##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631525.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488300
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84949621134548	34.0	31.0	34.0	31.0	34.0
2	33.024407126766334	34.0	33.0	34.0	31.0	34.0
3	33.14330944091747	34.0	33.0	34.0	31.0	34.0
4	36.481976244112225	37.0	37.0	37.0	35.0	37.0
5	36.44774523858284	37.0	37.0	37.0	35.0	37.0
6	36.49058365758755	37.0	37.0	37.0	35.0	37.0
7	36.478382142125746	37.0	37.0	37.0	35.0	37.0
8	36.45801966004505	37.0	37.0	37.0	35.0	37.0
9	38.316401802170795	39.0	39.0	39.0	37.0	39.0
10	38.269569936514436	39.0	39.0	39.0	37.0	39.0
11	38.34115093180422	39.0	39.0	39.0	37.0	39.0
12	38.1308560311284	39.0	39.0	39.0	37.0	39.0
13	38.24831251279951	39.0	39.0	39.0	37.0	39.0
14	39.783020684005734	41.0	40.0	41.0	38.0	41.0
15	39.78886954740938	41.0	40.0	41.0	38.0	41.0
16	39.787319270939996	41.0	40.0	41.0	38.0	41.0
17	39.776405898013515	41.0	40.0	41.0	38.0	41.0
18	39.76364734794184	41.0	40.0	41.0	38.0	41.0
19	39.771609666188816	41.0	40.0	41.0	38.0	41.0
20	39.750210935900064	41.0	40.0	41.0	38.0	41.0
21	39.71786197010035	41.0	40.0	41.0	38.0	41.0
22	39.68201925046078	41.0	40.0	41.0	37.0	41.0
23	39.6318656563588	41.0	40.0	41.0	37.0	41.0
24	39.605664550481265	41.0	40.0	41.0	37.0	41.0
25	39.58236330124923	41.0	40.0	41.0	37.0	41.0
26	39.53148883882859	41.0	39.0	41.0	37.0	41.0
27	39.46807904976449	41.0	39.0	41.0	37.0	41.0
28	39.39826950645095	41.0	39.0	41.0	37.0	41.0
29	39.34526930165882	41.0	39.0	41.0	36.0	41.0
30	39.30108949416343	41.0	39.0	41.0	36.0	41.0
31	39.27242678681139	41.0	39.0	41.0	36.0	41.0
32	39.20692811796027	40.0	39.0	41.0	36.0	41.0
33	39.1347143149703	40.0	39.0	41.0	35.0	41.0
34	39.087538398525496	40.0	39.0	41.0	35.0	41.0
35	39.020620520172024	40.0	39.0	41.0	35.0	41.0
36	38.97533073929961	40.0	39.0	41.0	35.0	41.0
37	38.92528568502969	40.0	39.0	41.0	35.0	41.0
38	38.854611918902314	40.0	38.0	41.0	35.0	41.0
39	38.79885111611714	40.0	38.0	41.0	35.0	41.0
40	38.73720049150113	40.0	38.0	41.0	35.0	41.0
41	38.6689146016793	40.0	38.0	41.0	35.0	41.0
42	38.571458120008195	40.0	38.0	41.0	35.0	41.0
43	37.66259471636289	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	3.0
17	2.0
18	5.0
19	4.0
20	27.0
21	58.0
22	92.0
23	160.0
24	249.0
25	329.0
26	524.0
27	775.0
28	1134.0
29	1583.0
30	2280.0
31	3047.0
32	4203.0
33	5692.0
34	8798.0
35	13390.0
36	22297.0
37	42508.0
38	109871.0
39	271267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.374564816711036	16.69178783534712	11.544132705304117	27.389514642637725
2	20.066967028466106	18.993241859512594	32.74564816711038	28.194142944910915
3	20.635879582224042	19.853368830636903	27.9750153594102	31.535736227728854
4	15.019045668646324	14.418595125947164	32.326643456891254	38.23571574851526
5	16.95125947163629	34.34609870980954	31.871800122875282	16.830841695678885
6	37.16444808519353	32.8988326848249	14.767765717796436	15.168953512185132
7	32.52078640180217	28.295719844357976	18.82633626868728	20.35715748515257
8	29.392381732541473	31.103829612942864	18.542903952488224	20.960884702027442
9	27.764489043620728	13.59410198648372	17.813843948392382	40.827565021503176
10	18.558468154822855	24.447675609256606	30.260290804833094	26.73356543108745
11	39.16055703460987	20.140487405283636	19.38398525496621	21.31497030514028
12	24.95044030309236	23.556420233463037	26.164038500921567	25.32910096252304
13	32.61806266639361	16.99467540446447	23.516076182674585	26.871185746467336
14	24.67601884087651	18.316403850092154	22.208683186565636	34.7988941224657
15	27.45033790702437	25.195166905590828	19.99590415728036	27.358591030104446
16	27.51628097481057	23.832480032766743	21.486176530821215	27.165062461601476
17	26.72987917263977	24.158918697522015	22.2670489453205	26.844153184517715
18	27.41429449109154	22.221380298996518	23.37988941224657	26.98443579766537
19	27.041163219332375	23.18042187180012	22.721073110792545	27.05734179807495
20	27.270325619496212	22.358795822240427	22.802989965185336	27.567888593078028
21	28.697317223018636	22.466516485766945	23.087651034200288	25.74851525701413
22	28.84292443170182	22.10137210731108	21.89350808928937	27.162195371697727
23	27.323776366987506	22.374974400983003	22.810362482080688	27.4908867499488
24	26.61417161580995	22.95474093794798	22.739299610894943	27.69178783534712
25	27.163014540241655	22.29899651853369	22.69956993651444	27.838419004710218
26	27.222609051812412	22.897603932009012	22.754249436821624	27.12553757935695
27	27.551914806471434	22.184312922383782	23.083759983616627	27.18001228752816
28	26.638951464263773	22.388695474093794	23.568502969485973	27.40385009215646
29	26.20888797870162	22.64980544747082	23.56010649191071	27.581200081916858
30	25.35019455252918	23.376407945934876	24.254966209297564	27.018431292238375
31	26.91808314560721	22.5465902109359	22.852344869956994	27.682981773499897
32	26.01638337087856	22.06307597788245	23.646323981159124	28.27421667007987
33	26.096866680319476	21.732541470407536	24.10055293876715	28.070038910505836
34	26.864018021707963	21.44828998566455	24.477575261109973	27.21011673151751
35	25.865041982387876	21.28077001843129	25.274831046487815	27.57935695269302
36	25.00307188203973	21.129223837804627	25.995289780872415	27.872414499283227
37	25.68216260495597	20.198238787630554	26.213188613557236	27.906409993856236
38	24.33770223223428	19.3602293671923	27.965799713291013	28.336268687282406
39	24.895760802785173	19.47491296334221	28.142740118779443	27.486586115093182
40	23.50296948597174	20.361048535736227	28.34282203563383	27.793159942658203
41	22.977268072906	20.270939995904158	29.681957812819988	27.069834118369858
42	23.00122875281589	20.41695678885931	29.470817120622566	27.11099733770223
43	21.143149703051403	20.06205201720254	29.58283841900471	29.21195986074135
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	19.0
2	22.0
3	50.5
4	79.0
5	79.0
6	92.0
7	105.0
8	102.5
9	100.0
10	149.0
11	198.0
12	198.0
13	296.5
14	395.0
15	619.5
16	844.0
17	801.0
18	758.0
19	758.0
20	867.5
21	977.0
22	927.0
23	877.0
24	1063.0
25	1249.0
26	1249.0
27	1571.5
28	1894.0
29	2430.5
30	2967.0
31	3769.5
32	4572.0
33	4572.0
34	6086.5
35	7601.0
36	9652.0
37	11703.0
38	14206.0
39	16709.0
40	16709.0
41	18995.5
42	21282.0
43	23708.0
