FastQCFastQC Report
Fri 10 Feb 2017
ERR1631520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535029
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37160.6945417911926269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26090.48763711873561993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25680.4799739827186938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.19550342130987292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG251.2327437E-437.010
CGTATGC303.5969296E-430.83333411
CTTACCG250.0054937529.69
CTCGTAT358.863171E-426.4285729
TACCGTC551.900195E-523.5454547
TATGCCG400.00192984523.12500213
CGTCGTA603.721495E-521.58333410
TCTCGTA450.003823174920.5555578
CGGTCCA909.454561E-820.55555710
ATGCCGT450.003823174920.55555714
GCCGTCT450.003823174920.55555716
ATTTCGG555.138156E-420.18181829
ATACCGT759.250598E-619.7333346
CCGGTCC951.6719241E-719.4736849
GGTATCA18350.019.4577661
TCTTGCG1002.8677823E-718.52
CGAAAGA500.00703008718.54
ACCGCCT701.2176516E-418.512
GACCGTT609.22802E-418.57
ATTGTAC609.22802E-418.53
Produced by FastQC (version 0.11.5)