FastQCFastQC Report
Fri 10 Feb 2017
ERR1631532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61728
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3460.560523587350959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.4681829963711768No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2200.35640228097459825No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA990.16038102643856922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.15876101607050283No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA910.14742094349403836No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC750.12150077760497667No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC650.10530067392431311No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC630.1020606531881804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATC250.00545662229.625
GCAGTCG701.1956449E-418.59
TATGCCC609.102493E-418.536
GATATGC701.1956449E-418.534
GGTATCA2300.018.51
CAGTCGG701.1956449E-418.510
TCGGTGA701.1956449E-418.513
AGTCGGT701.1956449E-418.511
GGCAGTC752.0276142E-417.2666668
GTGATTC752.0276142E-417.26666616
CTTATTG752.0276142E-417.26666628
GGGCAGT752.0276142E-417.2666667
TTATTGA752.0276142E-417.26666629
GTCGGTG803.3181466E-416.187512
ATATGCC700.002555407615.85714235
TGATTCC956.888254E-515.57894817
TGATATG855.2627205E-415.23529433
CTCTATG1101.4080542E-515.1363641
ATTCCTC750.00404617514.819
TTGATAT908.1186835E-414.38888932
Produced by FastQC (version 0.11.5)