##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631519.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1876
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3363539445629	34.0	31.0	34.0	30.0	34.0
2	32.43816631130064	34.0	31.0	34.0	31.0	34.0
3	32.554904051172706	34.0	31.0	34.0	30.0	34.0
4	35.99946695095949	37.0	35.0	37.0	35.0	37.0
5	35.96215351812367	37.0	35.0	37.0	35.0	37.0
6	36.088486140724946	37.0	35.0	37.0	35.0	37.0
7	36.03144989339019	37.0	35.0	37.0	35.0	37.0
8	36.05223880597015	37.0	35.0	37.0	35.0	37.0
9	37.877931769722814	39.0	38.0	39.0	35.0	39.0
10	37.840085287846485	39.0	38.0	39.0	35.0	39.0
11	37.88539445628998	39.0	38.0	39.0	35.0	39.0
12	37.69776119402985	39.0	38.0	39.0	35.0	39.0
13	37.832089552238806	39.0	38.0	39.0	35.0	39.0
14	39.09275053304904	40.0	38.0	41.0	36.0	41.0
15	39.06236673773987	40.0	38.0	41.0	36.0	41.0
16	38.96321961620469	40.0	38.0	41.0	36.0	41.0
17	39.06396588486141	40.0	38.0	41.0	36.0	41.0
18	39.05863539445629	40.0	39.0	41.0	36.0	41.0
19	39.11727078891258	40.0	39.0	41.0	36.0	41.0
20	39.088486140724946	40.0	39.0	41.0	36.0	41.0
21	39.12420042643923	40.0	39.0	41.0	36.0	41.0
22	39.10554371002132	40.0	39.0	41.0	35.0	41.0
23	38.85660980810235	40.0	38.0	41.0	35.0	41.0
24	38.87046908315565	40.0	39.0	41.0	35.0	41.0
25	38.87633262260128	40.0	38.0	41.0	35.0	41.0
26	38.841684434968016	40.0	38.0	41.0	35.0	41.0
27	38.6044776119403	40.0	38.0	41.0	35.0	41.0
28	38.55756929637526	40.0	38.0	41.0	34.0	41.0
29	38.41257995735608	40.0	38.0	41.0	34.0	41.0
30	38.40618336886994	40.0	38.0	41.0	34.0	41.0
31	38.15778251599147	40.0	38.0	41.0	33.0	41.0
32	38.134328358208954	40.0	38.0	41.0	33.0	41.0
33	38.02452025586354	40.0	38.0	41.0	33.0	41.0
34	38.051705756929636	40.0	38.0	41.0	33.0	41.0
35	37.88006396588486	40.0	37.0	41.0	33.0	41.0
36	37.80863539445629	40.0	37.0	41.0	33.0	41.0
37	37.47494669509595	40.0	37.0	41.0	33.0	41.0
38	37.30597014925373	40.0	37.0	41.0	31.0	41.0
39	37.2590618336887	40.0	36.0	41.0	32.0	41.0
40	37.05277185501066	40.0	36.0	41.0	31.0	41.0
41	37.00053304904051	40.0	36.0	41.0	31.0	41.0
42	36.887526652452024	40.0	35.0	41.0	31.0	41.0
43	35.96162046908316	38.0	35.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	4.0
25	5.0
26	11.0
27	9.0
28	13.0
29	27.0
30	22.0
31	29.0
32	36.0
33	54.0
34	49.0
35	102.0
36	119.0
37	202.0
38	373.0
39	820.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.29637526652452	15.671641791044777	13.699360341151387	26.332622601279315
2	18.550106609808104	18.176972281449892	33.95522388059701	29.31769722814499
3	19.189765458422176	23.134328358208954	29.26439232409382	28.411513859275058
4	17.32409381663113	16.57782515991471	31.769722814498934	34.32835820895522
5	15.778251599147122	38.272921108742004	29.21108742004264	16.73773987206823
6	32.782515991471215	31.343283582089555	18.017057569296373	17.857142857142858
7	32.569296375266525	30.11727078891258	17.91044776119403	19.402985074626866
8	30.11727078891258	28.94456289978678	21.162046908315567	19.776119402985074
9	28.94456289978678	12.526652452025585	16.89765458422175	41.631130063965884
10	20.255863539445627	23.02771855010661	28.624733475479747	28.091684434968016
11	33.74200426439232	22.921108742004265	17.537313432835823	25.79957356076759
12	26.01279317697228	27.025586353944565	23.8272921108742	23.134328358208954
13	33.368869936034116	16.95095948827292	26.972281449893394	22.707889125799575
14	24.040511727078894	23.454157782515992	22.441364605543708	30.06396588486141
15	29.957356076759062	22.228144989339018	21.535181236673772	26.279317697228144
16	28.411513859275058	23.560767590618337	23.61407249466951	24.4136460554371
17	21.80170575692964	27.505330490405118	21.162046908315567	29.53091684434968
18	24.093816631130064	21.748400852878465	24.733475479744136	29.42430703624733
19	26.01279317697228	27.665245202558637	24.466950959488273	21.85501066098081
20	26.652452025586353	21.108742004264393	30.2771855010661	21.961620469083158
21	29.637526652452024	19.936034115138593	24.520255863539443	25.906183368869932
22	28.464818763326228	19.56289978678038	24.520255863539443	27.452025586353944
23	27.611940298507463	19.456289978678036	24.573560767590617	28.35820895522388
24	25.15991471215352	22.547974413646056	24.520255863539443	27.77185501066098
25	24.78678038379531	22.228144989339018	25.85287846481876	27.132196162046906
