##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631528.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1615469
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72334907076521	34.0	31.0	34.0	31.0	34.0
2	32.9052454736055	34.0	31.0	34.0	31.0	34.0
3	33.02387046733797	34.0	33.0	34.0	31.0	34.0
4	36.40139674608427	37.0	37.0	37.0	35.0	37.0
5	36.347623507476776	37.0	37.0	37.0	35.0	37.0
6	36.38704797182738	37.0	37.0	37.0	35.0	37.0
7	36.38020104378357	37.0	37.0	37.0	35.0	37.0
8	36.365377484804725	37.0	37.0	37.0	35.0	37.0
9	38.19368369185667	39.0	38.0	39.0	37.0	39.0
10	38.12692660769102	39.0	38.0	39.0	37.0	39.0
11	38.195249181507045	39.0	38.0	39.0	37.0	39.0
12	37.982202691602254	39.0	38.0	39.0	37.0	39.0
13	38.11677970917424	39.0	38.0	39.0	37.0	39.0
14	39.603110923205584	41.0	39.0	41.0	37.0	41.0
15	39.594947349655115	41.0	39.0	41.0	37.0	41.0
16	39.57312582290344	41.0	39.0	41.0	37.0	41.0
17	39.56021749720979	41.0	39.0	41.0	37.0	41.0
18	39.546425836707485	41.0	39.0	41.0	37.0	41.0
19	39.55966100247049	41.0	39.0	41.0	37.0	41.0
20	39.52633136259501	41.0	39.0	41.0	37.0	41.0
21	39.50093502258477	41.0	39.0	41.0	37.0	41.0
22	39.451500462094906	41.0	39.0	41.0	37.0	41.0
23	39.39650343027319	41.0	39.0	41.0	37.0	41.0
24	39.37037727124445	41.0	39.0	41.0	36.0	41.0
25	39.35547014520242	41.0	39.0	41.0	36.0	41.0
26	39.27008441511413	40.0	39.0	41.0	36.0	41.0
27	39.20164918051662	40.0	39.0	41.0	36.0	41.0
28	39.12714140599417	40.0	39.0	41.0	36.0	41.0
29	39.05619544540935	40.0	39.0	41.0	35.0	41.0
30	39.00054535246421	40.0	39.0	41.0	35.0	41.0
31	38.946432274466424	40.0	39.0	41.0	35.0	41.0
32	38.87115568296266	40.0	38.0	41.0	35.0	41.0
33	38.773294318863435	40.0	38.0	41.0	35.0	41.0
34	38.73850875504265	40.0	38.0	41.0	35.0	41.0
35	38.64223826022041	40.0	38.0	41.0	35.0	41.0
36	38.590304735033605	40.0	38.0	41.0	35.0	41.0
37	38.525075380586074	40.0	38.0	41.0	35.0	41.0
38	38.43545496694768	40.0	38.0	41.0	35.0	41.0
39	38.35738166439591	40.0	38.0	41.0	34.0	41.0
40	38.26609300457019	40.0	38.0	41.0	34.0	41.0
41	38.17354031553685	40.0	37.0	41.0	34.0	41.0
42	38.05425792757397	40.0	37.0	41.0	34.0	41.0
43	37.14472948722631	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	3.0
13	2.0
14	3.0
15	2.0
16	9.0
17	14.0
18	30.0
19	54.0
20	106.0
21	238.0
22	428.0
23	716.0
24	1125.0
25	1770.0
26	2530.0
27	3590.0
28	5150.0
29	7323.0
30	10204.0
31	13484.0
32	17882.0
33	24342.0
34	36132.0
35	53770.0
36	86819.0
37	168760.0
38	405911.0
39	775065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.103643585856496	18.00108822886728	12.094753907379218	26.80051427789701
2	19.690504738871496	19.586943482047626	33.50395457913461	27.21859719994627
3	20.056899884801254	21.766929603724986	28.501258767577713	29.674911743896043
4	14.880136975701793	15.603084924563703	33.70860103165087	35.80817706808364
5	16.178459629989806	34.73424745383539	32.70777712230937	16.379515793865433
6	34.706886978332605	34.021575158669094	15.813612022267218	15.457925840731082
7	31.08769032398641	28.88368640933376	20.511999920766044	19.516623345913786
8	28.659850483048576	31.59454003759899	19.1517757381912	20.593833741161234
9	27.607895911342155	14.08222627608453	19.10039746971313	39.20948034286019
10	18.84802493888772	24.904470466471345	30.88062971186696	25.36687488277398
11	37.55862848497867	21.111082911526	20.358484130614702	20.97180447288063
12	23.340837861945975	24.698771687974205	27.085199406488147	24.875191043591673
