	26.516966482901523	20.901311066952832	25.419076738370634
16	26.400342819327342	24.845179826456757	23.21100204136614	25.543475312849758
17	25.309143114412336	25.2369991736897	23.777847289901114	25.676010421996846
18	25.0937961635335	23.07448907082582	25.94306957098765	25.88864519465303
19	25.84416547180148	24.435279291072774	25.36429073827299	24.35626449885275
20	26.56488163150177	23.096909744133107	24.753508202531005	25.58470042183412
21	27.297350563500228	23.56358521031134	24.49729776643083	24.6417664597576
22	27.20821030591562	23.51368113101448	24.35246744934103	24.925641113728865
23	25.891538184757195	23.359448596086146	24.541777489282378	26.207235729874284
24	25.011888381209307	24.316124261157448	25.117482520011354	25.554504837621888
25	25.603685669392707	23.79647091369668	24.507061608032387	26.092781808878225
26	25.436796302758648	24.61446386564954	24.865069133422896	25.08367069816892
27	26.273774502270093	23.167064754159124	24.622600400317506	25.936560343253273
28	25.09506184670407	23.985961765519537	25.24603976776522	25.67293662001117
29	24.94281824247235	24.16984744901557	25.617608184269002	25.269726124243075
30	24.83776653931483	24.445223944555845	25.191434579549092	25.52557493658023
31	25.027619014900708	24.590235073527154	24.84897687596847	25.53316903560367
32	24.547201845727688	23.65453358671105	25.102113510082972	26.69615105747829
33	23.901341804972688	23.79918309191934	25.696442164607518	26.603032938500455
34	25.86821345204236	22.82062918918528	25.44710258000474	25.86405477876762
35	24.9437223018799	22.793507406958728	26.24122836359823	26.021541927563145
36	23.645854616398555	23.687983784790468	26.49074876008252	26.175412838728455
37	24.60922032108574	22.210389089087823	27.10116967206149	26.07922091776495
38	23.94817208228387	21.937182336125673	27.60039127691159	26.514254304678868
39	24.066423052791645	21.57158071171173	28.235221792894453	26.126774442602173
40	22.84467716942616	21.771197028899163	28.89391947723669	26.49020632443799
41	21.908794870728546	22.104794950285772	29.7878534194239	26.198556759561786
42	22.46189841626873	22.28614926744066	29.620059993382284	25.63189232290832
43	21.56308255328074	21.861783781535852	29.733067419326257	26.84206624585715
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	370.0
1	278.5
2	187.0
3	331.5
4	476.0
5	476.0
6	517.0
7	558.0
8	523.5
9	489.0
10	711.5
11	934.0
12	934.0
13	1623.5
14	2313.0
15	3136.5
16	3960.0
17	3741.5
18	3523.0
19	3523.0
20	4106.5
21	4690.0
22	4252.0
23	3814.0
24	4302.0
25	4790.0
26	4790.0
27	5484.0
28	6178.0
29	7210.5
30	8243.0
31	9733.0
32	11223.0
33	11223.0
34	12859.5
35	14496.0
36	16948.0
37	19400.0
38	21144.5
39	22889.0
40	22889.0
41	24813.0
42	26737.0
43	28118.0
44	29499.0
45	31518.5
46	33538.0
47	33538.0
48	36148.0
49	38758.0
50	39241.0
51	39724.0
52	40906.0
53	42088.0
54	42088.0
55	40345.0
56	38602.0
57	37668.0
58	36734.0
59	36400.5
60	36067.0
61	36067.0
62	33894.5
63	31722.0
64	29578.5
65	27435.0
66	24246.5
67	21058.0
68	21058.0
69	18744.0
70	16430.0
71	13879.5
72	11329.0
73	8261.5
74	5194.0
75	5194.0
76	4110.5
77	3027.0
78	2546.5
79	2066.0
80	1841.5
81	1617.0
82	1617.0
83	1377.0
84	1137.0
