FastQCFastQC Report
Fri 10 Feb 2017
ERR1631527.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631527.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1628
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70.42997542997543No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT40.2457002457002457TruSeq Adapter, Index 6 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC40.2457002457002457No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG30.18427518427518427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30.18427518427518427No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC30.18427518427518427RNA PCR Primer, Index 6 (95% over 22bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT30.18427518427518427No Hit
GTCTCCTCCTGAGACTCAGCGAAGTTGAAATGTTTGTGATGAT30.18427518427518427No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA30.18427518427518427No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTCGGCAA20.12285012285012285No Hit
CAAGGACATCGCCTGCGTGCTGGTCAATCCGCTGCAGGCGCTG20.12285012285012285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20.12285012285012285No Hit
TATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCTGTC20.12285012285012285No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTC20.12285012285012285No Hit
CTGCAACCTCTGCCTCCCCATGTACTCTGCGTTGATACTGTCT20.12285012285012285No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT20.12285012285012285No Hit
GTATCAACGCAGAGTACATGGGTGCAGAAAGGAGAGAGTCTGT20.12285012285012285No Hit
AGGTGGCAGTGAGCTGAGATTGTGCCACTGCACTCCTGTCTCT20.12285012285012285No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC20.12285012285012285No Hit
GTGCTTGGTCACGCCGCCTTCGATCTCGGAGGTGATCTTGTTG20.12285012285012285No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12285012285012285No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.12285012285012285No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGT20.12285012285012285No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT20.12285012285012285No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA20.12285012285012285No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT20.12285012285012285No Hit
GGTATCAACGCAGAGTACATGGGGGAGGTGGAGGGTGGAGGTT20.12285012285012285No Hit
AACGCAGAGTACATGGGCCGCCCCACAATCCTGTCTCTGTCTC20.12285012285012285No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTT20.12285012285012285No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATAACGCCTGTC20.12285012285012285No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT20.12285012285012285No Hit
GGGTTTCACCGTGCCATGTACTCTGCGTTGATACCTGTCTCTT20.12285012285012285No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC20.12285012285012285No Hit
GGGGCAGAGTCTTGCTCTGTGGCCCAGGCTGGAGTCTGTCTCT20.12285012285012285No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC20.12285012285012285No Hit
CGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCTCT20.12285012285012285No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)