13	31.230992361970422	18.320933425525343	23.76746319489882	26.680611017605415
14	23.808132498983266	19.69929475588823	23.65839270205742	32.83418004307108
15	27.000765721904905	26.11408823072433	20.750197001613774	26.13494904575699
16	26.66334049121339	24.573978206947952	22.90746526240986	25.855216039428797
17	25.24672401636924	25.22165389741307	23.871705368533842	25.659916717683846
18	25.396711419408234	23.552045876460646	25.06758099350715	25.983661710623974
19	25.88486687147819	24.21253518328114	24.91400330182752	24.98859464341315
20	25.906903815548304	23.69126241357773	24.682120176865048	25.719713594008926
21	26.97334334487384	23.85579667576413	24.18325576040147	24.987604218960563
22	27.093246605165433	23.360398744884613	23.93174985097207	25.61460479897788
23	25.731970096609714	23.548579390876583	24.528604386713702	26.1908461258
24	25.359756207020993	24.154657254332953	24.18628893528752	26.29929760335853
25	26.03114018281997	23.246685637421702	24.441632739470705	26.28054144028762
26	25.768058687600938	24.2592708371377	24.525756916412508	25.446913558848856
27	26.028540318631926	23.641741190948263	24.260880276873156	26.068838213546652
28	25.307882726316627	23.812156098321914	25.153809822410704	25.726151352950755
29	25.11561657945773	23.91868862850355	25.385569144316605	25.58012564772212
30	24.71486608532878	24.56091698447943	25.1077550853653	25.61646184482649
31	25.736798415816086	24.104455114892332	24.274374810039685	25.884371659251897
32	25.031740008629072	23.83035514763824	24.68812462510887	26.44978021862382
33	24.49777742562686	23.61407120780405	25.360375222303865	26.527776144265225
34	25.969857669816008	23.08648448221538	24.967610025323918	25.976047822644695
35	25.498291827326923	23.392401835008904	25.65719305043922	25.452113287224947
36	24.690167375542334	23.800023398777693	25.108807411346177	26.401001814333796
37	25.155790671315888	22.52268536257892	25.97289084470206	26.348633121403136
38	24.21234947869628	22.45886488691519	26.834498216926477	26.494287417462047
39	24.271032127512196	22.146943085877847	27.238653295111202	26.34337149149875
40	24.02614968160949	22.076127737517712	27.823932245063197	26.073790335809598
41	23.04897215607356	22.240166787477815	28.491106916938673	26.21975413950995
42	23.347399423944378	22.38582108353673	28.52552416666615	25.74125532585274
43	22.529742136803616	21.700756869986364	28.850754796285166	26.918746196924854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	139.5
2	150.0
3	339.0
4	528.0
5	528.0
6	675.0
7	822.0
8	759.0
9	696.0
10	1113.5
11	1531.0
12	1531.0
13	2669.5
14	3808.0
15	5828.0
16	7848.0
17	7323.0
18	6798.0
19	6798.0
20	7634.5
21	8471.0
22	7185.0
23	5899.0
24	6833.5
25	7768.0
26	7768.0
27	9448.0
28	11128.0
29	15186.5
30	19245.0
31	23539.5
32	27834.0
33	27834.0
34	34897.5
35	41961.0
36	50562.5
37	59164.0
38	67181.0
39	75198.0
40	75198.0
41	82498.5
42	89799.0
43	96626.5
44	103454.0
45	109100.0
46	114746.0
47	114746.0
48	120612.5
49	126479.0
50	127341.0
51	128203.0
52	127665.0
53	127127.0
54	127127.0
55	121941.0
56	116755.0
57	110279.0
58	103803.0
59	101076.5
60	98350.0
61	98350.0
62	91808.5
63	85267.0
64	77714.0
65	70161.0
66	62512.0
67	54863.0
68	54863.0
69	46967.0
70	39071.0
71	34063.5
72	29056.0
73	23006.5
74	16957.0
75	16957.0
76	14015.0
77	11073.0
78	9289.0
79	7505.0
80	6536.5
81	5568.0
82	5568.0
83	4466.0
84	3364.0
85	2860.5
86	2357.0
87	1956.5
88	1556.0
89	1556.0
90	1170.5
91	785.0
92	456.0
93	127.0
94	88.0