85	955.5
86	774.0
87	686.5
88	599.0
89	599.0
90	454.0
91	309.0
92	183.5
93	58.0
94	38.0
95	18.0
96	18.0
97	12.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	553061.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.77617869289792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.08796003413948	53.85113997079368
2	4.922698747065104	5.885202399117366
3	1.484748998161346	2.6625786428458134
4	0.7529722108654876	1.8003920570992722
5	0.5026207602172721	1.5022374188753926
6	0.3517393817398815	1.2615381681727555
7	0.2525478486930367	1.0567441732387335
8	0.18730124376841967	0.895692209352247
9	0.15251128968965	0.8204887894655556
>10	1.0614694370970654	12.462752849545682
>50	0.14235497944203754	5.999606438015142
>100	0.09773625302411655	10.108630028228301
>500	0.0024282298887979233	0.8469520223241855
>1k	9.105862082992212E-4	0.846044832925809
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1792	0.32401489166656117	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1645	0.29743554508453857	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1226	0.22167536673169866	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	734	0.13271592102860263	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.1310886140950094	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	555	0.10035059423824859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	1.808118815103578E-4
14	0.0	0.0	0.0	0.0	1.808118815103578E-4
15	0.0	0.0	0.0	3.616237630207156E-4	1.808118815103578E-4
16	0.0	0.0	0.0	7.232475260414312E-4	1.808118815103578E-4
17	0.0	0.0	0.0	0.001808118815103578	1.808118815103578E-4
18	0.0	0.0	0.0	0.0021697425781242938	1.808118815103578E-4
19	0.0	0.0	0.0	0.0023505544596346514	1.808118815103578E-4
20	0.0	0.0	0.0	0.0027121782226553672	1.808118815103578E-4
21	0.0	0.0	0.0	0.0032546138671864407	1.808118815103578E-4
22	0.0	0.0	0.0	0.005243544563800376	1.808118815103578E-4
23	0.0	0.0	0.0	0.007232475260414312	1.808118815103578E-4
24	0.0	0.0	0.0	0.01229520794270433	1.808118815103578E-4
25	0.0	0.0	0.0	0.017719564388015065	1.808118815103578E-4
26	0.0	0.0	0.0	0.030376396093740113	1.808118815103578E-4
27	0.0	0.0	0.0	0.09058675263668926	1.808118815103578E-4
28	0.0	0.0	0.0	0.28405546585277214	1.808118815103578E-4
29	0.0	0.0	0.0	0.5370112880857627	1.808118815103578E-4
30	0.0	0.0	0.0	0.8402328133786328	1.808118815103578E-4
31	0.0	0.0	0.0	1.531657448274241	1.808118815103578E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	25	0.005493909	29.599998	25
TTACGGC	40	5.9346414E-5	27.750002	18
TAGCGCC	35	8.8635524E-4	26.428572	29
ACCGTTA	35	8.8635524E-4	26.428572	8
CCGTTAT	40	0.0019299269	23.125002	9
TCTATAC	40	0.0019299269	23.125002	3
GGTATCA	825	0.0	23.096968	1
AATACTA	50	2.699647E-4	22.199999	5
GCCCCTA	45	0.003823335	20.555557	27
AGATAAC	100	1.2862074E-8	20.349998	1
TATACAC	375	0.0	18.746666	37
TTCTATA	60	9.2285575E-4	18.5	2
CTAAGCC	70	1.2177462E-4	18.5	3
CCGATTT	50	0.0070303814	18.499998	20
TTAGCAA	50	0.0070303814	18.499998	4
TTGACCG	50	0.0070303814	18.499998	33
ATTGCCG	50	0.0070303814	18.499998	11
CAGTCGG	165	3.6379788E-12	17.939394	10
GCATTTG	190	0.0	17.526316	24
GTATCAA	1090	0.0	17.481651	2
>>END_MODULE