FastQCFastQC Report
Fri 10 Feb 2017
ERR1631530.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631530.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62063
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5000.8056329858369722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.7734076664034932No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4470.7202358893382531No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3350.5397741005107713No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2100.3383658540515283No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2020.32547572627813676No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1900.3061405346180494No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1820.29325040684465786No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1740.2803602790712663No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1680.2706926832412226No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1520.24491242769443955No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1460.23524483186439585No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1380.2223547040910043No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1340.2159096402043085No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1330.2142983742326346No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1310.2110758422892867No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.19818571451589514No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT1070.17240545896911205No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1020.16434912911074231No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1020.16434912911074231No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC960.15468153328069864No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG930.1498477353656768No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA850.13695760759228526No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.13212380967726345No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC800.12890127773391555No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT740.11923368190387187No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC720.11601114996052399No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT680.10956608607382821No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.10312102218713243No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA630.10150975621545848ABI Solid3 Adapter B (100% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG200.001828550537.017
ATGCCCC651.8189894E-1234.15384737
TATTGAC303.5607204E-430.83333430
ATTGACT303.5607204E-430.83333431
TGACTGT358.7747927E-426.42857233
CTTGATC358.7747927E-426.4285723
AGTCGGT3150.024.07936711
CAGTCGG3200.023.70312710
GCAGTCG3200.023.7031279
TTGACTG400.001910783723.12500232
GGTGATT3300.022.98484815
GGGCAGT3300.022.9848487
GGCAGTC3300.022.9848488
TCGCCTT2200.022.70454624
CGCCTTA2250.022.19999925
TGATTCC3250.022.19999917
TGGGCAG3450.021.9855066
CTCGCCT2300.021.71739223
CGGTGAT3500.021.67142914
GTGATTC3500.021.67142916
Produced by FastQC (version 0.11.5)