95	49.0
96	49.0
97	30.5
98	12.0
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1615469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.94438578353182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.62079208458466	45.331402472178176
2	7.697225967640055	8.150498021875082
3	2.326614814293399	3.6954357689288986
4	1.072327386328089	2.270948593120029
5	0.6331470730108024	1.6760791445598955
6	0.4076863287570304	1.2950821361030402
7	0.29147966170328243	1.0802548160090353
8	0.2358817417242259	0.9990891146511065
9	0.17901569183506083	0.8530088264839176
>10	1.2426408237378277	13.269404945002975
>50	0.16598815063237632	6.128166430260371
>100	0.1194485457607545	11.774825191552885
>500	0.006694675902600545	2.3364798869096393
>1k	0.0010570540898842966	1.1393246523648908
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4034	0.24971076510907977	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3597	0.22265979724773424	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3003	0.1858902894453561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.190152828683187E-5	0.0
2	0.0	0.0	0.0	6.190152828683187E-5	0.0
3	0.0	0.0	0.0	6.190152828683187E-5	0.0
4	0.0	0.0	0.0	6.190152828683187E-5	0.0
5	0.0	0.0	0.0	6.190152828683187E-5	0.0
6	0.0	0.0	0.0	6.190152828683187E-5	6.190152828683187E-5
7	0.0	0.0	0.0	6.190152828683187E-5	6.190152828683187E-5
8	0.0	0.0	0.0	6.190152828683187E-5	1.8570458486049561E-4
9	0.0	0.0	0.0	6.190152828683187E-5	1.8570458486049561E-4
10	0.0	0.0	0.0	1.2380305657366374E-4	1.8570458486049561E-4
11	0.0	0.0	0.0	1.8570458486049561E-4	1.8570458486049561E-4
12	0.0	0.0	0.0	3.0950764143415936E-4	1.8570458486049561E-4
13	0.0	0.0	0.0	3.0950764143415936E-4	1.8570458486049561E-4
14	0.0	0.0	0.0	3.7140916972099123E-4	1.8570458486049561E-4
15	0.0	0.0	0.0	4.95212226294655E-4	1.8570458486049561E-4
16	0.0	0.0	0.0	8.666213960156462E-4	3.0950764143415936E-4
17	0.0	0.0	0.0	9.9042445258931E-4	3.0950764143415936E-4
18	0.0	0.0	0.0	0.0011761290374498056	4.333106980078231E-4
19	0.0	0.0	0.0	0.0012380305657366374	4.333106980078231E-4
20	0.0	0.0	0.0	0.0012380305657366374	5.571137545814868E-4
21	0.0	0.0	0.0	0.0012999320940234693	6.190152828683187E-4
22	0.0	0.0	0.0	0.0015475382071707968	6.190152828683187E-4
23	0.0	0.0	0.0	0.002414159603186443	6.190152828683187E-4
24	0.0	0.0	0.0	0.004147402395217736	6.190152828683187E-4
25	0.0	0.0	0.0	0.005633039074101701	6.190152828683187E-4
26	0.0	0.0	0.0	0.011513684261350729	6.809168111551506E-4
27	0.0	0.0	0.0	0.03646000016094397	7.428183394419825E-4
28	0.0	0.0	0.0	0.12262692753621394	8.047198677288143E-4
29	0.0	0.0	0.0	0.24153976337521796	8.047198677288143E-4
30	0.0	0.0	0.0	0.3977792207711816	8.047198677288143E-4
31	0.0	0.0	0.0	0.856036234678598	8.047198677288143E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1620	0.0	22.72531	1
CTTACCG	45	0.0038264703	20.555555	9
CATACCG	50	0.0070360852	18.5	5
TAACGGC	145	1.546141E-10	17.862068	36
GCAGTCG	685	0.0	17.554745	9
CTTATAC	1625	0.0	17.076923	37
TTAACGG	155	4.0199666E-10	16.709679	35
TCTATAC	180	1.0913936E-11	16.444445	3
ACTCTAA	310	0.0	16.112904	10
TCGGTGA	735	0.0	16.108845	13
CAGTCGG	740	0.0	16.000002	10
CTCTATG	1170	0.0	15.653847	1
TATACAC	465	0.0	15.516129	37
CTATATC	180	2.0190782E-10	15.416666	4
AAGACGG	445	0.0	15.382022	5
TCTATGG	1160	0.0	15.310345	2
CGGTGAT	780	0.0	15.179487	14
AGTCGGT	795	0.0	15.125787	11
CACTCTA	345	0.0	15.014493	9
CTTATTG	755	0.0	14.947021	28
>>END_MODULE