##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631518.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	318384
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.447195838986886	34.0	31.0	34.0	31.0	34.0
2	32.651147670737224	34.0	31.0	34.0	31.0	34.0
3	32.7576794060003	34.0	31.0	34.0	31.0	34.0
4	36.24440926679733	37.0	37.0	37.0	35.0	37.0
5	36.10681127192321	37.0	35.0	37.0	35.0	37.0
6	36.13801258857229	37.0	36.0	37.0	35.0	37.0
7	36.13933175033921	37.0	36.0	37.0	35.0	37.0
8	36.12094514799739	37.0	36.0	37.0	35.0	37.0
9	37.904216292276	39.0	38.0	39.0	35.0	39.0
10	37.804245816372685	39.0	38.0	39.0	35.0	39.0
11	37.884029976380724	39.0	38.0	39.0	35.0	39.0
12	37.66282225237449	39.0	37.0	39.0	35.0	39.0
13	37.799349213528316	39.0	38.0	39.0	35.0	39.0
14	39.1789380119604	40.0	39.0	41.0	36.0	41.0
15	39.14929142167948	40.0	39.0	41.0	36.0	41.0
16	39.11147231016634	40.0	38.0	41.0	36.0	41.0
17	39.10511520679431	40.0	38.0	41.0	36.0	41.0
18	39.08764259510528	40.0	38.0	41.0	36.0	41.0
19	39.10148437107392	40.0	39.0	41.0	36.0	41.0
20	39.06866236996834	40.0	39.0	41.0	36.0	41.0
21	39.03289738177798	40.0	39.0	41.0	36.0	41.0
22	38.9981971455852	40.0	38.0	41.0	36.0	41.0
23	38.93477373234836	40.0	38.0	41.0	35.0	41.0
24	38.89520830192472	40.0	38.0	41.0	35.0	41.0
25	38.87673061460375	40.0	38.0	41.0	35.0	41.0
26	38.78215299763807	40.0	38.0	41.0	35.0	41.0
27	38.70789675360571	40.0	38.0	41.0	35.0	41.0
28	38.628200537715465	40.0	38.0	41.0	35.0	41.0
29	38.534894969596465	40.0	38.0	41.0	35.0	41.0
30	38.472404392180515	40.0	38.0	41.0	35.0	41.0
31	38.392915473139354	40.0	38.0	41.0	34.0	41.0
32	38.29859854766571	40.0	38.0	41.0	34.0	41.0
33	38.223076536509375	40.0	38.0	41.0	34.0	41.0
34	38.16961593547414	40.0	38.0	41.0	34.0	41.0
35	38.057487185285694	40.0	38.0	41.0	34.0	41.0
36	38.005694381627215	40.0	37.0	41.0	33.0	41.0
37	37.93971430725162	40.0	37.0	41.0	33.0	41.0
38	37.833499798984874	40.0	37.0	41.0	33.0	41.0
39	37.74938439117543	40.0	37.0	41.0	33.0	41.0
40	37.66006457610935	40.0	37.0	41.0	33.0	41.0
41	37.54233252927283	40.0	37.0	41.0	33.0	41.0
42	37.42352944871602	40.0	36.0	41.0	33.0	41.0
43	36.44301221166893	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	3.0
18	18.0
19	14.0
20	44.0
21	87.0
22	126.0
23	221.0
24	326.0
25	457.0
26	694.0
27	1006.0
28	1484.0
29	1963.0
30	2798.0
31	3741.0
32	4838.0
33	6607.0
34	9518.0
35	14328.0
36	23427.0
37	44669.0
38	95293.0
39	106720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.445286195286194	18.269448213478064	13.15549776370672	26.129767827529022
2	19.00566611387507	20.23751193527313	34.40154027840595	26.35528167244585
3	20.204846977235036	21.601273933363487	29.68051158349666	28.513367505904817
4	15.26490024624353	15.932333283079553	33.0462586059601	35.756507864716816
5	15.781257852153374	35.498014975626916	32.646741042263436	16.073986129956282
6	34.4298080305543	33.86916427961204	16.062993115231922	15.63803457460174
7	30.297690838735612	30.170800040203027	19.965513342379012	19.565995778682346
8	28.349414543444396	32.615960601035226	19.30561837278255	19.729006482737827
