##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631521.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1448
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5414364640884	31.0	31.0	34.0	30.0	34.0
2	31.69889502762431	33.0	31.0	34.0	28.0	34.0
3	31.77693370165746	33.0	31.0	34.0	30.0	34.0
4	35.521408839779006	37.0	35.0	37.0	33.0	37.0
5	35.4896408839779	37.0	35.0	37.0	33.0	37.0
6	35.40953038674033	37.0	35.0	37.0	33.0	37.0
7	35.389502762430936	37.0	35.0	37.0	33.0	37.0
8	35.46339779005525	37.0	35.0	37.0	33.0	37.0
9	37.216850828729285	39.0	37.0	39.0	33.0	39.0
10	37.062845303867405	39.0	37.0	39.0	33.0	39.0
11	37.152624309392266	39.0	37.0	39.0	33.0	39.0
12	36.88812154696133	39.0	37.0	39.0	32.0	39.0
13	36.95718232044199	39.0	37.0	39.0	33.0	39.0
14	37.96339779005525	40.0	37.0	41.0	32.0	41.0
15	38.11602209944751	40.0	37.0	41.0	33.0	41.0
16	38.06353591160221	40.0	38.0	41.0	33.0	41.0
17	38.261740331491715	40.0	38.0	41.0	33.0	41.0
18	38.24378453038674	40.0	38.0	41.0	33.0	41.0
19	38.334944751381215	40.0	38.0	41.0	34.0	41.0
20	37.88812154696133	40.0	37.0	41.0	32.0	41.0
21	38.03176795580111	40.0	38.0	41.0	32.0	41.0
22	38.12154696132597	40.0	37.0	41.0	33.0	41.0
23	38.030386740331494	40.0	37.0	41.0	32.0	41.0
24	38.030386740331494	40.0	37.0	41.0	33.0	41.0
25	37.9171270718232	40.0	37.0	41.0	32.0	41.0
26	37.75759668508287	40.0	37.0	41.0	32.0	41.0
27	37.504834254143645	40.0	37.0	41.0	31.0	41.0
28	37.72790055248619	40.0	37.0	41.0	32.0	41.0
29	37.569060773480665	40.0	37.0	41.0	31.0	41.0
30	37.22375690607735	39.0	36.0	41.0	31.0	41.0
31	36.99033149171271	39.0	36.0	41.0	30.0	41.0
32	37.001381215469614	39.0	36.0	41.0	30.0	41.0
33	36.847375690607734	39.0	35.0	41.0	30.0	41.0
34	36.71477900552486	39.0	35.0	41.0	30.0	41.0
35	36.47444751381215	39.0	35.0	41.0	30.0	41.0
36	36.418508287292816	39.0	35.0	41.0	30.0	41.0
37	35.33425414364641	39.0	35.0	40.0	24.0	41.0
38	35.04903314917127	39.0	34.0	40.0	22.0	41.0
39	34.75621546961326	38.0	34.0	41.0	20.0	41.0
40	34.84046961325967	39.0	34.0	41.0	20.0	41.0
41	34.745165745856355	38.0	33.0	40.0	21.0	41.0
42	34.55524861878453	38.0	33.0	40.0	20.0	41.0
43	33.63812154696133	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	2.0
24	5.0
25	2.0
26	17.0
27	12.0
28	25.0
29	38.0
30	45.0
31	48.0
32	56.0
33	66.0
34	89.0
35	68.0
36	117.0
37	161.0
38	297.0
39	399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.37845303867403	15.814917127071823	11.533149171270718	23.273480662983424
2	19.544198895027623	18.370165745856355	32.18232044198895	29.903314917127073
3	18.853591160220994	29.696132596685082	26.65745856353591	24.79281767955801
4	20.85635359116022	14.77900552486188	31.077348066298345	33.28729281767956
5	15.05524861878453	41.43646408839779	29.074585635359117	14.433701657458563
6	31.629834254143645	39.571823204419886	15.26243093922652	13.535911602209943
7	27.140883977900554	37.36187845303867	17.886740331491712	17.61049723756906
8	26.726519337016576	27.624309392265197	25.06906077348066	20.58011049723757
9	26.035911602209943	12.430939226519337	18.99171270718232	42.5414364640884
10	18.784530386740332	22.92817679558011	35.98066298342542	22.306629834254142
11	32.04419889502763	22.513812154696133	19.26795580110497	26.174033149171272
12	31.077348066298345	23.549723756906076	23.895027624309392	21.477900552486187
13	29.696132596685082	16.988950276243095	30.801104972375693	22.513812154696133
14	21.201657458563535	28.107734806629836	21.54696132596685	29.14364640883978
15	23.825966850828728	25.345303867403313	27.279005524861876	23.549723756906076
16	31.284530386740332	24.102209944751383	20.511049723756905	24.102209944751383
17	22.72099447513812	23.825966850828728	21.96132596685083	31.491712707182316
18	21.754143646408842	23.273480662983424	23.895027624309392	31.077348066298345
19	31.560773480662984	23.756906077348066	21.616022099447513	23.066298342541437
20	23.756906077348066	21.408839779005525	32.73480662983425	22.099447513812155
21	24.79281767955801	23.549723756906076	23.1353591160221	28.52209944751381
22	24.171270718232044	23.273480662983424	28.107734806629836	24.447513812154696
23	24.24033149171271	21.96132596685083	31.767955801104975	22.030386740331494
24	25.828729281767952	20.994475138121548	22.513812154696133	30.662983425414364
25	23.825966850828728	20.234806629834253	31.215469613259668	24.723756906077348
26	31.629834254143645	21.892265193370168	22.997237569060776	23.480662983425415
27	22.168508287292816	23.1353591160221	22.790055248618785	31.906077348066297
28	22.099447513812155	21.616022099447513	31.629834254143645	24.654696132596683
29	21.96132596685083	22.513812154696133	32.73480662983425	22.790055248618785
30	31.767955801104975	21.408839779005525	24.378453038674035	22.444751381215468
31	23.756906077348066	30.11049723756906	23.066298342541437	23.066298342541437
32	22.997237569060776	30.17955801104972	22.168508287292816	24.654696132596683
33	22.85911602209945	31.97513812154696	22.582872928176794	22.582872928176794
34	22.030386740331494	30.0414364640884	24.516574585635357	23.41160220994475
35	23.6878453038674	30.455801104972373	23.61878453038674	22.23756906077348
36	22.85911602209945	31.906077348066297	19.9585635359116	25.276243093922652
37	24.378453038674035	27.209944751381215	24.171270718232044	24.24033149171271
38	21.96132596685083	26.243093922651934	25.552486187845304	26.243093922651934
39	22.030386740331494	24.79281767955801	26.65745856353591	26.519337016574585
40	25.0	26.035911602209943	25.20718232044199	23.756906077348066
41	21.33977900552486	25.345303867403313	27.417127071823206	25.89779005524862
42	22.92817679558011	26.65745856353591	26.312154696132595	24.102209944751383
43	22.306629834254142	25.345303867403313	27.624309392265197	24.723756906077348
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	5.0
14	9.0
15	9.5
16	10.0
17	9.0
18	8.0
19	8.0
20	8.5
21	9.0
22	7.5
23	6.0
24	7.5
25	9.0
26	9.0
27	11.0
28	13.0
29	13.5
30	14.0
31	20.0
32	26.0
33	26.0
34	45.5
35	65.0
36	76.5
37	88.0
38	91.5
39	95.0
40	95.0
41	94.0
42	93.0
43	92.5
44	92.0
45	89.5
46	87.0
47	87.0
48	97.0
49	107.0
50	99.5
51	92.0
52	94.0
53	96.0
54	96.0
55	94.5
56	93.0
57	87.5
58	82.0
59	83.0
60	84.0
61	84.0
62	80.5
63	77.0
64	69.0
65	61.0
66	52.0
67	43.0
68	43.0
69	37.5
70	32.0
71	25.0
72	18.0
73	17.5
74	17.0
75	17.0
76	12.0
77	7.0
78	6.0
79	5.0
80	4.0
81	3.0
82	3.0
83	2.5
84	2.0
85	2.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1448.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.82044198895028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.30517115804807	92.26519337016575
2	1.9664967225054626	3.7292817679558015
3	0.29133284777858703	0.8287292817679558
4	0.14566642388929352	0.5524861878453038
5	0.07283321194464676	0.3453038674033149
6	0.14566642388929352	0.8287292817679558
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.07283321194464676	1.4502762430939227
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	21	1.4502762430939227	TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.4143646408839779	No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAA	6	0.4143646408839779	TruSeq Adapter, Index 25 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.3453038674033149	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.2762430939226519	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	4	0.2762430939226519	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	3	0.20718232044198895	No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCG	3	0.20718232044198895	No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAA	3	0.20718232044198895	TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	3	0.20718232044198895	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2	0.13812154696132595	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	2	0.13812154696132595	No Hit
TCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2	0.13812154696132595	No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG	2	0.13812154696132595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.13812154696132595	No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAC	2	0.13812154696132595	TruSeq Adapter, Index 25 (96% over 27bp)
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2	0.13812154696132595	No Hit
CTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTG	2	0.13812154696132595	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	2	0.13812154696132595	No Hit
GAGTACATGGGATGCTAACTAGTTACGCGACCCCCGAGCGGTC	2	0.13812154696132595	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	2	0.13812154696132595	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2	0.13812154696132595	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	2	0.13812154696132595	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.13812154696132595	No Hit
TTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCA	2	0.13812154696132595	No Hit
CTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTT	2	0.13812154696132595	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.13812154696132595	No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATA	2	0.13812154696132595	TruSeq Adapter, Index 25 (96% over 27bp)
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2	0.13812154696132595	No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCA	2	0.13812154696132595	No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAA	2	0.13812154696132595	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	2	0.13812154696132595	No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGC	2	0.13812154696132595	No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC	2	0.13812154696132595	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	2	0.13812154696132595	No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGAT	2	0.13812154696132595	No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACTAAA	2	0.13812154696132595	TruSeq Adapter, Index 25 (96% over 27bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.06906077348066299	0.0
25	0.0	0.0	0.0	0.06906077348066299	0.0
26	0.0	0.0	0.0	0.06906077348066299	0.0
27	0.0	0.0	0.0	0.13812154696132597	0.0
28	0.0	0.0	0.0	0.13812154696132597	0.0
29	0.0	0.0	0.0	0.3453038674033149	0.0
30	0.0	0.0	0.0	0.6906077348066298	0.0
31	0.0	0.0	0.0	1.3812154696132597	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE