##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631520.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535029
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81096538692295	34.0	33.0	34.0	31.0	34.0
2	32.98299344521512	34.0	33.0	34.0	31.0	34.0
3	33.08661773473961	34.0	33.0	34.0	31.0	34.0
4	36.42978978709565	37.0	37.0	37.0	35.0	37.0
5	36.426619865465234	37.0	37.0	37.0	35.0	37.0
6	36.49098460083472	37.0	37.0	37.0	35.0	37.0
7	36.475748043563996	37.0	37.0	37.0	35.0	37.0
8	36.46670928117915	37.0	37.0	37.0	35.0	37.0
9	38.31670059006147	39.0	39.0	39.0	37.0	39.0
10	38.27495518934488	39.0	39.0	39.0	37.0	39.0
11	38.338054572742784	39.0	39.0	39.0	37.0	39.0
12	38.12859676765185	39.0	39.0	39.0	37.0	39.0
13	38.25459928340333	39.0	39.0	39.0	37.0	39.0
14	39.74791459902173	41.0	40.0	41.0	38.0	41.0
15	39.74634459066705	41.0	40.0	41.0	38.0	41.0
16	39.72775681318209	41.0	40.0	41.0	38.0	41.0
17	39.73397703675875	41.0	40.0	41.0	38.0	41.0
18	39.76008964000082	41.0	40.0	41.0	38.0	41.0
19	39.79937349190418	41.0	40.0	41.0	38.0	41.0
20	39.78162118315082	41.0	40.0	41.0	38.0	41.0
21	39.76027093858464	41.0	40.0	41.0	38.0	41.0
22	39.70635984217678	41.0	40.0	41.0	37.0	41.0
23	39.6506768791972	41.0	40.0	41.0	37.0	41.0
24	39.63236946034701	41.0	40.0	41.0	37.0	41.0
25	39.606963360864555	41.0	40.0	41.0	37.0	41.0
26	39.53406824676793	41.0	39.0	41.0	37.0	41.0
27	39.453369817337006	41.0	39.0	41.0	36.0	41.0
28	39.3787869442591	41.0	39.0	41.0	36.0	41.0
29	39.284324774918744	41.0	39.0	41.0	36.0	41.0
30	39.200419042706095	41.0	39.0	41.0	35.0	41.0
31	39.098843240272956	40.0	39.0	41.0	35.0	41.0
32	39.001865319449976	40.0	39.0	41.0	35.0	41.0
33	38.904737874021784	40.0	39.0	41.0	35.0	41.0
34	38.84923247151089	40.0	38.0	41.0	35.0	41.0
35	38.75460395604724	40.0	38.0	41.0	35.0	41.0
36	38.691867169816966	40.0	38.0	41.0	35.0	41.0
37	38.61387513574031	40.0	38.0	41.0	35.0	41.0
38	38.51274416900766	40.0	38.0	41.0	35.0	41.0
39	38.41820163019201	40.0	38.0	41.0	35.0	41.0
40	38.301615426453516	40.0	38.0	41.0	35.0	41.0
41	38.18271532944943	40.0	38.0	41.0	35.0	41.0
42	38.05389988206247	40.0	38.0	41.0	34.0	41.0
43	37.1870982694396	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	2.0
14	2.0
15	2.0
16	4.0
17	5.0
18	8.0
19	22.0
20	33.0
21	81.0
22	109.0
23	199.0
24	324.0
25	504.0
26	792.0
27	1228.0
28	1773.0
29	2556.0
30	3351.0
31	4168.0
32	5474.0
33	7251.0
34	10624.0
35	15343.0
36	24493.0
37	52175.0
38	110492.0
39	294009.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.21939371510703	19.057097839556363	14.565565604855063	24.157942840481546
2	18.391152629109826	21.345571922269635	35.74198781748279	24.52128763113775
3	18.670763640849376	23.551059849092294	30.917389524679972	26.860786985378365
4	13.393479605778378	17.424289150681552	35.88123260608304	33.300998637457035
5	14.088769019997047	36.79464103814934	33.73032116016142	15.386268781692209
6	30.32938401469827	36.776884991280845	17.477931102800035	15.41579989122085
7	27.88110551016861	31.807621642938983	21.380336392980567	18.930936453911844
8	27.39515054324158	32.9623254066602	20.33029985290517	19.312224197193046
9	26.953492240607517	14.721631911541245	20.40954789366558	37.91532795418566
10	18.11527973250048	26.664349035285937	31.278491446258055	23.94187978595553
11	34.004138093449136	23.46470938958449	21.061848983886854	21.469303533079515
12	22.036001786819032	28.182210683906856	27.831762390449867	21.95002513882425
13	30.04566107631549	20.0736782492164	25.721783305204017	24.158877369264093
14	22.653538406329375	21.74143831455865	26.2213823923563	29.383640886755668
15	25.763650194662347	27.386739784198618	23.224722398225143	23.6248876229139
16	23.65610088425113	26.82639632618045	25.172654192576477	24.344848596991937
17	21.644060415416735	28.00577164976104	26.125312833509962	24.224855101312265
18	22.33804148933983	25.43301391139546	28.203144128636016	24.025800470628695
19	23.463214143532408	26.744718510585408	28.007079990056617	21.78498735582557
20	24.706511235839553	25.10592883750227	28.029882492350882	22.1576774343073
21	26.00251575148263	25.35526111668713	26.498189817748198	22.14403331408204
22	25.326103818671513	25.12966586857909	26.318386479985197	23.225843832764205
23	23.619093544462075	25.602350526793877	26.832564216145293	23.945991712598758
24	22.70699345269135	26.397447614989094	27.320201334880913	23.575357597438643
25	23.08173949449469	25.424416246595978	27.38505763239002	24.10878662651931
26	23.405273359014185	25.77430382278344	27.25646647191087	23.56395634629151
27	23.366957678929552	25.767201404036044	26.65089182081719	24.214949096217214
28	22.58812139155074	25.79355511570401	28.41154404714511	23.206779445600144
29	22.57503798859501	26.07783877135632	28.603496259081286	22.743626980967388
30	22.71596492900385	26.13017238317923	28.244824112337835	22.909038575479084
31	22.91034691577466	25.97298464195399	27.463184238611365	23.65348420365999
32	21.69788927329173	25.567772961839452	28.827596261137245	23.906741503731574
33	21.978808625326852	24.802206983172876	29.530735717129353	23.68824867437092
34	23.10061697590224	24.142616568447693	28.87581794631693	23.880948509333138
35	22.3806560018242	24.333821157357825	28.989643552031758	24.295879288786214
36	21.162030469376425	24.22298604374716	29.29317850060464	25.321804986271772
37	21.256417876414176	22.87838603141138	30.384708118625348	25.4804879735491
38	20.67626240820591	22.170200119993495	31.71753306830097	25.436004403499624
39	20.543559321083528	22.141790445003913	32.80962340359121	24.50502683032135
40	20.02302678920208	22.571299873464802	32.80121264454824	24.604460692784876
41	18.653194499737396	22.836892953466073	33.83517528956374	24.674737257232785
42	18.46236372234028	23.42975801311705	33.13240964508466	24.97546861945801
43	17.432326098211497	23.212012806782436	33.22455418304428	26.131106911961783
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	49.5
2	71.0
3	287.0
4	503.0
5	503.0
6	645.5
7	788.0
8	806.5
9	825.0
10	1220.5
11	1616.0
12	1616.0
13	2659.5
14	3703.0
15	5712.5
16	7722.0
17	6864.0
18	6006.0
19	6006.0
20	6641.0
21	7276.0
22	6274.5
23	5273.0
24	5823.0
25	6373.0
26	6373.0
27	7169.5
28	7966.0
29	9591.5
30	11217.0
31	13112.0
32	15007.0
33	15007.0
34	17740.0
35	20473.0
36	23206.0
37	25939.0
38	28042.5
39	30146.0
40	30146.0
41	32203.5
42	34261.0
43	36460.5
44	38660.0
45	40099.5
46	41539.0
47	41539.0
48	42691.0
49	43843.0
50	42438.5
51	41034.0
52	39044.5
53	37055.0
54	37055.0
55	34214.0
56	31373.0
57	29036.5
58	26700.0
59	25155.0
60	23610.0
61	23610.0
62	21167.5
63	18725.0
64	16527.0
65	14329.0
66	12452.0
67	10575.0
68	10575.0
69	9112.5
70	7650.0
71	6643.0
72	5636.0
73	4436.5
74	3237.0
75	3237.0
76	2487.0
77	1737.0
78	1513.0
79	1289.0
80	1169.0
81	1049.0
82	1049.0
83	892.5
84	736.0
85	584.5
86	433.0
87	369.0
88	305.0
89	305.0
90	261.5
91	218.0
92	139.0
93	60.0
94	44.0
95	28.0
96	28.0
97	20.0
98	12.0
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	535029.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.57682144121084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.02773026877944	57.236769320556824
2	4.57840494799745	5.821608677287801
3	1.5417387810663006	2.9405655357852667
