FastQCFastQC Report
Fri 10 Feb 2017
ERR1631522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592153
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.21886235483059277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10250.17309715563376357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9340.15772950571896113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG358.864296E-426.42857210
GTACGTA358.864296E-426.42857213
GGTATCA5550.023.3333361
CCGTATC400.001930087223.1250029
TACGTAG400.001930087223.12500214
TGACTCG400.001930087223.12500210
GCAGTCG2200.021.8636369
TCGGTGA2150.021.51162713
CGGTGAT2400.020.81249814
CGACGTG450.003823649620.55555718
CCTCACG450.003823649620.55555728
CGGTGTA450.003823649620.55555734
TGTACGT450.003823649620.55555712
CCGCTTA555.1390484E-420.18181825
ATCCCGT555.1390484E-420.1818186
CAGTCGG2550.019.58823610
ATACACA1750.019.0285737
CTTATAC8550.018.60818737
ACGCTAT609.229608E-418.510
GTATACT701.21793084E-418.54
Produced by FastQC (version 0.11.5)