##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631533.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522337
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.374139683767375	33.0	31.0	34.0	31.0	34.0
2	32.572856221175215	34.0	31.0	34.0	31.0	34.0
3	32.699205685218544	34.0	31.0	34.0	31.0	34.0
4	36.20512810695011	37.0	37.0	37.0	35.0	37.0
5	36.02315363453096	37.0	35.0	37.0	35.0	37.0
6	36.03793145038548	37.0	35.0	37.0	35.0	37.0
7	36.03787018725459	37.0	35.0	37.0	35.0	37.0
8	36.016083486331624	37.0	35.0	37.0	35.0	37.0
9	37.77759760461158	39.0	37.0	39.0	35.0	39.0
10	37.659593327679254	39.0	37.0	39.0	35.0	39.0
11	37.73778039847838	39.0	37.0	39.0	35.0	39.0
12	37.51404935893877	39.0	37.0	39.0	35.0	39.0
13	37.65869161097146	39.0	37.0	39.0	35.0	39.0
14	39.0049699714935	40.0	38.0	41.0	36.0	41.0
15	38.972169308320105	40.0	38.0	41.0	36.0	41.0
16	38.94056327619908	40.0	38.0	41.0	36.0	41.0
17	38.93021172155141	40.0	38.0	41.0	36.0	41.0
18	38.907741553824444	40.0	38.0	41.0	36.0	41.0
19	38.902354227251756	40.0	38.0	41.0	36.0	41.0
20	38.88046414479541	40.0	38.0	41.0	36.0	41.0
21	38.84911273756215	40.0	38.0	41.0	35.0	41.0
22	38.79208824954005	40.0	38.0	41.0	35.0	41.0
23	38.73367768318155	40.0	38.0	41.0	35.0	41.0
24	38.69249545791319	40.0	38.0	41.0	35.0	41.0
25	38.682798652976906	40.0	38.0	41.0	35.0	41.0
26	38.585039926331085	40.0	38.0	41.0	35.0	41.0
27	38.50988920945673	40.0	38.0	41.0	35.0	41.0
28	38.41778009216272	40.0	38.0	41.0	34.0	41.0
29	38.326538231065385	40.0	38.0	41.0	34.0	41.0
30	38.271010860804424	40.0	38.0	41.0	34.0	41.0
31	38.21689445702678	40.0	38.0	41.0	34.0	41.0
32	38.138372353480605	40.0	38.0	41.0	34.0	41.0
33	38.03314718275749	40.0	38.0	41.0	33.0	41.0
34	37.993107897774806	40.0	37.0	41.0	33.0	41.0
35	37.892107968610304	40.0	37.0	41.0	33.0	41.0
36	37.82962340404758	40.0	37.0	41.0	33.0	41.0
37	37.767933345713594	40.0	37.0	41.0	33.0	41.0
38	37.67842408253675	40.0	37.0	41.0	33.0	41.0
39	37.594520395836405	40.0	37.0	41.0	33.0	41.0
40	37.49389187440293	40.0	36.0	41.0	33.0	41.0
41	37.39229845865792	39.0	36.0	41.0	33.0	41.0
42	37.27092088058093	39.0	36.0	41.0	32.0	41.0
43	36.28444471672503	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	2.0
15	3.0
16	8.0
17	11.0
18	19.0
19	34.0
20	58.0
21	118.0
22	213.0
23	376.0
24	586.0
25	809.0
26	1217.0
27	1850.0
28	2461.0
29	3483.0
30	4744.0
31	6430.0
32	8514.0
33	11790.0
34	17065.0
35	26049.0
36	44295.0
37	83137.0
38	163487.0
39	145575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.816348449372725	18.035291392338664	11.89787436080538	26.250485797483236
2	19.9895852677486	19.960868175143634	32.64405929505281	27.405487262054955
3	20.75709742943732	21.077197288340667	28.748298512263155	29.41740676995886
4	15.210678163714231	15.105765052064088	33.11406237735408	36.5694944068676
5	16.565359145532483	34.604479483551806	32.05650758035521	16.7736537905605
6	35.891962468674436	33.44488328416329	15.301424176345924	15.361730070816352
7	31.06442775449566	29.295263402745736	19.796606405443228	19.843702437315372
8	28.866230039227545	30.942092939998506	19.461573658385294	20.73010336238865
9	27.313975460287132	13.721218293936674	19.068340936981297	39.8964653087949
10	18.784998956612302	24.910354809251498	30.853835742059243	25.450810492076954
11	38.29003114847311	20.45499361523308	19.937511606491594	21.317463629802216
12	23.61061919795075	24.42809910077211	26.841100668725364	25.12018103255178
13	31.351215785977253	17.944736826991004	23.983367059963204	26.720680327068543