9	27.39365043469521	14.725614352480024	18.97174481129705	38.90899040152772
10	18.560606060606062	26.044650484948995	30.638474295190715	24.756269159254234
11	36.70504799236142	21.565154027840595	20.41999597969747	21.309802000100508
12	22.958754208754208	25.325393235841	27.023342881551837	24.692509673852957
13	30.849540177898387	18.415184180109552	24.88504447459671	25.85023116739535
14	23.52882054374592	20.47684557012915	23.57310668877833	32.4212271973466
15	26.864101211116136	26.72715965626413	21.20364088647671	25.20509824614302
16	26.181906125935978	25.491230715111314	22.92137795869139	25.405485200261317
17	24.976129453741393	25.89797225991256	24.0034046937032	25.122493592642847
18	24.620269862807177	23.88970551283984	25.802490074878136	25.68753454947485
19	25.628486356098296	25.117782300618117	25.470501030202524	23.78323031308106
20	26.131652344338914	23.553319262274485	25.395434443941905	24.919593949444696
21	26.631677471229708	24.638800944771095	24.08443891652847	24.645082667470728
22	26.634818332579524	23.85861098547666	23.868347655661086	25.63822302628273
23	24.998743655460075	24.460714106236495	24.62655158550681	25.91399065279662
24	25.689104980149757	24.29236393788633	24.800555304286647	25.21797577767727
25	25.565040956832004	23.555517865219358	24.81249057741595	26.06695060053269
26	25.614666566159105	24.4092039800995	24.66989547213428	25.306233981607118
27	25.879441177948642	23.543582592090054	24.445952057892356	26.13102417206895
28	24.529813055932458	24.396954620835217	25.42778531584502	25.645447007387308
29	24.54740187949143	24.84107241569928	25.57980300517614	25.031722699633146
30	24.016282225237447	25.707636062113675	25.48306447560179	24.79301723704709
31	25.226456103321777	24.74213528318006	24.769460776923463	25.2619478365747
32	24.09574601738781	24.49337906427459	25.537087290818633	25.87378762751897
33	24.401351826724962	23.782288054676116	25.821963415246994	25.99439670335193
34	25.329162269460774	23.77003869541183	25.34455249007488	25.556246545052513
35	24.98869289914066	23.74742449369315	25.704495200763855	25.559387406402333
36	23.935876174682143	24.05585707824514	26.192270968390368	25.815995778682343
37	24.976443539876374	22.385861098547664	26.203578069249712	26.43411729232625
38	23.801761395044977	22.28786622443339	27.824576611890045	26.08579576863159
39	23.926453590632697	21.82773003668526	28.320518619026082	25.92529775365596
40	23.28885873661993	21.923840393989646	28.855407306899846	25.931893562490576
41	21.99890698025026	22.533795668124025	29.97826523945927	25.48903211216644
42	22.801397055128398	21.71434494195688	29.607957686315896	25.876300316598822
43	20.986607367204382	22.01115633951455	29.905711342278508	27.096524951002564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	25.0
2	30.0
3	61.5
4	93.0
5	93.0
6	130.0
7	167.0
8	168.0
9	169.0
10	282.0
11	395.0
12	395.0
13	651.0
14	907.0
15	1641.0
16	2375.0
17	2230.5
18	2086.0
19	2086.0
20	2430.0
21	2774.0
22	2621.0
23	2468.0
24	2645.0
25	2822.0
26	2822.0
27	3112.0
28	3402.0
29	4654.5
30	5907.0
31	6316.0
32	6725.0
33	6725.0
34	7739.5
35	8754.0
36	9705.0
37	10656.0
38	12525.0
39	14394.0
