##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631523.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100938
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83564168103192	34.0	31.0	34.0	31.0	34.0
2	33.01104638490955	34.0	33.0	34.0	31.0	34.0
3	33.13237829162456	34.0	33.0	34.0	31.0	34.0
4	36.44806713031762	37.0	37.0	37.0	35.0	37.0
5	36.43192851057085	37.0	37.0	37.0	35.0	37.0
6	36.465899859319585	37.0	37.0	37.0	35.0	37.0
7	36.454219421823296	37.0	37.0	37.0	35.0	37.0
8	36.4385167132299	37.0	37.0	37.0	35.0	37.0
9	38.304404684063485	39.0	39.0	39.0	37.0	39.0
10	38.251461293070996	39.0	39.0	39.0	37.0	39.0
11	38.320057857298536	39.0	39.0	39.0	37.0	39.0
12	38.11948919138481	39.0	39.0	39.0	37.0	39.0
13	38.23525332382255	39.0	39.0	39.0	37.0	39.0
14	39.75280865481781	41.0	40.0	41.0	38.0	41.0
15	39.762141116328834	41.0	40.0	41.0	38.0	41.0
16	39.75752442093166	41.0	40.0	41.0	38.0	41.0
17	39.74004834650974	41.0	40.0	41.0	38.0	41.0
18	39.70795934137787	41.0	40.0	41.0	38.0	41.0
19	39.70980205670808	41.0	40.0	41.0	38.0	41.0
20	39.695456616933164	41.0	40.0	41.0	37.0	41.0
21	39.64590144445105	41.0	40.0	41.0	37.0	41.0
22	39.61195981691731	41.0	40.0	41.0	37.0	41.0
23	39.55268580712913	41.0	39.0	41.0	37.0	41.0
24	39.511462481919594	41.0	39.0	41.0	37.0	41.0
25	39.49660187441796	41.0	39.0	41.0	37.0	41.0
26	39.43920030117498	41.0	39.0	41.0	37.0	41.0
27	39.35274128673047	41.0	39.0	41.0	36.0	41.0
28	39.2466662703838	41.0	39.0	41.0	36.0	41.0
29	39.176207176682716	41.0	39.0	41.0	35.0	41.0
30	39.11258396243238	41.0	39.0	41.0	35.0	41.0
31	39.073946382928135	40.0	39.0	41.0	35.0	41.0
32	39.015960292456754	40.0	39.0	41.0	35.0	41.0
33	38.90740850819315	40.0	38.0	41.0	35.0	41.0
34	38.85772454377935	40.0	38.0	41.0	35.0	41.0
35	38.75329410132953	40.0	38.0	41.0	35.0	41.0
36	38.6918504428461	40.0	38.0	41.0	35.0	41.0
37	38.58359587073253	40.0	38.0	41.0	35.0	41.0
38	38.47862053934098	40.0	38.0	41.0	35.0	41.0
39	38.37453684439953	40.0	38.0	41.0	35.0	41.0
40	38.298648675424516	40.0	37.0	41.0	34.0	41.0
41	38.19710119083001	40.0	37.0	41.0	34.0	41.0
42	38.0754918861083	40.0	37.0	41.0	34.0	41.0
43	37.082119717054034	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	1.0
20	8.0
21	13.0
22	15.0
23	30.0
24	61.0
25	114.0
26	176.0
27	212.0
28	285.0
29	452.0
30	548.0
31	740.0
32	1035.0
33	1403.0
34	2028.0
35	3111.0
36	5343.0
37	9790.0
38	22629.0
39	52941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63432998474311	16.480413719312846	10.898769541698865	27.98648675424518
2	21.69252412371951	18.97204224375359	29.913412193623806	29.42202143890309
3	21.38540490201906	19.39309278963324	27.79329885672393	31.42820345162377
4	15.59868434088252	14.171075313558818	31.43612910895797	38.794111236600685
5	17.84858031663001	33.57704729636014	31.416314965622462	17.158057421387387
6	38.98729913412194	31.521329925300677	14.261229665735403	15.230141274841982
7	33.37494303433791	27.394043868513346	18.536131090372308	20.69488200677644
8	31.144861201925934	28.35304840595217	18.30331490618003	22.198775485941866
9	28.803820166835088	12.347183419524857	16.330816937129722	42.51817947651033
10	19.331668945293153	23.667994214270145	29.216945055380535	27.78339178505617
11	41.788028294596685	18.482632903366422	17.819849808793517	21.90948899324338
