FastQCFastQC Report
Fri 10 Feb 2017
ERR1631524.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631524.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831328
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32770.39418857538781327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29060.3495611840332576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24890.29940047730859537No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA21770.2618701643635244No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC19000.22854998267831708No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC18950.22794853535547943No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC17330.2084616420955387No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17060.20521382655221526No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14000.16840525039454943No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC11740.14121983140228647No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC11660.14025751568574618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.1389343315755033No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTA10760.129431463874668No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10600.12750683244158745No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG9890.11896628045729243No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC9840.11836483313445476No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC9780.11764309634704954No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG9460.11379383348088842No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT9460.11379383348088842No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC9420.11331267562261828No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC9320.11210978097694292No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG9220.11090688633126757No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC8940.10753878132337659No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG8440.10152430809499981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.028.406451
AGTACCG450.00382493120.5555555
GTATTAA701.21868245E-418.51
GAATCGG609.233885E-418.522
TTCTAGC500.00703328518.4999982
CTAACAC1002.872548E-718.4999983
GTATCAA23900.018.3451882
TCGGGTC1156.398477E-817.6956537
ACAACGC852.7216474E-517.4117648
TTTCGGA2150.017.20930330
TTTTCGG2150.017.20930329
AGTCGGT2500.017.0211
CCGCTTA1201.0401527E-716.95833225
AGAGGGT1201.0401527E-716.9583326
GTTTTCG2200.016.81818228
GTCTAGA1005.873855E-616.651
GTTCTAT2350.016.53191414
GCAGTCG2600.016.3653859
GGTTCTA2300.016.08695613
CTCTATG4150.016.0481911
Produced by FastQC (version 0.11.5)