FastQCFastQC Report
Fri 10 Feb 2017
ERR1631534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109550
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12181.1118210862619808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7870.7183934276586034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7710.7037882245549978No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.25559105431309903No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2360.21542674577818346No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1510.1378366042902784No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1370.12505705157462346No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1330.12140575079872204No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1220.11136467366499317No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1180.10771337288909173No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.10497489730716568No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1100.10041077133728891No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTC250.005474931629.618
TTTGCGT358.8180625E-426.428573
GCAGTCG2450.025.6734689
CAGTCGG2450.025.67346810
GGCAGTC2500.025.168
GGGCAGT2500.025.167
AGTCGGT2650.023.7358511
AGGCGCG400.001920116523.12514
TTTTGCG400.001920116523.1252
TGGGCAG2800.022.4642856
GGGTATC603.6868325E-521.5833341
GAGGGGA450.003804085820.5555559
GGGGAAA555.102391E-420.18181811
TATAGGG555.102391E-420.1818183
TATACAC2150.019.79069737
GTCGGTG3200.019.6562512
TCGGTGA3250.019.35384613
TTCCTCG2200.019.34090820
ATTCCTC3100.019.09677519
CGGTGAT3300.019.06060614
Produced by FastQC (version 0.11.5)