12	26.05064495036557	21.69549624521984	24.619073094374766	27.63478571003983
13	34.30719847827379	15.597693633715744	21.428005310190414	28.667102577820046
14	25.449285700132755	17.922883354138182	20.2530266103945	36.37480433533456
15	29.616200043591117	23.729418058610236	18.228021161505083	28.426360736293567
16	30.246289801660424	21.937228793913093	19.616992609324534	28.199488795101946
17	28.550199132140524	21.998652638253187	20.74639877944877	28.704749450157525
18	29.054469080029328	20.067764370207456	22.114565376765935	28.763201172997288
19	29.288275971388376	21.200142661832018	20.70974261427807	28.801838752501535
20	29.065366858863857	20.208444787889597	21.243733777170142	29.482454576076407
21	31.253838990271255	20.644355941270877	20.565099367928827	27.536705700529033
22	30.565297509362182	20.301571261566504	19.913214052190455	29.219917176880855
23	28.703758742990747	20.00237769720026	20.993084863975906	30.300778695833085
24	28.242089203273295	21.322990350512192	20.977233549307496	29.457686896907013
25	28.950444827517885	20.51952683825715	20.94751233430423	29.582515999920744
26	29.56666468525233	21.22887316966851	20.61463472626761	28.58982741881155
27	29.609265093423687	20.397669856743743	20.895004854465117	29.098060195367452
28	28.408528007291604	20.800887673621432	21.42206106718976	29.368523251897205
29	27.314787295171293	21.12385820999029	22.555430065981096	29.005924428857316
30	26.84717351245319	22.067011432760705	22.220571043610928	28.86524401117518
31	29.10202302403456	21.78366918306287	20.288692068398422	28.82561572450415
32	27.795280271057482	21.277417820840515	21.409181874021677	29.518120034080326
33	27.07206403931126	20.98813132814203	21.826269591234222	30.11353504131249
34	28.17571182309933	21.278408528007294	22.060076482593274	28.485803166300105
35	28.315401533614693	20.954447284471655	22.704036140997445	28.026115040916206
36	27.248409914997325	22.569299966315956	21.68757058788563	28.49471953080109
37	28.191563137767737	20.82862747429115	22.796171907507578	28.183637480433532
38	26.91553230696071	20.587885632764667	23.84235867562266	28.654223384651967
39	27.108720204481962	20.41451187857893	23.87802413362658	28.59874378331253
40	26.740177138441418	20.652281598605086	24.030593037310034	28.576948225643466
41	25.886187560680813	20.34615308407141	25.856466345677543	27.91119300957023
42	26.564821969922132	19.839901721849056	26.279498305890748	27.31577800233807
43	24.131645168321146	19.084982860766015	26.73522360260754	30.048148368305295
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.5
4	4.0
5	4.0
6	5.5
7	7.0
8	5.0
9	3.0
10	6.5
11	10.0
12	10.0
13	25.5
14	41.0
15	53.5
16	66.0
17	59.5
18	53.0
19	53.0
20	56.0
21	59.0
22	60.5
23	62.0
24	77.0
25	92.0
26	92.0
27	131.5
28	171.0
29	239.5
30	308.0
31	392.5
32	477.0
33	477.0
34	673.5
35	870.0
36	1205.0
37	1540.0
38	1985.0
39	2430.0
40	2430.0
41	2801.0
42	3172.0
43	3458.5
44	3745.0
45	4431.5
46	5118.0
47	5118.0
48	5777.5
49	6437.0
50	7015.5
51	7594.0
52	8040.5
53	8487.0
54	8487.0
55	8578.5
56	8670.0
57	8722.5
58	8775.0
59	8883.5
60	8992.0
61	8992.0
62	8747.0
63	8502.0
64	8175.5
65	7849.0
66	7002.5
67	6156.0
68	6156.0
69	5238.5
70	4321.0
71	3726.0
72	3131.0
73	2340.5
74	1550.0
75	1550.0
76	1195.5
77	841.0
78	687.0
79	533.0
80	468.5
81	404.0
82	404.0
