FastQCFastQC Report
Fri 10 Feb 2017
ERR1631526.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631526.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21370
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5572.6064576509124944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5382.517547964436125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3911.8296677585400094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2241.048198408984558No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1530.7159569489939167No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.5147402901263454No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA740.34627983153954145No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.32756200280767434No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.3182030884417408No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA630.2948058025269069No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA610.28544688816097336No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.271408516612073No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.2620496022461395No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA520.24333177351427235No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG510.23865231633130557No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA500.2339728591483388No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC470.21993448759943846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.1965372016846046No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.1918577445016378No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT410.1918577445016378No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT400.18717828731867103No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC400.18717828731867103No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT390.18249883013570425No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.17781937295273748No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA350.16378100140383717No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT340.1591015442208704No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT340.1591015442208704No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA340.1591015442208704No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.1591015442208704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC310.14506317267197005No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT310.14506317267197005No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT310.14506317267197005No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT280.13102480112306975No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC270.12634534394010297No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA270.12634534394010297No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.12166588675713617No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA240.11230697239120262No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG230.10762751520823584No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA230.10762751520823584No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC230.10762751520823584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT230.10762751520823584No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA220.10294805802526907ABI Solid3 Adapter B (100% over 21bp)
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA220.10294805802526907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGG1050.029.95238310
AGTCGGT1050.029.95238311
GGCAGTC1050.029.9523838
GGGCAGT1100.028.5909087
GCAGTCG1100.028.5909089
TGGGCAG1100.028.5909086
CTCCGCT405.6757744E-527.7523
GGTGATT1150.027.34782415
CGGTGAT1150.027.34782414
GTGATTC1150.027.34782416
TGATTCC1200.026.20833217
GATTCCT1200.026.20833218
ATTCCTC1200.026.20833219
GTCGGTG1200.026.20833212
TTCCTCC508.558201E-625.90000220
TCGGTGA1250.025.1613
TCCTCCG451.2647887E-424.66666621
CCGCTTA451.2647887E-424.66666625
CCTCCGC451.2647887E-424.66666622
TCCGCTT451.2647887E-424.66666624
Produced by FastQC (version 0.11.5)