##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631534.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	109550
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.776951163852125	34.0	31.0	34.0	31.0	34.0
2	32.94791419443177	34.0	33.0	34.0	31.0	34.0
3	33.01011410314925	34.0	33.0	34.0	31.0	34.0
4	36.38108626198083	37.0	37.0	37.0	35.0	37.0
5	36.39141944317663	37.0	37.0	37.0	35.0	37.0
6	36.47430397078959	37.0	37.0	37.0	35.0	37.0
7	36.46164308534915	37.0	37.0	37.0	35.0	37.0
8	36.46303970789594	37.0	37.0	37.0	35.0	37.0
9	38.3183477863989	39.0	39.0	39.0	37.0	39.0
10	38.27989958922866	39.0	39.0	39.0	37.0	39.0
11	38.33056138749429	39.0	39.0	39.0	37.0	39.0
12	38.1244089456869	39.0	39.0	39.0	37.0	39.0
13	38.246261980830674	39.0	39.0	39.0	37.0	39.0
14	39.69003194888179	41.0	39.0	41.0	37.0	41.0
15	39.67196713829302	41.0	39.0	41.0	37.0	41.0
16	39.62990415335463	41.0	39.0	41.0	37.0	41.0
17	39.67745321770881	41.0	39.0	41.0	38.0	41.0
18	39.73953445915107	41.0	40.0	41.0	38.0	41.0
19	39.78611592879963	41.0	40.0	41.0	38.0	41.0
20	39.78576905522593	41.0	40.0	41.0	38.0	41.0
21	39.77317206754906	41.0	40.0	41.0	38.0	41.0
22	39.728690095846645	41.0	40.0	41.0	37.0	41.0
23	39.6665905979005	41.0	40.0	41.0	37.0	41.0
24	39.64836147877681	41.0	40.0	41.0	37.0	41.0
25	39.63023277042446	41.0	40.0	41.0	37.0	41.0
26	39.54104974897307	41.0	39.0	41.0	37.0	41.0
27	39.46107713372889	41.0	39.0	41.0	36.0	41.0
28	39.37296211775445	41.0	39.0	41.0	36.0	41.0
29	39.25044272021908	41.0	39.0	41.0	35.0	41.0
30	39.14030123231401	40.0	39.0	41.0	35.0	41.0
31	39.01842081241442	40.0	39.0	41.0	35.0	41.0
32	38.90808763121862	40.0	38.0	41.0	35.0	41.0
33	38.81135554541305	40.0	38.0	41.0	35.0	41.0
34	38.74415335463259	40.0	38.0	41.0	35.0	41.0
35	38.623842994066635	40.0	38.0	41.0	35.0	41.0
36	38.54829758101324	40.0	38.0	41.0	35.0	41.0
37	38.45011410314925	40.0	38.0	41.0	35.0	41.0
38	38.344372432679144	40.0	38.0	41.0	35.0	41.0
39	38.22891830214514	40.0	38.0	41.0	35.0	41.0
40	38.11897763578275	40.0	38.0	41.0	35.0	41.0
41	37.96334094020995	40.0	38.0	41.0	34.0	41.0
42	37.81555454130534	40.0	38.0	41.0	34.0	41.0
43	36.953427658603374	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	1.0
16	1.0
17	1.0
18	2.0
19	6.0
20	9.0
21	14.0
22	22.0
23	38.0
24	66.0
25	120.0
26	172.0
27	272.0
28	405.0
29	579.0
30	719.0
31	971.0
32	1173.0
33	1575.0
34	2319.0
35	3296.0
36	5098.0
37	11347.0
38	22690.0
39	58650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.548607941579185	17.747147421268828	16.523961661341854	23.180282975810133
2	18.883614787768146	21.287996348699224	35.900502053856684	23.927886809675947
3	18.14696485623003	24.191693290734825	32.14240073026016	25.51894112277499
4	12.704701049748973	18.737562756732085	35.00867183934277	33.54906435417618
5	13.08443633044272	38.35508900045641	33.11729803742583	15.443176631675035
6	28.440894568690094	36.546782291191235	17.96074851665906	17.051574623459608
7	26.691921497033317	32.757644910999545	20.65814696485623	19.89228662711091
8	28.950251026928342	32.364217252396166	19.46143313555454	19.22409858512095
9	27.633044272021905	14.01551802829758	18.86444545869466	39.48699224098585
10	19.068005476951164	27.19397535371976	28.10862619808307	25.629392971246006
11	33.013235965312646	24.430853491556366	19.30442720219078	23.25148334094021
12	22.910999543587405	30.753080785029667	25.643998174349612	20.69192149703332
