FastQCFastQC Report
Fri 10 Feb 2017
ERR1631523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100938
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2510.2486674988606868No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2440.24173254869325725No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1870.1852622401870455No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1680.16643880401830827No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1520.15058748934989796No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT1430.14167112484891714No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1330.1317640531811607No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT1270.12581981018050684No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC1250.12383839584695555No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1180.11690344567952604No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1180.11690344567952604No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC1170.11591273851275039No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT1130.11194990984564782No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG1120.11095920267887219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCT452.570232E-937.04
ATCTCGT452.570232E-937.07
CGTATGC452.570232E-937.011
CTCGTAT452.570232E-937.09
TATGCCG452.570232E-937.013
TCTCGTA452.570232E-937.08
TCGTATG452.570232E-937.010
GATTATA200.00183399137.01
AAATCTC507.192284E-933.35
GCCGTCT507.192284E-933.316
TCTAGCG303.5764585E-430.83333428
CTAGCGG303.5764585E-430.83333429
AGAAATC551.8204446E-830.2727283
GTATGCC551.8204446E-830.27272812
ATGCCGT551.8204446E-830.27272814
CGGCGGA250.00547290829.59999812
TGCTTGA453.960822E-628.77777925
TGAAAAA453.960822E-628.77777929
GCTTGAA453.960822E-628.77777926
GAAAAAA453.960822E-628.77777930