14	24.260965621811206	19.48454733246927	23.016941170164092	33.23754587555543
15	27.1160189685969	26.02151484577964	20.726465863992022	26.136000321631435
16	26.89834340665126	24.394021484214214	22.547895324282983	26.159739784851542
17	25.698543277615794	24.697465429406687	23.3431673421565	26.26082395082102
18	26.189031219308607	22.762124835116026	24.855983780586097	26.192860164989266
19	26.071099692344212	23.760139526780602	24.05458544962352	26.114175331251666
20	26.322087081711615	23.0649944384564	24.310933362943846	26.301985116888137
21	27.745497638497753	23.416874546509245	23.806086874948548	25.031540940044454
22	27.540840491866366	23.050061550301816	23.44061400972935	25.968483948102474
23	26.313854848498192	23.070929304261426	24.007872312319442	26.60734353492094
24	25.85055242113808	23.654652073278363	23.77411517851502	26.720680327068543
25	26.48883766610445	23.101752316990755	23.828103312612356	26.58130670429244
26	26.39751731162066	23.863138165590414	23.694856002925317	26.044488519863613
27	26.74173952831218	22.98611815743476	23.91521182684742	26.356930487405638
28	25.790055079383617	23.004879991270002	24.8808719275104	26.324193001835976
29	25.300332926826936	23.444442955410015	24.61935493752118	26.635869180241873
30	24.971617940142092	24.250436021189387	24.950941633466517	25.827004405202004
31	26.508748183643892	23.68049745662283	23.510492268401435	26.300262091331838
32	25.79828731259704	23.45573834516797	23.86792434769124	26.87804999454375
33	25.20441783752635	23.036468793135466	24.767726582646834	26.99138678669135
34	26.346017992215753	22.772080093885748	24.547370758724732	26.334531155173767
35	25.810539938775158	23.01770695930022	25.381315127972936	25.79043797395168
36	25.12937050218537	23.43755085318482	24.89503902652885	26.538039618100957
37	25.756934699245885	22.71617748694808	25.289994773489145	26.236893040316883
38	24.903462707026307	22.272594129843377	26.029938526277096	26.79400463685322
39	25.379592102416638	22.1408783984286	26.11340954211553	26.36611995703923
40	24.829372608105498	22.791799164141157	26.4752449089381	25.903583318815247
41	24.28221627033888	22.550575586259445	27.12099659798176	26.04621154541991
42	24.834924579342456	22.67731368828936	26.94505654395534	25.542705188412846
43	23.409216655147922	21.880892986711643	27.268602453971287	27.441287904169148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	90.0
1	81.5
2	73.0
3	134.5
4	196.0
5	196.0
6	228.0
7	260.0
8	246.5
9	233.0
10	301.5
11	370.0
12	370.0
13	653.0
14	936.0
15	1335.0
16	1734.0
17	1722.5
18	1711.0
19	1711.0
20	2092.5
21	2474.0
22	2298.5
23	2123.0
24	2483.5
25	2844.0
26	2844.0
27	3401.5
28	3959.0
29	4920.5
30	5882.0
31	6912.0
32	7942.0
33	7942.0
34	9896.5
35	11851.0
36	14501.0
37	17151.0
38	19131.5
39	21112.0
40	21112.0
41	23508.5
42	25905.0
43	28026.0
44	30147.0
45	32495.0
46	34843.0
47	34843.0
48	36991.0
49	39139.0
50	39941.5
51	40744.0
52	41516.0
53	42288.0
54	42288.0
55	41285.0
56	40282.0
57	38853.5
58	37425.0
59	36373.0
60	35321.0
61	35321.0
62	33254.0
63	31187.0
64	28520.0
65	25853.0
66	23078.5
67	20304.0
68	20304.0
69	16862.0
70	13420.0
71	11557.5
72	9695.0
73	7530.5
74	5366.0
75	5366.0
76	4274.5
77	3183.0
78	2606.0
79	2029.0
80	1812.5
81	1596.0
82	1596.0
83	1317.5
84	1039.0
85	891.0
86	743.0
87	642.0
88	541.0
89	541.0
90	408.0
91	275.0
92	158.5
93	42.0
94	31.0
95	20.0
96	20.0
97	14.5
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	522337.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.397034932099714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.77404968228278	53.01292344792689