13	31.7480602464628	19.76814240073026	25.883158375171156	22.600638977635786
14	23.43678685531721	21.727065267001368	26.151528982199906	28.684618895481517
15	26.858968507530808	26.485623003194885	23.18667275216796	23.468735737106343
16	22.471930625285257	27.178457325422183	25.512551346417162	24.837060702875398
17	19.60657234139662	30.016430853491556	25.73893199452305	24.638064810588773
18	21.65586490187129	25.06070287539936	28.946599726152446	24.336832496576903
19	22.910086718393426	27.203103605659518	29.005020538566868	20.881789137380192
20	25.34002738475582	23.929712460063897	29.65951620264719	21.07074395253309
21	28.34322227293473	23.65221360109539	26.82336832496577	21.181195801004108
22	26.28206298493839	24.386125057051576	25.593792788680968	23.738019169329075
23	23.79552715654952	25.88041989958923	26.17252396166134	24.151528982199906
24	21.748060246462803	26.622546782291188	28.800547695116386	22.828845276129623
25	21.826563213144684	25.65495207667732	27.96896394340484	24.549520766773163
26	24.100410771337287	25.06709265175719	27.58192606115929	23.250570515746237
27	23.35006846188955	25.590141487905065	26.551346417161113	24.508443633044273
28	21.802829758101325	24.91921497033318	30.314924691921497	22.963030579644
29	22.757644910999545	25.393884071200368	30.06572341396623	21.782747603833865
30	22.342309447740757	25.476951163852124	30.1332724783204	22.04746691008672
31	22.577818347786398	25.823824737562756	28.432679141944316	23.165677772706527
32	21.267914194431768	24.91008671839343	31.17206754906435	22.64993153811045
33	21.499771793701505	24.704701049748973	31.424007302601552	22.37151985394797
34	22.679141944317664	23.71154723870379	29.594705613874943	24.014605203103606
35	20.627110908261066	24.401643085349157	30.17891373801917	24.792332268370608
36	19.19853947968964	23.908717480602466	30.59333637608398	26.299406663623913
37	19.54632587859425	23.009584664536742	32.056595162026476	25.38749429484254
38	19.236878137836605	21.519853947968965	33.637608397991784	25.605659516202646
39	18.62802373345504	21.847558192606115	35.48790506617983	24.036513007759012
40	18.424463715198538	22.256503879507076	34.456412596987676	24.86261980830671
41	16.396166134185304	23.30899132816066	35.86764034687357	24.427202190780463
42	16.152441807393885	23.599269739844818	34.41989958922866	25.82838886353263
43	14.986764034687358	24.575992697398448	34.62254678229119	25.814696485623003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	12.0
2	14.0
3	55.0
4	96.0
5	96.0
6	154.0
7	212.0
8	230.5
9	249.0
10	368.0
11	487.0
12	487.0
13	756.0
14	1025.0
15	1668.0
16	2311.0
17	1982.5
18	1654.0
19	1654.0
20	1751.5
21	1849.0
22	1403.0
23	957.0
24	962.5
25	968.0
26	968.0
27	1104.5
28	1241.0
29	1484.0
30	1727.0
31	1993.5
32	2260.0
33	2260.0
34	2777.0
35	3294.0
36	3793.5
37	4293.0
38	4820.0
39	5347.0
40	5347.0
41	6156.0
42	6965.0
43	8017.0
44	9069.0
45	9930.5
46	10792.0
47	10792.0
48	11330.5
49	11869.0
50	10792.5
51	9716.0
52	8622.0
53	7528.0
54	7528.0
55	6566.0
56	5604.0
57	5096.0
58	4588.0
59	4164.5
60	3741.0
61	3741.0
62	3435.0
63	3129.0
64	2834.0
65	2539.0
66	2203.5
67	1868.0
68	1868.0
69	1571.0
70	1274.0
71	1108.0
72	942.0
73	810.0
74	678.0
75	678.0
76	603.5
77	529.0
78	461.5
79	394.0
80	279.0
81	164.0
82	164.0
83	123.0
84	82.0
85	61.0
86	40.0
87	34.0
88	28.0
89	28.0
90	21.0
91	14.0
92	7.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	109550.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.86353263350068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.81210664992014	58.04564125969877
