FastQCFastQC Report
Fri 10 Feb 2017
ERR1631529.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631529.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGC11.0No Hit
GGGCAGTGGCATGATCACTGTAGCTTCAACCTCCCGGGCTCAA11.0No Hit
GTATTTGTGGGGTTCAGATGGATATTAAAGTAGATGGACTTAG11.0No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC11.0No Hit
CACCAGTGATGTAATAGATGGACTTCTGTGTCTCCTTCATGCG11.0No Hit
TTGAGAAGCCTTCGCTTCGAAGCGAAAAGTCCTAATAGTAGAA11.0No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.0No Hit
GCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACACAGC11.0RNA PCR Primer, Index 40 (96% over 29bp)
CCCGCATGCTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTA11.0No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG11.0No Hit
GGCTGTAGGGTGGCTCAGTGGAATCCATTTTGTTAACACCGAC11.0No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCA11.0No Hit
CAACCAACTTGGTTCTGCTTCAATAAATCTTTGGAAAAACTTA11.0No Hit
CGTGTGAACCATGTGACTTTGTCACAGCCCAAGATCTGTCTCT11.0No Hit
CGCCCCGCCACCACCTTTCCCGAAGATGCACACCCCCAGGAAC11.0No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG11.0No Hit
GTATCAACGCAGAGTACATGGGAACGTTCAACACAAAAAAAAA11.0No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAAAAT11.0TruSeq Adapter, Index 25 (96% over 27bp)
GACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGC11.0No Hit
GGCAAGATCCAGAAACTGAAGGACAAAGACTTTGGAAAGCAGG11.0No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG11.0No Hit
CTTCAAGCGCCTGCTGGCGCTGGTGCAGGCGCGCAGCCGCGCG11.0No Hit
GGCCAAAGCTTGCTCCCTGCTCCCACACAGGCTCAATAAAGCC11.0No Hit
GTCTTTGATAATGTTGCTGTAATTTTTTAAAATAAAACACGAA11.0No Hit
GTCCCTGCTGCTTGGCAATCTCTGTAGCTTGGATACGGTACTT11.0No Hit
GTTGTCCTCCGTTTCAGGTTAGAATGAGGAGGTCTGCGGCTAG11.0No Hit
ATCCTGGCCAACATGGTGAAACCCCGTCTCTACTCTGTCTCTT11.0No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACCTGTCT11.0No Hit
GGACAATGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGAT11.0No Hit
ATATAACGTTTGAATATATCTGCAGTCCTAAGATTATTTTAAA11.0No Hit
GGAGGGGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCT11.0No Hit
ATCTAGGACCTCGGCAGGCGGTGCGACCAAGCGTTCGGGCCGA11.0No Hit
CGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGG11.0No Hit
GGGTAGGAGGCTTCACACTTAGCCTGGCTCAGCACAGGAGCTG11.0No Hit
TCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT11.0No Hit
TTCCTAAATAGAGCTTTTTTCTTTTTTTTTGAAACGGAATTTC11.0No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATA11.0No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCTGTCT11.0No Hit
GGGGAATTAAGCGAATATTCTCTTCCAGGGTGAGTCCTCACAC11.0No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT11.0No Hit
TGTCCACCATCTCTCTGCCCACCGCCCCTCCAGCCACTGGCAC11.0No Hit
GTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCC11.0No Hit
GGCTTGCAACATTAAACATGAGCTGAGCATCCATAAGCATTGA11.0No Hit
GCTAAATACTTTGACGCCGGTGGGGATAGCGATGATTATGGTA11.0No Hit
ATCCTGGCCAGGGCGCGGCGGTCGTCCAGCGCGCCTCGGCCAC11.0No Hit
CCCTGCTTCCTTTGCTTGCATCCCACAGACTATTTCCCTCATC11.0No Hit
TTTTTGTTTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTA11.0No Hit
TCTCAGATGCCTACAGCACGGGAAGCAGCCTGATGCCCCAGAA11.0No Hit
TGACTTGGCAGTATGTGAATATCCTCTACACTTAGTTATTGAT11.0No Hit
TTATAAGATTTTTCAGAATCAATAGTACGCTCATACAAACAAA11.0No Hit
CCTAGGTAGAGATGAAACAGTTCTGTATCTTGATTCAGGTGCT11.0No Hit
CCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTCTG11.0No Hit
GAGGAAAAGCAGGTGTGAAACGATCTGCAGCGGAGATGTACGG11.0No Hit
TATTTTATCTGTTCAATTCATAGAGTTTTAGAGCTGTCTCTTA11.0No Hit
TGCCAGTAGCATTGCTTGTCTCAAAGATTAAGCCATGCATGTC11.0No Hit
GCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTA11.0No Hit
GACACCAGGTTGCCCGAATTCATGTTCTGGCAATAGAGATCCT11.0No Hit
ATCATATGTTGTTCCTAGGAAGATTGTAGTGGTGAGGGTGTTT11.0No Hit
GGCTCACACAGTAGCCAGGATTAACACTGCTTGGGGCTCCAAT11.0No Hit
GATGAAATCCCAGGTGATGACACATCTCCACGTGATTGAAGAA11.0No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGT11.0No Hit
GGACATACTGCTCCACATCCCGGCGCCCGTGATAGGCCACCAT11.0No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0No Hit
GACAGAACGTGCAGGTTTTTGAGTTCCAGTTGACTGCAGAGGA11.0No Hit
GTACATGGGAATTCCTCGTTCATGGGGAATAATTGCTGTCTCT11.0No Hit
GGAGATTCCCAGTTCATTTAAAGTTGGTCTAAGTTCCTGGATG11.0No Hit
GTACAGAGAAGCACCTATTGACAAAAAGGGGAATTTCAATTAC11.0No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11.0No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCCTGTC11.0No Hit
CCTTTACTTCCCTAAATCTTTCTTATGTACTCCTTTCTTCTGA11.0No Hit
CCTCTGCACGGATCCGCTGTCCCAAAGGCTCCAAGGCCTATGG11.0No Hit
CTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAA11.0No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA11.0No Hit
CTCACTCTGTCACTAGGCTGGAGTGCAGTGATGCGATCTCAGC11.0No Hit
CCGCCAGCATGGCAGAAGCTGAGGAAGATTGTCATTCTGATAC11.0No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG11.0No Hit
AGACAGGAGAGGCTGCGCCTTCCCCGCACCGGGACCTTCGCGA11.0No Hit
GTCATTGTCCAGAGTCCGGCTGTTGTATTTGGGGTGGCGGATG11.0No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT11.0No Hit
GACCTCCCGGGAGCGGGGGACCACCAGGTTACCTCCCCCCTGT11.0No Hit
AGAGAGGCCAGGATATCCGCATCCCCACACCTGTCATCCACAC11.0No Hit
GGGCAACTCTGTCTGGGCCTCTTGGCCTTGGCTCTGAGACAGA11.0No Hit
GGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAAT11.0No Hit
GGATGGGGACAGGATTGCCTCTCATTGCTCCTCCCACTGCTCG11.0No Hit
CCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAGTATGCA11.0No Hit
ATCTTAGCAACTTGGTTAGTAATCTGAAAAGTCTATTAATGTA11.0No Hit
GTTAGGAGAGTTGGTATGCTGAAGAGAGATGGGCATCTGTCTC11.0No Hit
ATTTCTGTATGTCTCCATCTATTGATGAGGGTCTTAAAAAAAA11.0No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG11.0No Hit
ATTATTTCTGTATAACTCGGGACGCGTCAGGCGCGGCGAGCCC11.0No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT11.0No Hit
GTTAGAGACCCTCACAGCAGACCCTCCCATCACAGGCCAGGAG11.0No Hit
GTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGT11.0No Hit
GTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTT11.0No Hit
CAATGCGACGTTGCGCCCGCTTTAAGCTTCGACGGGGTCAAGT11.0No Hit
GTGCAGGCGGCCATCCTGATGGGGGACAAGTCCGAGAACGTGC11.0No Hit
GATGGTGACAGTCCCCAGTGGTAGTGGAATGTTTGCAATAATA11.0No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG11.0No Hit
GTGACAGAGCGAGACTCTGTTTCAAAAAATAAATAAAATCAAG11.0No Hit
CTACACAGCAGGCCGGCAATAAATGCTACCCCATGTACCTGTC11.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers