##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631524.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831328
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80780389930328	34.0	31.0	34.0	31.0	34.0
2	32.98302234497094	34.0	33.0	34.0	31.0	34.0
3	33.094468128103465	34.0	33.0	34.0	31.0	34.0
4	36.4139822067824	37.0	37.0	37.0	35.0	37.0
5	36.40374076176912	37.0	37.0	37.0	35.0	37.0
6	36.45960318911428	37.0	37.0	37.0	35.0	37.0
7	36.46023952038185	37.0	37.0	37.0	35.0	37.0
8	36.44760551791832	37.0	37.0	37.0	35.0	37.0
9	38.29353636591093	39.0	39.0	39.0	37.0	39.0
10	38.26389824473613	39.0	39.0	39.0	37.0	39.0
11	38.325843710304476	39.0	39.0	39.0	37.0	39.0
12	38.12407617691212	39.0	39.0	39.0	37.0	39.0
13	38.2357180318719	39.0	39.0	39.0	37.0	39.0
14	39.729234429731704	41.0	40.0	41.0	38.0	41.0
15	39.7289445321221	41.0	40.0	41.0	38.0	41.0
16	39.701024144501325	41.0	40.0	41.0	38.0	41.0
17	39.714512202163284	41.0	40.0	41.0	38.0	41.0
18	39.70104820239424	41.0	40.0	41.0	38.0	41.0
19	39.70745121059317	41.0	40.0	41.0	38.0	41.0
20	39.6873893336926	41.0	40.0	41.0	38.0	41.0
21	39.65123152353824	41.0	40.0	41.0	37.0	41.0
22	39.593303726086454	41.0	40.0	41.0	37.0	41.0
23	39.507866930982715	41.0	39.0	41.0	37.0	41.0
24	39.46233616574926	41.0	39.0	41.0	37.0	41.0
25	39.4546051618615	41.0	39.0	41.0	37.0	41.0
26	39.38085689402979	41.0	39.0	41.0	36.0	41.0
27	39.29634151622464	41.0	39.0	41.0	36.0	41.0
28	39.186642336117636	41.0	39.0	41.0	35.0	41.0
29	39.10872363254936	41.0	39.0	41.0	35.0	41.0
30	39.049743302282614	40.0	39.0	41.0	35.0	41.0
31	38.97727852303784	40.0	39.0	41.0	35.0	41.0
32	38.89360637437931	40.0	38.0	41.0	35.0	41.0
33	38.76818295546403	40.0	38.0	41.0	35.0	41.0
34	38.71558277839794	40.0	38.0	41.0	35.0	41.0
35	38.60605200354132	40.0	38.0	41.0	35.0	41.0
36	38.55130345663805	40.0	38.0	41.0	35.0	41.0
37	38.47920435736557	40.0	38.0	41.0	35.0	41.0
38	38.388981244466684	40.0	38.0	41.0	35.0	41.0
39	38.27009074637207	40.0	38.0	41.0	34.0	41.0
40	38.1555126255822	40.0	37.0	41.0	34.0	41.0
41	38.058912968166595	40.0	37.0	41.0	34.0	41.0
42	37.91097497016821	40.0	37.0	41.0	34.0	41.0
43	37.02733457792833	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	2.0
12	0.0
13	0.0
14	5.0
15	2.0
16	6.0
17	10.0
18	20.0
19	59.0
20	79.0
21	144.0
22	211.0
23	421.0
24	661.0
25	895.0
26	1341.0
27	1980.0
28	2827.0
29	3954.0
30	5384.0
31	7021.0
32	9023.0
33	12209.0
34	17709.0
35	26390.0
36	43655.0
37	83483.0
38	178357.0
39	435477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.07947284345048	18.40946052580931	12.541620154740368	24.969446475999845
2	20.54989125832403	20.077995688825588	32.332725470572385	27.039387582277996
3	21.20979926094153	21.77299453404673	28.627930251356865	28.389275953654874
4	14.782372300704417	15.638472420031563	32.84780495785057	36.731350321413444
5	15.857038377150776	35.82075907463721	31.535567188883327	16.786635359328688
6	34.0896733900458	33.5998546903268	15.988755340852226	16.321716578775163
7	29.90047249701682	30.282511836483312	19.370693637168483	20.44632202933138
8	30.016311251395354	29.719075984448978	18.8659590438431	21.39865372031256
9	27.865655914392395	13.615444204934754	19.49856133800377	39.02033854266908
10	19.983809038069207	25.11319238615805	29.18270526194234	25.7202933138304
11	37.7449093498595	21.478886793179104	19.004532507024905	21.771671349936486
12	23.87060221717541	25.56848800954617	25.667365949420685	24.89354382385773
13	31.854454559451867	18.271849378344047	24.693863312675624	25.179832749528465
14	24.55384637591901	20.104218792101314	23.61197986835521	31.72995496362447
15	28.008319219369493	25.976991031217523	20.92411178259363	25.090577966819357
16	26.773908733977443	24.592699872974325	22.85692289926479	25.77646849378344