4	0.8273292996882656	2.103958686374515
5	0.5469501811831103	1.7386677003158257
6	0.3958372021523885	1.509964267261853
7	0.2915115621591415	1.2973364974808121
8	0.2237477652388974	1.1380137374770674
9	0.17278398343275259	0.9886550816344718
>10	1.2539505433851172	15.238584426834755
>50	0.10199021346703853	4.426881274154391
>100	0.036551404494618	3.602111686328118
>500	2.9476939108562754E-4	0.09426471974522853
>1k	0.0011790775643425102	1.862618388763074
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3716	0.6945417911926269	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2609	0.48763711873561993	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2568	0.4799739827186938	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1046	0.19550342130987292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8690575651039476E-4	0.0
2	0.0	1.8690575651039476E-4	0.0	1.8690575651039476E-4	0.0
3	0.0	1.8690575651039476E-4	0.0	1.8690575651039476E-4	0.0
4	0.0	1.8690575651039476E-4	0.0	1.8690575651039476E-4	0.0
5	0.0	1.8690575651039476E-4	0.0	1.8690575651039476E-4	0.0
6	0.0	1.8690575651039476E-4	0.0	1.8690575651039476E-4	0.0
7	0.0	1.8690575651039476E-4	0.0	1.8690575651039476E-4	0.0
8	0.0	1.8690575651039476E-4	0.0	1.8690575651039476E-4	0.0
9	0.0	1.8690575651039476E-4	0.0	5.607172695311843E-4	0.0
10	0.0	1.8690575651039476E-4	0.0	9.345287825519739E-4	0.0
11	0.0	1.8690575651039476E-4	0.0	0.001495246052083158	0.0
12	0.0	1.8690575651039476E-4	0.0	0.0018690575651039477	0.0
13	0.0	1.8690575651039476E-4	0.0	0.0020559633216143424	0.0
14	0.0	1.8690575651039476E-4	0.0	0.0022428690781247373	0.0
15	0.0	1.8690575651039476E-4	0.0	0.0033643036171871056	0.0
16	0.0	1.8690575651039476E-4	0.0	0.004859549669270264	1.8690575651039476E-4
17	0.0	1.8690575651039476E-4	0.0	0.006541701477863817	1.8690575651039476E-4
18	0.0	1.8690575651039476E-4	0.0	0.007102418747395001	1.8690575651039476E-4
19	0.0	1.8690575651039476E-4	0.0	0.008410759042967765	1.8690575651039476E-4
20	0.0	1.8690575651039476E-4	0.0	0.010279816608071712	1.8690575651039476E-4
21	0.0	1.8690575651039476E-4	0.0	0.012335779929686054	3.738115130207895E-4
22	0.0	1.8690575651039476E-4	0.0	0.015139366277341975	3.738115130207895E-4
23	0.0	1.8690575651039476E-4	0.0	0.024671559859372107	3.738115130207895E-4
24	0.0	1.8690575651039476E-4	0.0	0.03981092613671409	3.738115130207895E-4
25	0.0	1.8690575651039476E-4	0.0	0.04672643912759869	3.738115130207895E-4
26	0.0	1.8690575651039476E-4	0.0	0.06298723994400303	3.738115130207895E-4
27	0.0	1.8690575651039476E-4	0.0	0.14447814978253515	3.738115130207895E-4
28	0.0	3.738115130207895E-4	0.0	0.47586205607546506	5.607172695311843E-4
29	0.0	3.738115130207895E-4	0.0	0.9739658971756671	5.607172695311843E-4
30	0.0	3.738115130207895E-4	0.0	1.7518676557719302	5.607172695311843E-4
31	0.0	3.738115130207895E-4	0.0	3.4175717577925684	5.607172695311843E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	25	1.2327437E-4	37.0	10
CGTATGC	30	3.5969296E-4	30.833334	11
CTTACCG	25	0.00549375	29.6	9
CTCGTAT	35	8.863171E-4	26.428572	9
TACCGTC	55	1.900195E-5	23.545454	7
TATGCCG	40	0.001929845	23.125002	13
CGTCGTA	60	3.721495E-5	21.583334	10
TCTCGTA	45	0.0038231749	20.555557	8
CGGTCCA	90	9.454561E-8	20.555557	10
ATGCCGT	45	0.0038231749	20.555557	14
GCCGTCT	45	0.0038231749	20.555557	16
ATTTCGG	55	5.138156E-4	20.181818	29
ATACCGT	75	9.250598E-6	19.733334	6
CCGGTCC	95	1.6719241E-7	19.473684	9
GGTATCA	1835	0.0	19.457766	1
TCTTGCG	100	2.8677823E-7	18.5	2
CGAAAGA	50	0.007030087	18.5	4
ACCGCCT	70	1.2176516E-4	18.5	12
GACCGTT	60	9.22802E-4	18.5	7
ATTGTAC	60	9.22802E-4	18.5	3
>>END_MODULE