26	31.236673773987206	20.362473347547976	22.388059701492537	26.01279317697228
27	26.119402985074625	21.85501066098081	22.494669509594882	29.53091684434968
28	22.388059701492537	20.84221748400853	28.30490405117271	28.464818763326228
29	22.281449893390192	21.908315565031984	29.637526652452024	26.172707889125803
30	23.560767590618337	23.187633262260128	26.918976545842217	26.332622601279315
31	23.88059701492537	26.01279317697228	22.334754797441363	27.77185501066098
32	23.240938166311302	25.15991471215352	27.345415778251596	24.253731343283583
33	23.98720682302772	23.560767590618337	29.05117270788913	23.40085287846482
34	25.95948827292111	23.933901918976545	22.707889125799575	27.398720682302773
35	23.98720682302772	24.14712153518124	26.385927505330493	25.479744136460553
36	21.375266524520256	25.639658848614072	26.332622601279315	26.652452025586353
37	24.680170575692966	22.921108742004265	26.652452025586353	25.74626865671642
38	22.761194029850746	20.62899786780384	28.14498933901919	28.464818763326228
39	24.680170575692966	20.46908315565032	29.637526652452024	25.21321961620469
40	24.4136460554371	19.616204690831555	27.985074626865668	27.985074626865668
41	21.162046908315567	22.6545842217484	29.744136460554373	26.439232409381663
42	22.707889125799575	20.3091684434968	29.850746268656714	27.132196162046906
43	18.976545842217483	22.921108742004265	32.142857142857146	25.95948827292111
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.0
6	1.5
7	1.0
8	1.5
9	2.0
10	1.5
11	1.0
12	1.0
13	5.0
14	9.0
15	12.0
16	15.0
17	13.0
18	11.0
19	11.0
20	10.0
21	9.0
22	8.0
23	7.0
24	9.0
25	11.0
26	11.0
27	14.5
28	18.0
29	16.5
30	15.0
31	15.5
32	16.0
33	16.0
34	29.0
35	42.0
36	43.5
37	45.0
38	56.5
39	68.0
40	68.0
41	67.0
42	66.0
43	84.0
44	102.0
45	121.0
46	140.0
47	140.0
48	181.0
49	222.0
50	200.5
51	179.0
52	178.5
53	178.0
54	178.0
55	145.0
56	112.0
57	107.5
58	103.0
59	106.0
60	109.0
61	109.0
62	105.0
63	101.0
64	93.0
65	85.0
66	78.0
67	71.0
68	71.0
69	59.5
70	48.0
71	40.0
72	32.0
73	27.5
74	23.0
75	23.0
76	20.0
77	17.0
78	11.5
79	6.0
80	5.5
81	5.0
82	5.0
83	4.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1876.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.27078891257996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.84055459272098	88.43283582089553
2	2.310803004043905	4.264392324093817
3	0.8087810514153668	2.2388059701492535
4	0.462160600808781	1.7057569296375266
5	0.4043905257076834	1.8656716417910446
6	0.0	0.0
7	0.0	0.0
8	0.05777007510109763	0.42643923240938164
9	0.0	0.0
>10	0.11554015020219525	1.0660980810234542
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.5330490405117271	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	10	0.5330490405117271	No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	8	0.42643923240938164	TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.26652452025586354	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	5	0.26652452025586354	RNA PCR Primer, Index 39 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.26652452025586354	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	5	0.26652452025586354	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	5	0.26652452025586354	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	5	0.26652452025586354	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	5	0.26652452025586354	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	4	0.21321961620469082	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	4	0.21321961620469082	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	4	0.21321961620469082	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	4	0.21321961620469082	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	4	0.21321961620469082	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	4	0.21321961620469082	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.21321961620469082	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	4	0.21321961620469082	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	3	0.15991471215351813	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACCTGTCT	3	0.15991471215351813	No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA	3	0.15991471215351813	No Hit
CCTCTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGC	3	0.15991471215351813	ABI Solid3 Adapter B (100% over 23bp)
