FastQCFastQC Report
Fri 10 Feb 2017
ERR1631521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA211.4502762430939227TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60.4143646408839779No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAA60.4143646408839779TruSeq Adapter, Index 25 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.3453038674033149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40.2762430939226519No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA40.2762430939226519No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA30.20718232044198895No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCG30.20718232044198895No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAA30.20718232044198895TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC30.20718232044198895No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC20.13812154696132595No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG20.13812154696132595No Hit
TCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA20.13812154696132595No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG20.13812154696132595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13812154696132595No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAC20.13812154696132595TruSeq Adapter, Index 25 (96% over 27bp)
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT20.13812154696132595No Hit
CTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTG20.13812154696132595No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG20.13812154696132595No Hit
GAGTACATGGGATGCTAACTAGTTACGCGACCCCCGAGCGGTC20.13812154696132595No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG20.13812154696132595No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20.13812154696132595No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC20.13812154696132595No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.13812154696132595No Hit
TTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCA20.13812154696132595No Hit
CTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTT20.13812154696132595No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13812154696132595No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATA20.13812154696132595TruSeq Adapter, Index 25 (96% over 27bp)
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC20.13812154696132595No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCA20.13812154696132595No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAA20.13812154696132595No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG20.13812154696132595No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGC20.13812154696132595No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC20.13812154696132595No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG20.13812154696132595No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGAT20.13812154696132595No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACTAAA20.13812154696132595TruSeq Adapter, Index 25 (96% over 27bp)

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)