##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631530.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62063
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77998163156792	34.0	31.0	34.0	31.0	34.0
2	32.92415771071331	34.0	33.0	34.0	31.0	34.0
3	32.99545622995988	34.0	33.0	34.0	31.0	34.0
4	36.39495351497672	37.0	37.0	37.0	35.0	37.0
5	36.374893253629374	37.0	37.0	37.0	35.0	37.0
6	36.435444628844884	37.0	37.0	37.0	35.0	37.0
7	36.417350111982984	37.0	37.0	37.0	35.0	37.0
8	36.41983146157936	37.0	37.0	37.0	35.0	37.0
9	38.24866667740844	39.0	39.0	39.0	37.0	39.0
10	38.20374458211817	39.0	39.0	39.0	37.0	39.0
11	38.25836649855792	39.0	39.0	39.0	37.0	39.0
12	38.044873757311116	39.0	39.0	39.0	37.0	39.0
13	38.1550682371139	39.0	39.0	39.0	37.0	39.0
14	39.62309266390603	41.0	39.0	41.0	37.0	41.0
15	39.62410776146819	41.0	39.0	41.0	37.0	41.0
16	39.55224529913153	41.0	39.0	41.0	37.0	41.0
17	39.463915698564364	41.0	39.0	41.0	37.0	41.0
18	39.556805181831365	41.0	39.0	41.0	37.0	41.0
19	39.588144304980425	41.0	39.0	41.0	37.0	41.0
20	39.56996922481994	41.0	40.0	41.0	37.0	41.0
21	39.5524064257287	41.0	40.0	41.0	37.0	41.0
22	39.49647938385189	41.0	39.0	41.0	37.0	41.0
23	39.418590786781174	41.0	39.0	41.0	37.0	41.0
24	39.375134943525126	41.0	39.0	41.0	36.0	41.0
25	39.33193690282455	41.0	39.0	41.0	36.0	41.0
26	39.200006445063885	41.0	39.0	41.0	36.0	41.0
27	39.08612216618597	41.0	39.0	41.0	35.0	41.0
28	38.991734205565315	40.0	39.0	41.0	35.0	41.0
29	38.85798301725666	40.0	38.0	41.0	35.0	41.0
30	38.69658250487408	40.0	38.0	41.0	35.0	41.0
31	38.52677118411936	40.0	38.0	41.0	35.0	41.0
32	38.37283083318563	40.0	37.0	41.0	35.0	41.0
33	38.21102750431014	40.0	37.0	41.0	34.0	41.0
34	38.083801943186764	40.0	37.0	41.0	34.0	41.0
35	37.918518279812446	40.0	36.0	41.0	34.0	41.0
36	37.761452072893675	40.0	36.0	41.0	33.0	41.0
37	37.57750994956738	40.0	35.0	41.0	33.0	41.0
38	37.389926365145094	39.0	35.0	41.0	33.0	41.0
39	37.172050980455346	39.0	35.0	41.0	33.0	41.0
40	36.98314615793629	39.0	35.0	41.0	33.0	41.0
41	36.78270467106005	39.0	35.0	41.0	32.0	41.0
42	36.5859046452798	39.0	35.0	41.0	32.0	41.0
43	35.487971899521455	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	1.0
19	4.0
20	10.0
21	17.0
22	20.0
23	39.0
24	61.0
25	98.0
26	155.0
27	233.0
28	288.0
29	411.0
30	517.0
31	661.0
32	833.0
33	1046.0
34	1575.0
35	2388.0
36	4237.0
37	8923.0
38	15244.0
39	25299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2040668353125	14.3837713291333	13.21560349966969	27.196558335884504
2	22.148462046630037	17.169650194157548	31.833781802362115	28.848105956850294
3	21.26709956012439	19.116059487939673	27.29162302821327	32.32521792372267
4	15.418204082947973	15.89513881056346	30.0597779675491	38.626879138939465
5	17.274382482316355	35.225496672735765	28.529075294458856	18.97104555048902
6	34.28451734527819	28.2728840049627	18.4280489180349	19.014549731724216
7	34.922578670061064	28.10531234390861	15.387590029486168	21.58451895654416
8	35.644425825370995	26.68739828883554	15.524547637078454	22.143628248715018
9	31.053929072071927	11.92014565844384	15.508434977361713	41.517490292122524
10	22.423988527786282	22.25480560076052	23.1587258108696	32.1624800605836
11	39.57591479625542	21.811707458550185	14.91065530187068	23.70172244332372
12	26.38125775421749	24.57663986594267	22.425599793757954	26.616502586081886
13	37.349145223402026	15.32313939061921	21.800428596748464	25.527286789230295
14	25.875320239111872	17.562799091245992	20.716046597811903	35.84583407183023
15	35.344730354639644	20.469522904145787	16.486473422167798	27.699273319046775
16	29.832589465543073	21.143032080305495	20.914232312327798	28.110146141823634
17	24.942397241512655	25.043906997728115	22.154907110516735	27.858788650242495
18	28.646697710391056	19.043552519214344	23.276348226801797	29.033401543592802
19	28.43562186810177	24.636256706894606	22.709182604772572	24.218938820231056
20	30.43198040700578	18.568229057570534	24.732932665195044	26.266857870228637
