##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631527.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1628
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83353808353808	34.0	33.0	34.0	31.0	34.0
2	32.964373464373466	34.0	33.0	34.0	31.0	34.0
3	33.06572481572481	34.0	33.0	34.0	31.0	34.0
4	36.36916461916462	37.0	37.0	37.0	35.0	37.0
5	36.37960687960688	37.0	37.0	37.0	35.0	37.0
6	36.52395577395578	37.0	37.0	37.0	35.0	37.0
7	36.50429975429975	37.0	37.0	37.0	35.0	37.0
8	36.42813267813268	37.0	37.0	37.0	35.0	37.0
9	38.264127764127764	39.0	39.0	39.0	37.0	39.0
10	38.25	39.0	39.0	39.0	37.0	39.0
11	38.30773955773956	39.0	39.0	39.0	37.0	39.0
12	38.11486486486486	39.0	39.0	39.0	37.0	39.0
13	38.235872235872236	39.0	39.0	39.0	37.0	39.0
14	39.67874692874693	41.0	39.0	41.0	37.0	41.0
15	39.69410319410319	41.0	39.0	41.0	38.0	41.0
16	39.75798525798526	41.0	40.0	41.0	38.0	41.0
17	39.67076167076167	41.0	40.0	41.0	37.0	41.0
18	39.73525798525799	41.0	40.0	41.0	38.0	41.0
19	39.70945945945946	41.0	40.0	41.0	38.0	41.0
20	39.78255528255528	41.0	40.0	41.0	38.0	41.0
21	39.70085995085995	41.0	40.0	41.0	38.0	41.0
22	39.75	41.0	40.0	41.0	37.0	41.0
23	39.65970515970516	41.0	40.0	41.0	37.0	41.0
24	39.53869778869779	41.0	39.0	41.0	37.0	41.0
25	39.466830466830466	41.0	39.0	41.0	37.0	41.0
26	39.503071253071255	41.0	39.0	41.0	37.0	41.0
27	39.291769041769044	41.0	39.0	41.0	37.0	41.0
28	39.37223587223587	41.0	39.0	41.0	36.0	41.0
29	39.257371007371006	41.0	39.0	41.0	36.0	41.0
30	39.31879606879607	41.0	39.0	41.0	36.0	41.0
31	39.216830466830466	41.0	39.0	41.0	35.0	41.0
32	39.13452088452088	40.0	39.0	41.0	35.0	41.0
33	39.19471744471745	40.0	39.0	41.0	35.0	41.0
34	39.17997542997543	40.0	39.0	41.0	35.0	41.0
35	38.92567567567568	40.0	39.0	41.0	35.0	41.0
36	38.917690417690416	40.0	38.0	41.0	35.0	41.0
37	38.83353808353808	40.0	39.0	41.0	35.0	41.0
38	38.8009828009828	40.0	39.0	41.0	35.0	41.0
39	38.71990171990172	40.0	38.0	41.0	35.0	41.0
40	38.6990171990172	40.0	38.0	41.0	35.0	41.0
41	38.66154791154791	40.0	38.0	41.0	35.0	41.0
42	38.49938574938575	40.0	38.0	41.0	35.0	41.0
43	37.45884520884521	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	0.0
25	2.0
26	3.0
27	5.0
28	6.0
29	6.0
30	10.0
31	9.0
32	16.0
33	13.0
34	31.0
35	49.0
36	57.0
37	153.0
38	377.0
39	890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.533169533169534	19.226044226044227	13.022113022113022	23.21867321867322
2	19.84029484029484	22.420147420147423	34.21375921375922	23.525798525798525
3	21.4987714987715	21.683046683046683	30.282555282555286	26.535626535626534
4	14.987714987714988	16.584766584766587	32.432432432432435	35.995085995085994
5	15.847665847665848	35.810810810810814	31.32678132678133	17.014742014742016
6	33.47665847665848	35.13513513513514	14.312039312039312	17.076167076167074
7	27.27272727272727	31.511056511056513	17.874692874692872	23.34152334152334
8	31.756756756756754	28.37837837837838	20.208845208845208	19.656019656019655
9	27.457002457002456	11.547911547911548	18.488943488943487	42.50614250614251
10	19.103194103194106	24.324324324324326	28.93120393120393	27.64127764127764
11	36.977886977886975	21.19164619164619	17.56756756756757	24.26289926289926
12	23.894348894348894	29.545454545454547	24.447174447174447	22.11302211302211
13	34.64373464373464	17.936117936117938	21.437346437346438	25.982800982800985
14	23.771498771498774	20.884520884520885	23.587223587223587	31.756756756756754
15	30.22113022113022	24.877149877149876	18.91891891891892	25.982800982800985
16	27.14987714987715	24.26289926289926	20.14742014742015	28.43980343980344
17	22.78869778869779	27.395577395577398	22.11302211302211	27.7027027027027
18	23.464373464373462	22.174447174447174	24.938574938574938	29.42260442260442
19	26.351351351351347	24.938574938574938	25.552825552825553	23.157248157248155
20	31.57248157248157	20.208845208845208	25.49140049140049	22.727272727272727
21	33.47665847665848	20.14742014742015	21.4987714987715	24.877149877149876
22	32.30958230958231	20.700245700245702	22.604422604422606	24.385749385749385
23	29.484029484029485	22.11302211302211	20.884520884520885	27.51842751842752
24	28.194103194103192	23.095823095823096	25.24570024570025	23.464373464373462
25	25.73710073710074	23.402948402948404	23.157248157248155	27.7027027027027
26	26.78132678132678	22.727272727272727	24.14004914004914	26.351351351351347
27	25.307125307125304	22.358722358722357	24.01719901719902	28.31695331695332
28	25.184275184275183	23.894348894348894	25.675675675675674	25.24570024570025
29	22.91154791154791	22.11302211302211	26.105651105651106	28.869778869778866
30	26.289926289926292	23.095823095823096	25.061425061425062	25.552825552825553
31	26.412776412776413	25.36855036855037	21.56019656019656	26.65847665847666
32	22.48157248157248	22.48157248157248	28.992628992628994	26.044226044226043
33	25.0	20.515970515970515	28.255528255528255	26.228501228501226
