FastQCFastQC Report
Fri 10 Feb 2017
ERR1631531.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631531.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760140
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101270.5753519606394946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80460.4571227288738396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75130.42684104673491885No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33990.19310963900598815No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA24330.1382276409831036No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA22850.12981921892576728No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA20980.11919506402899768No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC20530.11663844921426705No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC20410.1159566852636722No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC19680.11180928789755361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.022.9810541
GTATCGA450.003826604220.5555551
GCAGTCG9450.019.9682529
AGTCGGT9450.019.77248611
CAGTCGG9850.018.78172710
TAACGCT500.00703633418.49999832
CGGTGAT10450.018.41148414
TATACAC13100.018.35877837
GCCTTAT8500.018.28235426
GGCAGTC10950.017.5707788
CGCCTTA9000.017.47222325
TCGGTGA10750.017.38139513
GTCGGTG10850.017.22119912
CTTATAC39850.017.1304937
ACCGTGA1107.813396E-716.8181828
GGGCAGT11350.016.7885467
TCCTCGC9300.016.70967921
CTTATTG11350.016.62555128
TTCCTCG9750.016.50769220
AGCGTCA1801.0913936E-1116.4444453
Produced by FastQC (version 0.11.5)