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA	3	0.15991471215351813	No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGC	3	0.15991471215351813	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	3	0.15991471215351813	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	3	0.15991471215351813	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	3	0.15991471215351813	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	3	0.15991471215351813	No Hit
CAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCTT	3	0.15991471215351813	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	3	0.15991471215351813	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	3	0.15991471215351813	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	3	0.15991471215351813	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	2	0.10660980810234541	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2	0.10660980810234541	No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCT	2	0.10660980810234541	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	2	0.10660980810234541	No Hit
GGACGGTCTGAACACGCAGTTCAGCAACGCCGTCGCCGACGCG	2	0.10660980810234541	No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAG	2	0.10660980810234541	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	2	0.10660980810234541	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	2	0.10660980810234541	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	2	0.10660980810234541	RNA PCR Primer, Index 39 (95% over 21bp)
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG	2	0.10660980810234541	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2	0.10660980810234541	No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAAC	2	0.10660980810234541	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	2	0.10660980810234541	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	2	0.10660980810234541	No Hit
TCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTCT	2	0.10660980810234541	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	2	0.10660980810234541	ABI Solid3 Adapter B (100% over 21bp)
CTCAAGCGCCTTAGAATACTCATCTCGCGCACCAGTGTCGGTT	2	0.10660980810234541	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	2	0.10660980810234541	No Hit
TATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATG	2	0.10660980810234541	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	2	0.10660980810234541	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCT	2	0.10660980810234541	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGA	2	0.10660980810234541	ABI Solid3 Adapter B (100% over 21bp)
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	2	0.10660980810234541	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGTATCAACTGTCT	2	0.10660980810234541	No Hit
TCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCAT	2	0.10660980810234541	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	2	0.10660980810234541	No Hit
TTAGAATACTCATCTCGCGCACCAGTGTCGGTTTGCGGTACGG	2	0.10660980810234541	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2	0.10660980810234541	No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT	2	0.10660980810234541	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC	2	0.10660980810234541	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATACTGTCT	2	0.10660980810234541	No Hit
CGAAGGCACGCAACGCCTGAAGGCCCGCGTCGTGGCGGAGCTG	2	0.10660980810234541	No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGA	2	0.10660980810234541	No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTC	2	0.10660980810234541	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTAT	2	0.10660980810234541	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAACTGTCTC	2	0.10660980810234541	No Hit
TTCCTCGCCTTATTGATATGCCATAGAGAGCCCATGTACTCTG	2	0.10660980810234541	No Hit
GGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGC	2	0.10660980810234541	No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	2	0.10660980810234541	No Hit
CGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCTCT	2	0.10660980810234541	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.10660980810234541	0.0
29	0.0	0.0	0.0	0.21321961620469082	0.0
30	0.0	0.0	0.0	0.47974413646055436	0.0
31	0.0	0.0	0.0	1.2260127931769722	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE