##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631522.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592153
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.520009186814896	34.0	31.0	34.0	31.0	34.0
2	32.725682382762564	34.0	31.0	34.0	31.0	34.0
3	32.842159036600336	34.0	31.0	34.0	31.0	34.0
4	36.285409345219904	37.0	37.0	37.0	35.0	37.0
5	36.18077760308569	37.0	35.0	37.0	35.0	37.0
6	36.211850653462875	37.0	37.0	37.0	35.0	37.0
7	36.22018802572984	37.0	36.0	37.0	35.0	37.0
8	36.20341195603164	37.0	36.0	37.0	35.0	37.0
9	38.00037659186055	39.0	38.0	39.0	35.0	39.0
10	37.90618809665745	39.0	38.0	39.0	35.0	39.0
11	37.975935273485064	39.0	38.0	39.0	35.0	39.0
12	37.75015916494554	39.0	38.0	39.0	35.0	39.0
13	37.90450778768325	39.0	38.0	39.0	35.0	39.0
14	39.31888211323763	40.0	39.0	41.0	36.0	41.0
15	39.29289558610697	40.0	39.0	41.0	36.0	41.0
16	39.26524901503497	40.0	39.0	41.0	36.0	41.0
17	39.24987461010921	40.0	39.0	41.0	36.0	41.0
18	39.2310078645215	40.0	39.0	41.0	36.0	41.0
19	39.2429524126366	40.0	39.0	41.0	36.0	41.0
20	39.199088749022636	40.0	39.0	41.0	36.0	41.0
21	39.18656158121296	40.0	39.0	41.0	36.0	41.0
22	39.140235716107156	40.0	39.0	41.0	36.0	41.0
23	39.07679603075557	40.0	39.0	41.0	36.0	41.0
24	39.052893424503466	40.0	39.0	41.0	36.0	41.0
25	39.050211685155695	40.0	39.0	41.0	36.0	41.0
26	38.940645407521366	40.0	38.0	41.0	35.0	41.0
27	38.884717294347915	40.0	38.0	41.0	35.0	41.0
28	38.82038594755072	40.0	38.0	41.0	35.0	41.0
29	38.74184881272239	40.0	38.0	41.0	35.0	41.0
30	38.69051072949052	40.0	38.0	41.0	35.0	41.0
31	38.62535696010997	40.0	38.0	41.0	35.0	41.0
32	38.558896096110296	40.0	38.0	41.0	35.0	41.0
33	38.46261017000674	40.0	38.0	41.0	35.0	41.0
34	38.4409654261652	40.0	38.0	41.0	34.0	41.0
35	38.34914456230062	40.0	38.0	41.0	34.0	41.0
36	38.30719763304416	40.0	38.0	41.0	34.0	41.0
37	38.250129611772635	40.0	38.0	41.0	34.0	41.0
38	38.17270705375131	40.0	38.0	41.0	34.0	41.0
39	38.09891024785824	40.0	38.0	41.0	34.0	41.0
40	38.02251107399608	40.0	37.0	41.0	34.0	41.0
41	37.93173892558173	40.0	37.0	41.0	33.0	41.0
42	37.82403871972277	40.0	37.0	41.0	33.0	41.0
43	36.891590517991126	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	13.0
18	11.0
19	25.0
20	62.0
21	123.0
22	191.0
23	310.0
24	488.0
25	723.0
26	1061.0
27	1488.0
28	2336.0
29	3262.0
30	4484.0
31	5777.0
32	8006.0
33	10902.0
34	15795.0
35	23671.0
36	38241.0
37	74950.0
38	175146.0
39	225084.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.182341388120975	18.795986847993678	12.674427048414852	26.347244715470495
2	19.271708494257396	19.693558928182412	34.222911983896054	26.811820593664137
3	19.56031633716286	22.39286130442639	28.544143152192085	29.502679206218662
4	14.879262623004527	15.877484366371528	34.41610529711071	34.82714771351323
5	15.693916943762845	35.25119352599751	33.30389274393611	15.750996786303538
6	33.97095007540281	35.001426996063515	15.869209477955867	15.158413450577807
7	30.466112643185124	29.229945639049365	21.26798310571761	19.0359586120479
8	27.75532674832349	32.52959961361338	19.43788176366581	20.277191874397328
9	27.13369686550604	14.570051996696801	19.40630208746726	38.889949050329896
10	18.840063294452616	25.227939400796757	31.39104251772768	24.540954787022947
11	36.98047632959725	21.720062213650866	20.60480990554806	20.694651551203826
12	22.83869202722945	25.52043137499937	27.30290988984266	24.337966707928523
13	30.874284179933227	18.801728607302504	24.08212066813813	26.241866544626134
14	23.60707452296957	19.894858254538946	24.135991880476837	32.36207534201465
15	26.470354790062707	26.614236523330963	21.143184278387512	25.772224408218825
16	26.228187647449225	24.841383899093646	23.28114524455673	25.649283208900403
17	24.73229047222593	25.785565554848155	24.250658191379593	25.231485781546326
18	24.95436145725851	24.187498838982492	25.364559497292085	25.49358020646691
19	25.554206429757176	24.588915364779034	25.411675698679225	24.445202506784565
20	25.569405204398187	24.25302244521264	24.962467470400387	25.215104879988786
21	26.30418152065429	24.591110743338294	24.4354077409048	24.669299995102616
22	26.747141363802935	23.813102356992196	24.239174672761937	25.200581606442928
23	25.394788171300327	24.115051346526993	24.85540054681814	25.634759935354545
24	24.74225411337948	24.654101220461605	24.382212029661254	26.22143263649766
25	25.693021904811765	23.663647739688898	25.049438236401738	25.5938921190976
26	25.17710794338625	24.696995540004018	24.993878271325148	25.13201824528458
27	25.320989676654516	24.355529736402584	24.4354077409048	25.888072846038103
28	24.998437903717452	24.330873946429385	25.720210823891797	24.950477325961366
29	25.00434853830007	24.3568807385929	25.694372907002077	24.94439781610496
30	24.64903496224793	24.8636754352338	25.14299513808087	25.3442944644374
31	25.47939468346863	24.35181448037923	24.685680896660152	25.48310993949199
32	24.476782182983115	23.895344615327456	25.45136138801965	26.176511813669777
33	24.118766602550355	23.837927022239185	25.894490106442085	26.14881626876838
34	25.54086528312784	22.932248928908578	25.787085432312256	25.739800355651326
35	24.89153985540899	23.540031039275323	26.320900172759405	25.247528932556285
36	24.304529403718295	23.679184264877488	25.80143983058433	26.21484650081989
37	24.487252449958035	22.17940295835705	26.72569420403173	26.60765038765319
38	23.485146575293886	22.460242538668215	27.761575133453686	26.293035752584217
39	23.32792369539629	22.196965986831106	28.382529515175975	26.092580802596625
40	23.285704876949033	22.12941587731549	28.998417638684597	25.586461607050882
41	22.240704682742468	22.137690765731154	29.58086845798299	26.040736093543394
42	22.108981969187017	22.723687965779117	29.420943573704765	25.746386491329098
43	21.44175576244653	21.98384539130934	29.740962217534992	26.833436628709133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	58.5
2	64.0
3	130.5
4	197.0
5	197.0
6	239.0
7	281.0
8	265.0
9	249.0
10	368.0
11	487.0
12	487.0
13	853.5
14	1220.0
15	1859.5
16	2499.0
17	2331.5
18	2164.0
19	2164.0
20	2384.5
21	2605.0
22	2330.5
23	2056.0
24	2388.0
25	2720.0
26	2720.0
27	3477.0
28	4234.0
29	5847.0
30	7460.0
31	9148.0
32	10836.0
33	10836.0
34	13908.0
35	16980.0
36	20460.5
37	23941.0
38	27228.0
39	30515.0
40	30515.0
41	33660.0
42	36805.0
43	39554.5
44	42304.0
45	44365.5
46	46427.0
47	46427.0
48	47975.5
49	49524.0
50	49146.0
51	48768.0
52	47784.0
53	46800.0
54	46800.0
55	44613.0
56	42426.0
57	38992.0
58	35558.0
59	34231.0
60	32904.0
61	32904.0
62	30300.0
63	27696.0
64	24799.5
65	21903.0
66	19557.0
67	17211.0
68	17211.0
69	14614.0
70	12017.0
71	10354.5
72	8692.0
73	6917.5
74	5143.0
75	5143.0
76	4200.0
77	3257.0
78	2725.0
79	2193.0
80	1901.5
81	1610.0
82	1610.0
83	1285.0
84	960.0
85	803.0
86	646.0
87	555.5
88	465.0
89	465.0
90	341.0
91	217.0
92	130.0
93	43.0
94	30.0
95	17.0
96	17.0
97	11.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	592153.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.333308115149784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.15104280153874	49.96657589497227
2	6.257685196599147	7.175475869284612
3	1.9549449318376013	3.362503803755871
4	0.9999196374322351	2.293148026531648
5	0.6121958264979178	1.7549605973706948
6	0.45516786217285177	1.5657767571642083
7	0.3405166913764228	1.3666063869525071
8	0.2433260993353867	1.1160552180522614
9	0.20344018910983477	1.049750914071465
>10	1.5399117969654417	17.36307718013578
>50	0.17798665160927085	6.974869920097558
>100	0.06297533892061428	5.4594410945210035
>500	2.9565886817189084E-4	0.15832328320807687
>1k	5.913177363437817E-4	0.3934350538821696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1296	0.21886235483059277	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1025	0.17309715563376357	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	934	0.15772950571896113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6887527378903764E-4
2	0.0	0.0	0.0	0.0	1.6887527378903764E-4
3	0.0	0.0	0.0	0.0	1.6887527378903764E-4
4	0.0	0.0	0.0	0.0	1.6887527378903764E-4
5	0.0	0.0	0.0	0.0	1.6887527378903764E-4
6	0.0	0.0	0.0	0.0	1.6887527378903764E-4
7	0.0	1.6887527378903764E-4	0.0	0.0	1.6887527378903764E-4
8	0.0	1.6887527378903764E-4	0.0	0.0	1.6887527378903764E-4
9	0.0	1.6887527378903764E-4	0.0	0.0	1.6887527378903764E-4
10	0.0	1.6887527378903764E-4	0.0	0.0	1.6887527378903764E-4
11	0.0	1.6887527378903764E-4	0.0	0.0	1.6887527378903764E-4
12	0.0	1.6887527378903764E-4	0.0	1.6887527378903764E-4	1.6887527378903764E-4
13	0.0	1.6887527378903764E-4	0.0	1.6887527378903764E-4	1.6887527378903764E-4
14	0.0	1.6887527378903764E-4	0.0	5.066258213671129E-4	1.6887527378903764E-4
15	0.0	1.6887527378903764E-4	0.0	8.443763689451882E-4	1.6887527378903764E-4
16	0.0	1.6887527378903764E-4	0.0	0.001351002190312301	3.377505475780753E-4
17	0.0	1.6887527378903764E-4	0.0	0.0016887527378903764	3.377505475780753E-4
18	0.0	1.6887527378903764E-4	0.0	0.001857628011679414	3.377505475780753E-4
19	0.0	1.6887527378903764E-4	0.0	0.0021953785592574893	5.066258213671129E-4
20	0.0	1.6887527378903764E-4	0.0	0.003208630201991715	5.066258213671129E-4
21	0.0	1.6887527378903764E-4	0.0	0.0043907571185149786	5.066258213671129E-4
22	0.0	1.6887527378903764E-4	0.0	0.00574175930882728	5.066258213671129E-4
23	0.0	1.6887527378903764E-4	0.0	0.007092761499139581	5.066258213671129E-4
24	0.0	1.6887527378903764E-4	0.0	0.011145768070076483	5.066258213671129E-4
25	0.0	1.6887527378903764E-4	0.0	0.013847772450701085	5.066258213671129E-4
26	0.0	1.6887527378903764E-4	0.0	0.01908290593816125	5.066258213671129E-4
27	0.0	1.6887527378903764E-4	0.0	0.053195711243546856	5.066258213671129E-4
28	0.0	1.6887527378903764E-4	0.0	0.19775294560696308	5.066258213671129E-4
29	0.0	1.6887527378903764E-4	0.0	0.39466151484498097	5.066258213671129E-4
30	0.0	1.6887527378903764E-4	0.0	0.6618221979792385	5.066258213671129E-4
31	0.0	1.6887527378903764E-4	0.0	1.463135372108222	5.066258213671129E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	35	8.864296E-4	26.428572	10
GTACGTA	35	8.864296E-4	26.428572	13
GGTATCA	555	0.0	23.333336	1
CCGTATC	40	0.0019300872	23.125002	9
TACGTAG	40	0.0019300872	23.125002	14
TGACTCG	40	0.0019300872	23.125002	10
GCAGTCG	220	0.0	21.863636	9
TCGGTGA	215	0.0	21.511627	13
CGGTGAT	240	0.0	20.812498	14
CGACGTG	45	0.0038236496	20.555557	18
CCTCACG	45	0.0038236496	20.555557	28
CGGTGTA	45	0.0038236496	20.555557	34
TGTACGT	45	0.0038236496	20.555557	12
CCGCTTA	55	5.1390484E-4	20.181818	25
ATCCCGT	55	5.1390484E-4	20.181818	6
CAGTCGG	255	0.0	19.588236	10
ATACACA	175	0.0	19.02857	37
CTTATAC	855	0.0	18.608187	37
ACGCTAT	60	9.229608E-4	18.5	10
GTATACT	70	1.21793084E-4	18.5	4
>>END_MODULE