FastQCFastQC Report
Fri 10 Feb 2017
ERR1631518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318384
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.3524046434494196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8690.27294085129906026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7510.2358786873712247No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC3970.12469219558771798No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3770.11841047288808483No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3530.11087240564852505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCC250.0054904529.59999831
GTGTAAA400.001928137823.1251
ATGGTCG400.001928137823.12536
ATACACA1054.0017767E-1122.9047637
TCTAGCG502.69616E-422.19999928
CTAGCGG502.69616E-422.19999929
GGTATCA4150.020.9518071
TCGTTTA450.003819824220.55555730
TCAGGCG450.003819824220.5555574
CGTTTAT450.003819824220.55555731
CTTATAC4200.020.26190437
AGACAGT555.131875E-420.1818186
TATACAC1202.3646862E-1020.04166636
GACAGTG609.216834E-418.57
GTCCTAA609.216834E-418.51
GCAGTCG2200.018.59
GCCTGTA609.216834E-418.52
AGATAAC500.007023987418.4999985
ATCGTTT500.007023987418.49999829
TATACAA500.007023987418.4999985
Produced by FastQC (version 0.11.5)