##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631526.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21370
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70711277491811	34.0	31.0	34.0	31.0	34.0
2	32.81272812353767	34.0	31.0	34.0	31.0	34.0
3	32.841272812353765	34.0	33.0	34.0	31.0	34.0
4	36.25928872250819	37.0	37.0	37.0	35.0	37.0
5	36.24627983153954	37.0	37.0	37.0	35.0	37.0
6	36.33331773514272	37.0	37.0	37.0	35.0	37.0
7	36.35573233504913	37.0	37.0	37.0	35.0	37.0
8	36.3995320542817	37.0	37.0	37.0	35.0	37.0
9	38.196817969115585	39.0	39.0	39.0	37.0	39.0
10	38.18025269068788	39.0	39.0	39.0	37.0	39.0
11	38.224894712213384	39.0	39.0	39.0	37.0	39.0
12	38.07299953205428	39.0	39.0	39.0	37.0	39.0
13	38.2072531586336	39.0	39.0	39.0	37.0	39.0
14	39.59096864763688	41.0	39.0	41.0	37.0	41.0
15	39.55105287786617	41.0	39.0	41.0	37.0	41.0
16	39.42072999532054	41.0	39.0	41.0	37.0	41.0
17	39.469583528310714	41.0	39.0	41.0	37.0	41.0
18	39.568554047730466	41.0	39.0	41.0	37.0	41.0
19	39.570566214319136	41.0	40.0	41.0	37.0	41.0
20	39.531305568554046	41.0	39.0	41.0	37.0	41.0
21	39.52938699110903	41.0	40.0	41.0	37.0	41.0
22	39.375620028076746	41.0	39.0	41.0	36.0	41.0
23	39.301310248011234	41.0	39.0	41.0	35.0	41.0
24	39.24595226953674	41.0	39.0	41.0	35.0	41.0
25	39.20102948058025	41.0	39.0	41.0	35.0	41.0
26	38.97599438465138	41.0	38.0	41.0	35.0	41.0
27	38.79134300421151	40.0	38.0	41.0	35.0	41.0
28	38.59883013570426	40.0	38.0	41.0	35.0	41.0
29	38.41441272812354	40.0	37.0	41.0	35.0	41.0
30	38.161207299953205	40.0	37.0	41.0	35.0	41.0
31	37.86036499766027	40.0	35.0	41.0	35.0	41.0
32	37.576649508657	40.0	35.0	41.0	34.0	41.0
33	37.29405708937763	40.0	35.0	41.0	33.0	41.0
34	37.07721104351895	39.0	35.0	41.0	33.0	41.0
35	36.74791764155358	39.0	35.0	41.0	33.0	41.0
36	36.50435189518016	39.0	35.0	41.0	33.0	41.0
37	36.22742161909218	39.0	35.0	41.0	31.0	41.0
38	35.91052877866168	39.0	35.0	41.0	30.0	41.0
39	35.480907814693495	39.0	35.0	41.0	27.0	41.0
40	35.11871782873187	38.0	35.0	41.0	23.0	41.0
41	34.65676181562939	38.0	35.0	41.0	18.0	41.0
42	34.209592887225085	38.0	35.0	41.0	15.0	41.0
43	33.20987365465606	36.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	3.0
21	4.0
22	17.0
23	20.0
24	41.0
25	53.0
26	84.0
27	113.0
28	215.0
29	267.0
30	372.0
31	385.0
32	459.0
33	563.0
34	800.0
35	1020.0
36	1518.0
37	3451.0
38	4020.0
39	7961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65278427702387	17.080018717828732	15.680861020121666	21.586335985025737
2	18.614880673841835	22.115114646700984	33.57978474496958	25.6902199344876
3	22.110435189518014	24.41272812353767	28.84885353299017	24.62798315395414
4	14.567150210575575	19.120262049602246	30.060832943378568	36.25175479644361
5	13.102480112306973	39.90641085634066	29.354234908750588	17.63687412260178
6	28.128217126813286	30.33692091717361	20.921853065044456	20.613008890968647
7	28.31539541413196	34.23490875058493	18.816097332709404	18.6335985025737
8	34.06644829199813	29.054749649040712	18.408984557791296	18.469817501169864
9	31.066916237716423	12.723444080486662	20.0701918577445	36.13944782405241
10	22.026204960224614	28.029948525970987	24.48759943846514	25.45624707533926
11	31.375760411792232	26.073935423490873	17.02386523163313	25.52643893308376
12	22.831071595694898	32.18062704726252	25.38605521759476	19.602246139447825
13	33.33177351427234	19.46654188114179	27.473093121197937	19.728591483387927
14	23.50023397285915	22.44267664950866	28.198408984557794	25.8586803930744
15	30.88441740758072	24.67477772578381	23.593823116518482	20.846981750116985
16	22.222742161909217	25.93823116518484	29.42442676649509	22.414599906410857
17	18.100140383715488	31.431913897987833	28.4370613008891	22.03088441740758
18	19.86429574169396	23.565746373420684	33.107159569489944	23.462798315395414
19	20.374356574637343	28.993916705662144	34.122601778193726	16.509124941506787
20	22.241459990641086	23.52831071595695	35.75573233504913	18.47449695835283
