FastQCFastQC Report
Fri 10 Feb 2017
ERR1631525.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631525.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488300
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8340.17079664140896988No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC7280.14908867499488018No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC5380.11017816915830432No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC5320.10894941634241245No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC5280.10813024779848454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAG352.3855166E-531.7142851
GTATAAC250.005493285629.61
GGTATCA4350.025.0919551
TCTAGAC1051.8189894E-1224.6666683
CTAGACA1002.0008883E-1124.0500014
CCTTAGG400.001929605823.1252
GTTACAC400.001929605823.1253
ATACACA2200.021.86363637
TTAGGCC450.003822703620.5555574
GGCCGTA450.003822703620.5555577
ATACTAC450.003822703620.5555571
ACACTCC450.003822703620.5555578
CTTAGGC450.003822703620.5555573
GCCGTAT450.003822703620.5555578
TGTATGA555.1372725E-420.1818182
TAGGCCG555.1372725E-420.1818185
GTTCTAG1202.382876E-1020.0416661
TCTTACA1202.382876E-1020.0416662
TTACACT1303.274181E-1119.9230754
ATACACT656.891746E-519.9230754
Produced by FastQC (version 0.11.5)