FastQCFastQC Report
Fri 10 Feb 2017
ERR1631519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100.5330490405117271No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA100.5330490405117271No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA80.42643923240938164TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50.26652452025586354No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT50.26652452025586354RNA PCR Primer, Index 39 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50.26652452025586354No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA50.26652452025586354No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT50.26652452025586354No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT50.26652452025586354No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC50.26652452025586354No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT40.21321961620469082No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA40.21321961620469082No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA40.21321961620469082No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC40.21321961620469082No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA40.21321961620469082No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA40.21321961620469082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.21321961620469082No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA40.21321961620469082No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA30.15991471215351813No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT30.15991471215351813No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA30.15991471215351813No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC30.15991471215351813ABI Solid3 Adapter B (100% over 23bp)
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA30.15991471215351813No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGC30.15991471215351813No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT30.15991471215351813No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG30.15991471215351813No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT30.15991471215351813No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT30.15991471215351813No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT30.15991471215351813No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT30.15991471215351813No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC30.15991471215351813No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT30.15991471215351813No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA20.10660980810234541No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC20.10660980810234541No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCT20.10660980810234541No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT20.10660980810234541No Hit
GGACGGTCTGAACACGCAGTTCAGCAACGCCGTCGCCGACGCG20.10660980810234541No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAG20.10660980810234541No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC20.10660980810234541No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC20.10660980810234541RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC20.10660980810234541RNA PCR Primer, Index 39 (95% over 21bp)
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG20.10660980810234541No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC20.10660980810234541No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAAC20.10660980810234541No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT20.10660980810234541No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT20.10660980810234541No Hit
TCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTCT20.10660980810234541No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA20.10660980810234541ABI Solid3 Adapter B (100% over 21bp)
CTCAAGCGCCTTAGAATACTCATCTCGCGCACCAGTGTCGGTT20.10660980810234541No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT20.10660980810234541No Hit
TATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATG20.10660980810234541No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT20.10660980810234541No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCT20.10660980810234541No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGA20.10660980810234541ABI Solid3 Adapter B (100% over 21bp)
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC20.10660980810234541No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGTATCAACTGTCT20.10660980810234541No Hit
TCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCAT20.10660980810234541No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT20.10660980810234541No Hit
TTAGAATACTCATCTCGCGCACCAGTGTCGGTTTGCGGTACGG20.10660980810234541No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20.10660980810234541No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT20.10660980810234541No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC20.10660980810234541No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATACTGTCT20.10660980810234541No Hit
CGAAGGCACGCAACGCCTGAAGGCCCGCGTCGTGGCGGAGCTG20.10660980810234541No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGA20.10660980810234541No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC20.10660980810234541No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT20.10660980810234541No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAACTGTCTC20.10660980810234541No Hit
TTCCTCGCCTTATTGATATGCCATAGAGAGCCCATGTACTCTG20.10660980810234541No Hit
GGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGC20.10660980810234541No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT20.10660980810234541No Hit
CGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCTCT20.10660980810234541No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)