21	25.27374824520356	22.522227421619093	32.9012634534394	19.302760879737953
22	23.266261113710808	23.256902199344875	30.66916237716425	22.807674309780065
23	20.215255030416472	25.900795507721103	31.066916237716423	22.817033224145998
24	19.227889564810482	26.284510996724382	34.59990641085634	19.887693027608798
25	18.47449695835283	25.98034627983154	32.751520823584464	22.793635938231166
26	22.52690687880206	24.91810949929808	32.157229761347686	20.397753860552175
27	20.65512400561535	25.194197473093123	30.89845577912962	23.25222274216191
28	18.703790360318205	25.802526906878803	34.24426766495087	21.24941506785213
29	18.235844642021526	26.82732802994853	34.98362189985962	19.95320542817033
30	18.05334581188582	27.65091249415068	36.22835751052878	18.06738418343472
31	17.889564810481986	29.181094992980817	32.94805802526907	19.981282171268134
32	17.45905474964904	28.5072531586336	35.82124473561067	18.212447356106694
33	19.24192793635938	26.069255966307907	35.596630790828264	19.092185306504444
34	19.213851193261583	27.225081890500704	31.403837154890034	22.157229761347683
35	18.05802526906879	28.497894244267663	32.08703790360318	21.357042583060366
36	16.61675245671502	28.067384183434722	33.02292934019654	22.29293401965372
37	17.276555919513335	27.468413664014975	33.827795975666824	21.427234440804867
38	17.95507721104352	24.13664014974263	36.045858680393074	21.862423958820777
39	18.44642021525503	25.058493214787088	35.72765559195133	20.76743097800655
40	17.27187646233037	25.460926532522226	33.70145063172672	23.565746373420684
41	15.053813757604118	26.560598970519422	35.27374824520356	23.111839026672907
42	14.83387927000468	26.228357510528777	34.2395882077679	24.69817501169864
43	13.80439868975199	28.713149274684135	34.74029012634534	22.74216190921853
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	9.5
2	18.0
3	40.5
4	63.0
5	63.0
6	98.5
7	134.0
8	138.0
9	142.0
10	227.5
11	313.0
12	313.0
13	522.0
14	731.0
15	964.5
16	1198.0
17	1057.0
18	916.0
19	916.0
20	1041.5
21	1167.0
22	802.5
23	438.0
24	364.5
25	291.0
26	291.0
27	284.5
28	278.0
29	282.5
30	287.0
31	315.0
32	343.0
33	343.0
34	319.5
35	296.0
36	300.5
37	305.0
38	364.5
39	424.0
40	424.0
41	499.0
42	574.0
43	747.5
44	921.0
45	1115.5
46	1310.0
47	1310.0
48	1767.0
49	2224.0
50	2127.0
51	2030.0
52	1567.5
53	1105.0
54	1105.0
55	959.0
56	813.0
57	825.5
58	838.0
59	774.5
60	711.0
61	711.0
62	717.0
63	723.0
64	700.0
65	677.0
66	646.5
67	616.0
68	616.0
69	562.5
70	509.0
71	443.5
72	378.0
73	318.5
74	259.0
75	259.0
76	209.0
77	159.0
78	129.5
79	100.0
80	68.0
81	36.0
82	36.0
83	32.5
84	29.0
85	18.0
86	7.0
87	6.0
88	5.0
89	5.0
90	3.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	21370.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.93776321946655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.07385283736085	62.78427702386523
2	4.704045614987782	6.485727655591951
3	1.4458322020092316	2.9901731399157696
4	0.9367363562313331	2.5830603649976602
5	0.38012489818083084	1.3102480112306973
6	0.31903339668748304	1.3196069255966307
7	0.18327450448004345	0.8844174075807206
8	0.14254683681781155	0.7861488067384184
9	0.08824327993483573	0.5474964904071128
>10	0.6244909041542221	8.488535329901731
>50	0.06109150149334781	2.587739822180627
>100	0.027151778441487917	4.108563406644829
>500	0.013575889220743959	5.1240056153486195