17	25.94379113899688	25.412231995072943	22.727250856460987	25.916726009469187
18	25.83204222641364	22.30948554601794	25.2719744793872	26.586497748181227
19	25.57149524616036	24.377020863004734	24.635282343431232	25.416201547403674
20	26.31043342699873	22.282300127025675	25.042823049386044	26.364443396589554
21	28.140276762000077	23.145617614226875	24.608217213903536	24.10588840986951
22	28.467343816159207	22.671677123830786	23.73611859578891	25.1248604642211
23	27.064407790908042	22.651107625389738	24.224253243003965	26.06023134069826
24	24.84230051195196	23.935317949112743	24.490333538627354	26.73204800030794
25	26.113399284037108	23.008968782478156	24.511384194926674	26.36624773855807
26	25.434605835482504	23.926777397128447	24.57970861080103	26.05890815658801
27	26.869057700450366	22.660971361484275	24.773134069825627	25.696836868239732
28	25.312151160552755	23.226211555487126	25.266922321875363	26.19471496208476
29	24.709380653604835	23.229098502636745	25.707783209515377	26.35373763424304
30	23.984636629585435	24.27369221294122	26.063840024635283	25.677831132838065
31	25.775265599137764	23.641571076638822	24.198270718657376	26.384892605566034
32	25.356537972978177	23.163059586589167	25.035846260441126	26.44455617999153
33	24.199593902767617	22.90588071134378	25.645112398475696	27.249412987412914
34	25.5809981138612	22.535509449940335	26.004296739674356	25.879195696524114
35	24.86864390469225	22.198338080757534	26.78978694330036	26.143231071249858
36	23.566390161284115	23.01618615035221	26.399447630778706	27.017976057584974
37	25.027907155779666	21.538550367604604	26.924631433080563	26.50891104353516
38	23.582990107394433	20.731167481427306	27.829569075022132	27.85627333615613
39	24.217035875129913	21.33429885676893	28.10587397513376	26.342791292967398
40	22.866666345894764	21.302542438123098	28.79465144924747	27.036139766734667
41	22.18679029215905	21.686746987951807	29.99754609492282	26.12891662496632
42	22.72087551483891	21.34283940875322	30.075373378498014	25.86091169790985
43	20.81260344893953	20.969581200200164	29.485834712652526	28.731980638207784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	207.0
2	192.0
3	430.0
4	668.0
5	668.0
6	757.0
7	846.0
8	775.5
9	705.0
10	1078.0
11	1451.0
12	1451.0
13	2423.5
14	3396.0
15	4826.5
16	6257.0
17	5778.5
18	5300.0
19	5300.0
20	5913.5
21	6527.0
22	5424.5
23	4322.0
24	4803.0
25	5284.0
26	5284.0
27	6179.5
28	7075.0
29	8427.0
30	9779.0
31	11295.5
32	12812.0
33	12812.0
34	15665.5
35	18519.0
36	21526.5
37	24534.0
38	27255.0
39	29976.0
40	29976.0
41	34672.5
42	39369.0
43	44288.5
44	49208.0
45	54039.5
46	58871.0
47	58871.0
48	62407.5
49	65944.0
50	64220.0
51	62496.0
52	64854.0
53	67212.0
54	67212.0
55	63741.5
56	60271.0
57	56785.5
58	53300.0
59	50824.5
60	48349.0
61	48349.0
62	47684.5
63	47020.0
64	45782.5
65	44545.0
66	39838.5
67	35132.0
68	35132.0
69	29472.5
70	23813.0
71	19183.0
72	14553.0
73	10655.5
74	6758.0
75	6758.0
76	5187.0
77	3616.0
78	3324.5
79	3033.0
80	2984.0
81	2935.0
82	2935.0
83	2610.0
84	2285.0
85	1916.5
86	1548.0
87	1377.5
88	1207.0
89	1207.0
90	1210.0
91	1213.0
92	817.0
93	421.0
94	330.0
95	239.0
96	239.0
97	178.0
98	117.0
99	62.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	831328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.00673722545362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.36702770472556	41.83559494443504
2	6.8011418966129655	6.666035475206697
3	2.3434934206902294	3.4454089877203655
4	1.2867775574905498	2.5224307851020167
5	0.7813072300042033	1.9144659056581521
6	0.5658751359255394	1.6639016453232454
7	0.4046147279804485	1.3880193356180366
8	0.3078534997014031	1.2069516451042341
9	0.23813536258407386	1.0503213424421243
>10	1.6233539815844282	14.971305183619629
>50	0.15474836532994413	5.3177733056600385
>100	0.11014703816630347	10.814490864795639
>500	0.012074283825654107	4.170548010179616
>1k	0.0034497953787583167	3.032752569135131
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3277	0.39418857538781327	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2906	0.3495611840332576	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2489	0.29940047730859537	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2177	0.2618701643635244	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1900	0.22854998267831708	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1895	0.22794853535547943	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1733	0.2084616420955387	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1706	0.20521382655221526	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1400	0.16840525039454943	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1174	0.14121983140228647	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1166	0.14025751568574618	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1155	0.1389343315755033	No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTA	1076	0.129431463874668	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	1060	0.12750683244158745	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	989	0.11896628045729243	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	984	0.11836483313445476	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	978	0.11764309634704954	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	946	0.11379383348088842	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	946	0.11379383348088842	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	942	0.11331267562261828	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	932	0.11210978097694292	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	922	0.11090688633126757	No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCC	894	0.10753878132337659	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	844	0.10152430809499981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.2028946456753532E-4	0.0
12	0.0	0.0	0.0	7.217367874052119E-4	0.0
13	0.0	0.0	0.0	7.217367874052119E-4	0.0
14	0.0	0.0	0.0	9.623157165402826E-4	0.0
15	0.0	0.0	0.0	0.0015637630393779591	0.0
16	0.0	0.0	0.0	0.002165210362215636	0.0
17	0.0	0.0	0.0	0.0028869471496208478	0.0
18	0.0	0.0	0.0	0.0034883944724585243	0.0
19	0.0	0.0	0.0	0.004570999653566342	0.0
20	0.0	0.0	0.0	0.005052157511836484	0.0
21	0.0	0.0	0.0	0.006255052157511837	0.0
22	0.0	0.0	0.0	0.009141999307132683	0.0
23	0.0	0.0	0.0	0.012510104315023673	0.0
24	0.0	0.0	0.0	0.019968051118210862	0.0
25	0.0	0.0	0.0	0.025501366488317488	0.0
26	0.0	0.0	0.0	0.042101312598637364	0.0
27	0.0	0.0	0.0	0.10657646560683628	0.0
28	0.0	0.0	0.0	0.30998595019053854	0.0
29	0.0	0.0	0.0	0.5713749566957927	0.0
30	0.0	0.0	0.0	0.9449940336425574	0.0
31	0.0	0.0	0.0	1.764405866276608	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1550	0.0	28.40645	1
AGTACCG	45	0.003824931	20.555555	5
GTATTAA	70	1.21868245E-4	18.5	1
GAATCGG	60	9.233885E-4	18.5	22
TTCTAGC	50	0.007033285	18.499998	2
CTAACAC	100	2.872548E-7	18.499998	3
GTATCAA	2390	0.0	18.345188	2
TCGGGTC	115	6.398477E-8	17.69565	37
ACAACGC	85	2.7216474E-5	17.411764	8
TTTCGGA	215	0.0	17.209303	30
TTTTCGG	215	0.0	17.209303	29
AGTCGGT	250	0.0	17.02	11
CCGCTTA	120	1.0401527E-7	16.958332	25
AGAGGGT	120	1.0401527E-7	16.958332	6
GTTTTCG	220	0.0	16.818182	28
GTCTAGA	100	5.873855E-6	16.65	1
GTTCTAT	235	0.0	16.531914	14
GCAGTCG	260	0.0	16.365385	9
GGTTCTA	230	0.0	16.086956	13
CTCTATG	415	0.0	16.048191	1
>>END_MODULE