2	6.221221809043566	7.514867018422897
3	2.0826628532880562	3.773599833054756
4	0.9928360296329642	2.398574094543573
5	0.5972716926979346	1.803671964391574
6	0.3851554421236903	1.3957348015339701
7	0.29230701643593987	1.2358133957805515
8	0.20366887283881363	0.9840796821941762
9	0.15473813409250753	0.8411152041101797
>10	1.0978064183148035	12.750600003383122
>50	0.12138420366056592	5.125922740533735
>100	0.0746735277407404	8.24880316575322
>500	0.0022243178475965216	0.9142946483713346
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	873	0.16713347896090072	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	866	0.16579334797266898	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	729	0.13956507006013358	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	669	0.1280782330181473	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	558	0.10682758449047262	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	554	0.10606179535434021	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.9144728403310506E-4
10	0.0	0.0	0.0	0.0	1.9144728403310506E-4
11	0.0	1.9144728403310506E-4	0.0	1.9144728403310506E-4	1.9144728403310506E-4
12	0.0	1.9144728403310506E-4	0.0	1.9144728403310506E-4	1.9144728403310506E-4
13	0.0	1.9144728403310506E-4	0.0	3.828945680662101E-4	1.9144728403310506E-4
14	0.0	1.9144728403310506E-4	0.0	3.828945680662101E-4	1.9144728403310506E-4
15	0.0	1.9144728403310506E-4	0.0	5.743418520993152E-4	1.9144728403310506E-4
16	0.0	1.9144728403310506E-4	0.0	7.657891361324202E-4	1.9144728403310506E-4
17	0.0	1.9144728403310506E-4	0.0	0.0011486837041986303	1.9144728403310506E-4
18	0.0	1.9144728403310506E-4	0.0	0.0011486837041986303	1.9144728403310506E-4
19	0.0	1.9144728403310506E-4	0.0	0.0015315782722648405	1.9144728403310506E-4
20	0.0	1.9144728403310506E-4	0.0	0.0017230255562979456	1.9144728403310506E-4
21	0.0	1.9144728403310506E-4	0.0	0.0021059201243641555	1.9144728403310506E-4
22	0.0	1.9144728403310506E-4	0.0	0.0034460511125958913	1.9144728403310506E-4
23	0.0	1.9144728403310506E-4	0.0	0.005360523952926942	1.9144728403310506E-4
24	0.0	1.9144728403310506E-4	0.0	0.007657891361324202	1.9144728403310506E-4
25	0.0	1.9144728403310506E-4	0.0	0.008998022349555938	1.9144728403310506E-4
26	0.0	1.9144728403310506E-4	0.0	0.012635520746184934	1.9144728403310506E-4
27	0.0	1.9144728403310506E-4	0.0	0.038863798658720326	1.9144728403310506E-4
28	0.0	1.9144728403310506E-4	0.0	0.12692954931394865	1.9144728403310506E-4
29	0.0	3.828945680662101E-4	0.0	0.24352094529010965	1.9144728403310506E-4
30	0.0	3.828945680662101E-4	0.0	0.4029965328896862	1.9144728403310506E-4
31	0.0	3.828945680662101E-4	0.0	0.7661720307004865	1.9144728403310506E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	20	0.0018410167	37.0	4
GCCGTAT	35	2.3858316E-5	31.714287	8
GCCCTAA	25	0.005493632	29.6	1
TATAGAC	25	0.005493632	29.6	3
ACTGTAG	40	5.9340196E-5	27.750002	8
GGTATCA	440	0.0	23.965908	1
ACTGATC	150	0.0	22.2	8
CCGTATG	50	2.6993678E-4	22.2	9
GTTCTAG	135	0.0	21.925926	1
TCTAGAC	125	1.8189894E-11	20.72	3
CGTATGA	55	5.1379314E-4	20.181818	10
CTAGACA	120	2.382876E-10	20.041668	4
CTCTAGT	115	3.0395313E-9	19.304348	27
TATGACA	70	1.2175817E-4	18.5	12
TATACAA	50	0.007029869	18.5	2
ATACACA	130	6.9303496E-10	18.5	37
AGTGACG	105	4.784888E-7	17.619047	19
GACGAAA	105	4.784888E-7	17.619047	22
TAGTGAC	105	4.784888E-7	17.619047	18
CTAGAAC	200	0.0	17.575	3
>>END_MODULE