21	32.57657541530381	17.912443807099237	22.44010118750302	27.070879590093938
22	31.787055089183568	17.720703156470037	20.084430336915716	30.407811417430676
23	28.508128836827098	19.92008120780497	21.391167039943284	30.18062291542465
24	28.214878429982438	18.871147060245235	24.00141791405507	28.912556595717255
25	27.523645328134315	19.15472987125985	21.84715530992701	31.474469490678825
26	30.409422683402347	18.51827981244864	21.81815252243688	29.254144981712134
27	28.791711647841712	18.589175515202292	20.774052172792164	31.84506066416383
28	27.069268324122262	19.108003158081306	23.05721605465414	30.76551246314229
29	26.56655334095999	20.334176562525176	24.910171922079176	28.189098174435657
30	26.34097610492564	20.712824065868553	26.22496495496512	26.72123487424069
31	27.082158451895655	22.32086750559915	22.461047645134784	28.135926397370415
32	26.268469136200313	21.199426389314084	25.75447529123632	26.777629183249278
33	27.966743470344653	19.241738233730242	24.93111837971094	27.860399916214167
34	28.825548233246863	20.40184973333548	21.64413579749609	29.128466235921564
35	26.91619805681324	22.00989317306608	22.91220211720349	28.161706652917196
36	25.163140679631983	21.379888178141567	24.757101654770153	28.699869487456294
37	25.852762515508438	21.87615809741714	24.18349096885423	28.087588418220193
38	25.846317451621736	18.979101880347386	27.151442888677636	28.023137779353235
39	27.130496431045874	18.706797931134492	26.231410018851815	27.931295618967823
40	25.49506146979682	18.37487714096966	25.076132317161594	31.053929072071927
41	22.343425229202584	19.676780046082207	27.86523371412919	30.114561010586016
42	23.184506066416382	18.76158097417141	27.712163446820163	30.341749512592042
43	21.74081175579653	21.23809677263426	28.64347517844771	28.377616293121505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	15.0
4	30.0
5	30.0
6	35.0
7	40.0
8	43.5
9	47.0
10	66.5
11	86.0
12	86.0
13	194.0
14	302.0
15	542.0
16	782.0
17	690.0
18	598.0
19	598.0
20	747.5
21	897.0
22	578.5
23	260.0
24	221.5
25	183.0
26	183.0
27	190.0
28	197.0
29	231.5
30	266.0
31	291.5
32	317.0
33	317.0
34	340.0
35	363.0
36	374.5
37	386.0
38	530.5
39	675.0
40	675.0
41	820.0
42	965.0
43	1336.5
44	1708.0
45	2107.5
46	2507.0
47	2507.0
48	3972.0
49	5437.0
50	5692.0
51	5947.0
52	5034.5
53	4122.0
54	4122.0
55	3838.0
56	3554.0
57	3760.0
58	3966.0
59	4058.0
60	4150.0
61	4150.0
62	4322.5
63	4495.0
64	4434.0
65	4373.0
66	4295.0
67	4217.0
68	4217.0
69	3922.5
70	3628.0
71	3315.0
72	3002.0
73	2509.0
74	2016.0
75	2016.0
76	1613.0
77	1210.0
78	975.5
79	741.0
80	545.5
81	350.0
82	350.0
83	255.0
84	160.0
85	111.0
86	62.0
87	40.0
88	18.0
89	18.0
90	12.0
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	62063.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.48255804585663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.40583929462652	72.37323364967855
2	4.373232407253369	6.776984676860609
3	0.7798203293961071	1.8126742181331872
4	0.3597571119614041	1.1149960523983693
5	0.18091831641989684	0.7009006976781658
6	0.12893029446015639	0.5993909414627072
7	0.10605556479787058	0.5752219518875981
8	0.0769422725004159	0.4769347276154875
9	0.06862418898685743	0.47854599358716143
>10	0.4408584262185993	6.43861882280908
>50	0.03743137581101314	1.9560768896121683
>100	0.04159041756779238	6.696421378276912
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	500	0.8056329858369722	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	480	0.7734076664034932	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	447	0.7202358893382531	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	335	0.5397741005107713	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	210	0.3383658540515283	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	202	0.32547572627813676	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	190	0.3061405346180494	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	182	