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	557	2.6064576509124944	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	538	2.517547964436125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	391	1.8296677585400094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	224	1.048198408984558	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	153	0.7159569489939167	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.5147402901263454	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	74	0.34627983153954145	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.32756200280767434	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68	0.3182030884417408	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	63	0.2948058025269069	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	61	0.28544688816097336	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.271408516612073	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56	0.2620496022461395	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	52	0.24333177351427235	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	51	0.23865231633130557	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	50	0.2339728591483388	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	47	0.21993448759943846	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42	0.1965372016846046	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41	0.1918577445016378	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	41	0.1918577445016378	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	40	0.18717828731867103	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	40	0.18717828731867103	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	39	0.18249883013570425	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.17781937295273748	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.16378100140383717	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	34	0.1591015442208704	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	34	0.1591015442208704	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	34	0.1591015442208704	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34	0.1591015442208704	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	31	0.14506317267197005	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	31	0.14506317267197005	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	31	0.14506317267197005	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	28	0.13102480112306975	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	27	0.12634534394010297	No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA	27	0.12634534394010297	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.12166588675713617	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	24	0.11230697239120262	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	23	0.10762751520823584	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	23	0.10762751520823584	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	23	0.10762751520823584	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	23	0.10762751520823584	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	22	0.10294805802526907	ABI Solid3 Adapter B (100% over 21bp)
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	22	0.10294805802526907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.009358914365933552	0.0
21	0.0	0.0	0.0	0.02339728591483388	0.0
22	0.0	0.0	0.0	0.03743565746373421	0.0
23	0.0	0.0	0.0	0.04679457182966776	0.0
24	0.0	0.0	0.0	0.08890968647636874	0.0
25	0.0	0.0	0.0	0.10294805802526907	0.0
26	0.0	0.0	0.0	0.14974262985493683	0.0
27	0.0	0.0	0.0	0.2526906878802059	0.0
28	0.0	0.0	0.0	0.6176883481516144	0.0
29	0.0	0.0	0.0	1.0201216658867571	0.0
30	0.0	0.0	0.0	1.6846045858680394	0.0
31	0.0	0.0	0.0	2.9386991109031353	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTCGG	105	0.0	29.952383	10
AGTCGGT	105	0.0	29.952383	11
GGCAGTC	105	0.0	29.952383	8
GGGCAGT	110	0.0	28.590908	7
GCAGTCG	110	0.0	28.590908	9
TGGGCAG	110	0.0	28.590908	6
CTCCGCT	40	5.6757744E-5	27.75	23
GGTGATT	115	0.0	27.347824	15
CGGTGAT	115	0.0	27.347824	14
GTGATTC	115	0.0	27.347824	16
TGATTCC	120	0.0	26.208332	17
GATTCCT	120	0.0	26.208332	18
ATTCCTC	120	0.0	26.208332	19
GTCGGTG	120	0.0	26.208332	12
TTCCTCC	50	8.558201E-6	25.900002	20
TCGGTGA	125	0.0	25.16	13
TCCTCCG	45	1.2647887E-4	24.666666	21
CCGCTTA	45	1.2647887E-4	24.666666	25
CCTCCGC	45	1.2647887E-4	24.666666	22
TCCGCTT	45	1.2647887E-4	24.666666	24
>>END_MODULE