44	26134.0
45	29439.5
46	32745.0
47	32745.0
48	35452.5
49	38160.0
50	39470.5
51	40781.0
52	42604.5
53	44428.0
54	44428.0
55	43105.5
56	41783.0
57	41048.0
58	40313.0
59	38504.0
60	36695.0
61	36695.0
62	35127.5
63	33560.0
64	30898.0
65	28236.0
66	24520.5
67	20805.0
68	20805.0
69	16981.0
70	13157.0
71	11063.5
72	8970.0
73	6570.0
74	4170.0
75	4170.0
76	3147.5
77	2125.0
78	1707.5
79	1290.0
80	1170.5
81	1051.0
82	1051.0
83	846.5
84	642.0
85	532.5
86	423.0
87	348.0
88	273.0
89	273.0
90	216.0
91	159.0
92	97.0
93	35.0
94	24.0
95	13.0
96	13.0
97	8.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	488300.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.21590836687214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.07455134711927	45.273195288410584
2	7.06161720763967	7.515807484873605
3	2.435659125442999	3.888474384975317
4	1.2880194350249121	2.7417249691614463
5	0.7664389209672667	2.039337169349922
6	0.5148013537422355	1.6437373000733124
7	0.37423597886910787	1.3940715291359602
8	0.2923378196115865	1.2445618096497109
9	0.23271111178624293	1.114553988069268
>10	1.6876715557526363	17.66030875148968
>50	0.18078902758326243	6.62091433420723
>100	0.08923560975612543	8.213699884949108
>500	0.00193150670467804	0.6496131056548412
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	834	0.17079664140896988	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	728	0.14908867499488018	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	538	0.11017816915830432	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	532	0.10894941634241245	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	528	0.10813024779848454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.0479213598197828E-4	0.0
16	0.0	0.0	0.0	4.0958427196395656E-4	0.0
17	0.0	0.0	0.0	8.191685439279131E-4	0.0
18	0.0	0.0	0.0	0.0010239606799098914	0.0
19	0.0	0.0	0.0	0.0012287528158918697	0.0
20	0.0	0.0	0.0	0.0016383370878558263	0.0
21	0.0	0.0	0.0	0.0018431292238378046	0.0
22	0.0	0.0	0.0	0.0038910505836575876	0.0
23	0.0	0.0	0.0	0.006758140487405284	0.0
24	0.0	0.0	0.0	0.012287528158918698	0.0
25	0.0	0.0	0.0	0.015154618062666393	0.0
26	0.0	0.0	0.0	0.02375588777390948	0.0
27	0.0	0.0	0.0	0.08928937128814253	0.0
28	0.0	0.0	0.0	0.35142330534507477	0.0
29	0.0	0.0	0.0	0.7372516895351219	0.0
30	0.0	0.0	0.0	1.2707352037681754	0.0
31	0.0	0.0	0.0	2.567683800942044	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTAG	35	2.3855166E-5	31.714285	1
GTATAAC	25	0.0054932856	29.6	1
GGTATCA	435	0.0	25.091955	1
TCTAGAC	105	1.8189894E-12	24.666668	3
CTAGACA	100	2.0008883E-11	24.050001	4
CCTTAGG	40	0.0019296058	23.125	2
GTTACAC	40	0.0019296058	23.125	3
ATACACA	220	0.0	21.863636	37
TTAGGCC	45	0.0038227036	20.555557	4
GGCCGTA	45	0.0038227036	20.555557	7
ATACTAC	45	0.0038227036	20.555557	1
ACACTCC	45	0.0038227036	20.555557	8
CTTAGGC	45	0.0038227036	20.555557	3
GCCGTAT	45	0.0038227036	20.555557	8
TGTATGA	55	5.1372725E-4	20.181818	2
TAGGCCG	55	5.1372725E-4	20.181818	5
GTTCTAG	120	2.382876E-10	20.041666	1
TCTTACA	120	2.382876E-10	20.041666	2
TTACACT	130	3.274181E-11	19.923075	4
ATACACT	65	6.891746E-5	19.923075	4
>>END_MODULE