FastQCFastQC Report
Fri 10 Feb 2017
ERR1631533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522337
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.16713347896090072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8660.16579334797266898No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA7290.13956507006013358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6690.1280782330181473No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5580.10682758449047262No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT5540.10606179535434021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG200.001841016737.04
GCCGTAT352.3858316E-531.7142878
GCCCTAA250.00549363229.61
TATAGAC250.00549363229.63
ACTGTAG405.9340196E-527.7500028
GGTATCA4400.023.9659081
ACTGATC1500.022.28
CCGTATG502.6993678E-422.29
GTTCTAG1350.021.9259261
TCTAGAC1251.8189894E-1120.723
CGTATGA555.1379314E-420.18181810
CTAGACA1202.382876E-1020.0416684
CTCTAGT1153.0395313E-919.30434827
TATGACA701.2175817E-418.512
TATACAA500.00702986918.52
ATACACA1306.9303496E-1018.537
AGTGACG1054.784888E-717.61904719
GACGAAA1054.784888E-717.61904722
TAGTGAC1054.784888E-717.61904718
CTAGAAC2000.017.5753
Produced by FastQC (version 0.11.5)