34	25.675675675675674	21.314496314496314	26.65847665847666	26.351351351351347
35	24.385749385749385	21.437346437346438	27.27272727272727	26.9041769041769
36	22.604422604422606	23.34152334152334	26.16707616707617	27.88697788697789
37	22.420147420147423	23.34152334152334	27.94840294840295	26.289926289926292
38	23.955773955773957	20.700245700245702	26.78132678132678	28.562653562653562
39	21.4987714987715	19.28746928746929	31.511056511056513	27.7027027027027
40	19.656019656019655	18.243243243243242	32.55528255528255	29.545454545454547
41	21.56019656019656	18.42751842751843	33.845208845208845	26.16707616707617
42	19.84029484029484	19.164619164619165	34.76658476658477	26.228501228501226
43	16.646191646191646	18.98034398034398	36.85503685503686	27.51842751842752
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	0.5
11	1.0
12	1.0
13	2.0
14	3.0
15	6.0
16	9.0
17	7.5
18	6.0
19	6.0
20	7.0
21	8.0
22	8.0
23	8.0
24	8.0
25	8.0
26	8.0
27	12.5
28	17.0
29	13.5
30	10.0
31	16.0
32	22.0
33	22.0
34	26.5
35	31.0
36	35.5
37	40.0
38	47.5
39	55.0
40	55.0
41	63.5
42	72.0
43	80.5
44	89.0
45	98.0
46	107.0
47	107.0
48	125.5
49	144.0
50	150.5
51	157.0
52	148.5
53	140.0
54	140.0
55	142.5
56	145.0
57	160.5
58	176.0
59	139.5
60	103.0
61	103.0
62	92.5
63	82.0
64	74.0
65	66.0
66	57.0
67	48.0
68	48.0
69	37.0
70	26.0
71	22.5
72	19.0
73	17.0
74	15.0
75	15.0
76	14.0
77	13.0
78	9.5
79	6.0
80	3.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1628.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.86732186732186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.71718452758402	94.65601965601965
2	1.7121116043119846	3.3169533169533167
3	0.3804692454026633	1.1056511056511056
4	0.12682308180088775	0.4914004914004914
5	0.0	0.0
6	0.0	0.0
7	0.06341154090044387	0.42997542997543
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.42997542997543	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	4	0.2457002457002457	TruSeq Adapter, Index 6 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	4	0.2457002457002457	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	3	0.18427518427518427	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.18427518427518427	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	3	0.18427518427518427	RNA PCR Primer, Index 6 (95% over 22bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	3	0.18427518427518427	No Hit
GTCTCCTCCTGAGACTCAGCGAAGTTGAAATGTTTGTGATGAT	3	0.18427518427518427	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	3	0.18427518427518427	No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTCGGCAA	2	0.12285012285012285	No Hit
CAAGGACATCGCCTGCGTGCTGGTCAATCCGCTGCAGGCGCTG	2	0.12285012285012285	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.12285012285012285	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCTGTC	2	0.12285012285012285	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTC	2	0.12285012285012285	No Hit
CTGCAACCTCTGCCTCCCCATGTACTCTGCGTTGATACTGTCT	2	0.12285012285012285	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	2	0.12285012285012285	No Hit
GTATCAACGCAGAGTACATGGGTGCAGAAAGGAGAGAGTCTGT	2	0.12285012285012285	No Hit
AGGTGGCAGTGAGCTGAGATTGTGCCACTGCACTCCTGTCTCT	2	0.12285012285012285	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	2	0.12285012285012285	No Hit
GTGCTTGGTCACGCCGCCTTCGATCTCGGAGGTGATCTTGTTG	2	0.12285012285012285	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.12285012285012285	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.12285012285012285	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGT	2	0.12285012285012285	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	2	0.12285012285012285	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	2	0.12285012285012285	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	2	0.12285012285012285	No Hit
GGTATCAACGCAGAGTACATGGGGGAGGTGGAGGGTGGAGGTT	2	0.12285012285012285	No Hit
AACGCAGAGTACATGGGCCGCCCCACAATCCTGTCTCTGTCTC	2	0.12285012285012285	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTT	2	0.12285012285012285	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATAACGCCTGTC	2	0.12285012285012285	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGCTGTCTCTT	2	0.12285012285012285	No Hit
GGGTTTCACCGTGCCATGTACTCTGCGTTGATACCTGTCTCTT	2	0.12285012285012285	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	2	0.12285012285012285	No Hit
GGGGCAGAGTCTTGCTCTGTGGCCCAGGCTGGAGTCTGTCTCT	2	0.12285012285012285	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC	2	0.12285012285012285	No Hit
CGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCTCT	2	0.12285012285012285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.06142506142506143	0.0
28	0.0	0.0	0.0	0.06142506142506143	0.0
29	0.0	0.0	0.0	0.6142506142506142	0.0
30	0.0	0.0	0.0	0.9213759213759214	0.0
31	0.0	0.0	0.0	2.272727272727273	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE