FastQCFastQC Report
Fri 10 Feb 2017
ERR1631528.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631528.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615469
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40340.24971076510907977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35970.22265979724773424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30030.1858902894453561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.022.725311
CTTACCG450.003826470320.5555559
CATACCG500.007036085218.55
TAACGGC1451.546141E-1017.86206836
GCAGTCG6850.017.5547459
CTTATAC16250.017.07692337
TTAACGG1554.0199666E-1016.70967935
TCTATAC1801.0913936E-1116.4444453
ACTCTAA3100.016.11290410
TCGGTGA7350.016.10884513
CAGTCGG7400.016.00000210
CTCTATG11700.015.6538471
TATACAC4650.015.51612937
CTATATC1802.0190782E-1015.4166664
AAGACGG4450.015.3820225
TCTATGG11600.015.3103452
CGGTGAT7800.015.17948714
AGTCGGT7950.015.12578711
CACTCTA3450.015.0144939
CTTATTG7550.014.94702128
Produced by FastQC (version 0.11.5)