83	313.5
84	223.0
85	168.5
86	114.0
87	96.0
88	78.0
89	78.0
90	58.5
91	39.0
92	23.0
93	7.0
94	5.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	100938.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.98779448770533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.80268780578436	56.1830034278468
2	6.001114758159411	7.679961956844796
3	1.9786957329534898	3.798371277417821
4	1.0079271691335852	2.579801462283778
5	0.5759583823620487	1.8427153302026988
6	0.4025515575648727	1.5455031801700052
7	0.30191366817365456	1.3523152826487548
8	0.2895274664024277	1.482097921496364
9	0.2105654301108565	1.2126255721333887
>10	1.3113891125286432	15.866175275911946
>50	0.09599306372700811	4.369018605480592
>100	0.021675853099646994	2.0884107075630585
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	251	0.2486674988606868	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	244	0.24173254869325725	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	187	0.1852622401870455	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	168	0.16643880401830827	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	152	0.15058748934989796	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	143	0.14167112484891714	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	133	0.1317640531811607	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	127	0.12581981018050684	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC	125	0.12383839584695555	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	118	0.11690344567952604	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	118	0.11690344567952604	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTCTGTC	117	0.11591273851275039	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	113	0.11194990984564782	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	112	0.11095920267887219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	9.907071667756445E-4	0.0
17	0.0	0.0	0.0	9.907071667756445E-4	0.0
18	0.0	0.0	0.0	9.907071667756445E-4	0.0
19	0.0	0.0	0.0	9.907071667756445E-4	0.0
20	0.0	0.0	0.0	9.907071667756445E-4	0.0
21	0.0	0.0	0.0	9.907071667756445E-4	0.0
22	0.0	0.0	0.0	9.907071667756445E-4	0.0
23	0.0	0.0	0.0	9.907071667756445E-4	0.0
24	0.0	0.0	0.0	0.001981414333551289	0.0
25	0.0	0.0	0.0	0.0029721215003269334	0.0
26	0.0	0.0	0.0	0.007925657334205156	0.0
27	0.0	0.0	0.0	0.018823436168737245	0.0
28	0.0	0.0	0.0	0.07232162317462204	0.0
29	0.0	0.0	0.0	0.1585131466841031	0.0
30	0.0	0.0	0.0	0.27541659236362914	0.0
31	0.0	0.0	0.0	0.48742792605361707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAATCT	45	2.570232E-9	37.0	4
ATCTCGT	45	2.570232E-9	37.0	7
CGTATGC	45	2.570232E-9	37.0	11
CTCGTAT	45	2.570232E-9	37.0	9
TATGCCG	45	2.570232E-9	37.0	13
TCTCGTA	45	2.570232E-9	37.0	8
TCGTATG	45	2.570232E-9	37.0	10
GATTATA	20	0.001833991	37.0	1
AAATCTC	50	7.192284E-9	33.3	5
GCCGTCT	50	7.192284E-9	33.3	16
TCTAGCG	30	3.5764585E-4	30.833334	28
CTAGCGG	30	3.5764585E-4	30.833334	29
AGAAATC	55	1.8204446E-8	30.272728	3
GTATGCC	55	1.8204446E-8	30.272728	12
ATGCCGT	55	1.8204446E-8	30.272728	14
CGGCGGA	25	0.005472908	29.599998	12
TGCTTGA	45	3.960822E-6	28.777779	25
TGAAAAA	45	3.960822E-6	28.777779	29
GCTTGAA	45	3.960822E-6	28.777779	26
GAAAAAA	45	3.960822E-6	28.777779	30
>>END_MODULE