2	6.543434040055154	8.75034230944774
3	2.4164152411637017	4.847101780009129
4	1.2382421603025298	3.311729803742583
5	0.7822632390885882	2.6152441807393885
6	0.47782222282898057	1.9169329073482428
7	0.3481276194896859	1.6293929712460065
8	0.25529358762576965	1.365586490187129
9	0.18157244467501263	1.0926517571884984
>10	0.8696364455487446	9.62482884527613
>50	0.05324304768665784	2.6033774532177087
>100	0.01774768256221928	1.6631675034230946
>500	0.002730412701879889	1.422181652213601
>1k	0.0013652063509399445	1.1118210862619808
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1218	1.1118210862619808	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	787	0.7183934276586034	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.7037882245549978	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	280	0.25559105431309903	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	236	0.21542674577818346	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	151	0.1378366042902784	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	137	0.12505705157462346	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	133	0.12140575079872204	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	122	0.11136467366499317	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	118	0.10771337288909173	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.10497489730716568	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	110	0.10041077133728891	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.008215426745778184	0.0
4	0.0	0.0	0.0	0.008215426745778184	0.0
5	0.0	0.0	0.0	0.008215426745778184	0.0
6	0.0	0.0	0.0	0.008215426745778184	0.0
7	0.0	0.0	0.0	0.008215426745778184	0.0
8	0.0	0.0	0.0	0.01460520310360566	0.0
9	0.0	0.0	0.0	0.019169329073482427	0.0
10	0.0	0.0	0.0	0.028297581013235967	0.0
11	0.0	0.0	0.0	0.037425832952989506	0.0
12	0.0	0.0	0.0	0.04198995892286627	0.0
13	0.0	0.0	0.0	0.045641259698767686	0.0
14	0.0	0.0	0.0	0.045641259698767686	0.0
15	0.0	0.0	0.0	0.05020538566864446	0.0
16	0.0	0.0	0.0	0.0611592879963487	0.0
17	0.0	0.0	0.0	0.06937471474212688	0.0
18	0.0	0.0	0.0	0.07120036513007759	0.0
19	0.0	0.0	0.0	0.08032861706983113	0.0
20	0.0	0.0	0.0	0.08671839342765861	0.0
21	0.0	0.0	0.0	0.09219534459151073	0.0
22	0.0	0.0	0.0	0.12323140118667275	0.0
23	0.0	0.0	0.0	0.16704701049748974	0.0
24	0.0	0.0	0.0	0.2190780465540849	0.0
25	0.0	0.0	0.0	0.23550890004564126	0.0
26	0.0	0.0	0.0	0.2665449566408033	0.0
27	0.0	0.0	0.0	0.38703788224554997	0.0
28	0.0	0.0	0.0	0.848927430397079	0.0
29	0.0	0.0	0.0	1.7334550433591966	0.0
30	0.0	0.0	0.0	3.1967138293016886	0.0
31	0.0	0.0	0.0	5.748060246462803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCCTC	25	0.0054749316	29.6	18
TTTGCGT	35	8.8180625E-4	26.42857	3
GCAGTCG	245	0.0	25.673468	9
CAGTCGG	245	0.0	25.673468	10
GGCAGTC	250	0.0	25.16	8
GGGCAGT	250	0.0	25.16	7
AGTCGGT	265	0.0	23.73585	11
AGGCGCG	40	0.0019201165	23.125	14
TTTTGCG	40	0.0019201165	23.125	2
TGGGCAG	280	0.0	22.464285	6
GGGTATC	60	3.6868325E-5	21.583334	1
GAGGGGA	45	0.0038040858	20.555555	9
GGGGAAA	55	5.102391E-4	20.181818	11
TATAGGG	55	5.102391E-4	20.181818	3
TATACAC	215	0.0	19.790697	37
GTCGGTG	320	0.0	19.65625	12
TCGGTGA	325	0.0	19.353846	13
TTCCTCG	220	0.0	19.340908	20
ATTCCTC	310	0.0	19.096775	19
CGGTGAT	330	0.0	19.060606	14
>>END_MODULE