0.29325040684465786	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	174	0.2803602790712663	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	168	0.2706926832412226	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	152	0.24491242769443955	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	146	0.23524483186439585	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	138	0.2223547040910043	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	134	0.2159096402043085	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	133	0.2142983742326346	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	131	0.2110758422892867	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.19818571451589514	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	107	0.17240545896911205	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	102	0.16434912911074231	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	102	0.16434912911074231	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	96	0.15468153328069864	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	93	0.1498477353656768	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	85	0.13695760759228526	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.13212380967726345	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	80	0.12890127773391555	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	74	0.11923368190387187	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	72	0.11601114996052399	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	68	0.10956608607382821	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64	0.10312102218713243	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	63	0.10150975621545848	ABI Solid3 Adapter B (100% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0016112659716739442	0.0	0.0
12	0.0	0.0	0.0016112659716739442	0.0	0.0
13	0.0	0.0	0.0016112659716739442	0.0	0.0016112659716739442
14	0.0	0.0	0.0016112659716739442	0.0	0.0016112659716739442
15	0.0	0.0	0.0016112659716739442	0.0	0.0016112659716739442
16	0.0	0.0	0.0016112659716739442	0.0032225319433478884	0.0016112659716739442
17	0.0	0.0	0.0016112659716739442	0.0032225319433478884	0.0016112659716739442
18	0.0	0.0	0.0016112659716739442	0.0032225319433478884	0.0016112659716739442
19	0.0	0.0	0.0016112659716739442	0.0032225319433478884	0.0016112659716739442
20	0.0	0.0	0.0016112659716739442	0.0032225319433478884	0.0016112659716739442
21	0.0	0.0	0.0016112659716739442	0.006445063886695777	0.0016112659716739442
22	0.0	0.0	0.0016112659716739442	0.008056329858369722	0.0016112659716739442
23	0.0	0.0	0.0016112659716739442	0.016112659716739443	0.0016112659716739442
24	0.0	0.0	0.0016112659716739442	0.03383658540515283	0.0016112659716739442
25	0.0	0.0	0.0016112659716739442	0.03867038332017466	0.0016112659716739442
26	0.0	0.0	0.0016112659716739442	0.04833797915021833	0.0016112659716739442
27	0.0	0.0	0.0016112659716739442	0.07572950066867538	0.0016112659716739442
28	0.0	0.0	0.0016112659716739442	0.17723925688413386	0.0016112659716739442
29	0.0	0.0	0.0016112659716739442	0.41409535472020365	0.0016112659716739442
30	0.0	0.0	0.0016112659716739442	0.6461176546412516	0.0016112659716739442
31	0.0	0.0	0.0016112659716739442	1.2068382127837842	0.0016112659716739442
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAGCGG	20	0.0018285505	37.0	17
ATGCCCC	65	1.8189894E-12	34.153847	37
TATTGAC	30	3.5607204E-4	30.833334	30
ATTGACT	30	3.5607204E-4	30.833334	31
TGACTGT	35	8.7747927E-4	26.428572	33
CTTGATC	35	8.7747927E-4	26.428572	3
AGTCGGT	315	0.0	24.079367	11
CAGTCGG	320	0.0	23.703127	10
GCAGTCG	320	0.0	23.703127	9
TTGACTG	40	0.0019107837	23.125002	32
GGTGATT	330	0.0	22.984848	15
GGGCAGT	330	0.0	22.984848	7
GGCAGTC	330	0.0	22.984848	8
TCGCCTT	220	0.0	22.704546	24
CGCCTTA	225	0.0	22.199999	25
TGATTCC	325	0.0	22.199999	17
TGGGCAG	345	0.0	21.985506	6
CTCGCCT	230	0.0	21.717392	23
CGGTGAT	350	0.0	21.671429	14
GTGATTC	350	0.0	21.671429	16
>>END_MODULE