40	14394.0
41	15924.0
42	17454.0
43	17445.5
44	17437.0
45	19000.5
46	20564.0
47	20564.0
48	22432.5
49	24301.0
50	25276.0
51	26251.0
52	26959.0
53	27667.0
54	27667.0
55	24750.5
56	21834.0
57	20774.0
58	19714.0
59	18340.0
60	16966.0
61	16966.0
62	15938.0
63	14910.0
64	13938.0
65	12966.0
66	11665.5
67	10365.0
68	10365.0
69	9044.5
70	7724.0
71	6766.5
72	5809.0
73	4695.5
74	3582.0
75	3582.0
76	2909.5
77	2237.0
78	1928.5
79	1620.0
80	1376.5
81	1133.0
82	1133.0
83	927.5
84	722.0
85	581.0
86	440.0
87	371.5
88	303.0
89	303.0
90	243.0
91	183.0
92	110.0
93	37.0
94	26.0
95	15.0
96	15.0
97	9.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	318384.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.22056622020527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.61169011434879	53.964667186356564
2	4.946398837567257	5.957498774985307
3	1.576565199775785	2.8482494704070644
4	0.8608941808406495	2.0737414010361483
5	0.505826518067483	1.5230579663609363
6	0.3677301433712285	1.3286950470031587
7	0.2602280142348315	1.0969754854506832
8	0.21217534899128063	1.0221855723379663
9	0.16650472453429288	0.9024307910814979
>10	1.2619720456213193	15.530578899223165
>50	0.1566616102995175	6.4298048286189164
>100	0.07178140672586587	6.45694013214533
>500	0.0010479037478228596	0.5111533920091591
>1k	5.239518739114298E-4	0.35402105298412134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1122	0.3524046434494196	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	869	0.27294085129906026	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	751	0.2358786873712247	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	397	0.12469219558771798	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	377	0.11841047288808483	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	353	0.11087240564852505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.1408613498165735E-4	0.0
5	0.0	0.0	0.0	3.1408613498165735E-4	0.0
6	0.0	0.0	0.0	3.1408613498165735E-4	0.0
7	0.0	0.0	0.0	3.1408613498165735E-4	0.0
8	0.0	0.0	0.0	3.1408613498165735E-4	0.0
9	0.0	0.0	0.0	3.1408613498165735E-4	0.0
10	0.0	0.0	0.0	3.1408613498165735E-4	0.0
11	0.0	0.0	0.0	3.1408613498165735E-4	0.0
12	0.0	0.0	0.0	3.1408613498165735E-4	0.0
13	0.0	0.0	0.0	3.1408613498165735E-4	0.0
14	0.0	0.0	0.0	3.1408613498165735E-4	0.0
15	0.0	0.0	0.0	6.281722699633147E-4	0.0
16	0.0	0.0	0.0	6.281722699633147E-4	0.0
17	0.0	0.0	0.0	9.422584049449722E-4	0.0
18	0.0	0.0	0.0	9.422584049449722E-4	0.0
19	0.0	3.1408613498165735E-4	0.0	9.422584049449722E-4	0.0
20	0.0	3.1408613498165735E-4	0.0	0.0018845168098899443	0.0
21	0.0	3.1408613498165735E-4	0.0	0.002512689079853259	0.0
22	0.0	3.1408613498165735E-4	0.0	0.002512689079853259	0.0
23	0.0	3.1408613498165735E-4	0.0	0.003454947484798231	0.0
24	0.0	3.1408613498165735E-4	0.0	0.00722398110457812	0.0
25	0.0	3.1408613498165735E-4	0.0	0.008166239509523091	0.0
26	0.0	3.1408613498165735E-4	0.0	0.012563445399266295	0.0
27	0.0	3.1408613498165735E-4	0.0	0.03800442233278054	0.0
28	0.0	3.1408613498165735E-4	0.0	0.1664656515402784	0.0
29	0.0	3.1408613498165735E-4	0.0	0.35711593547414444	0.0
30	0.0	3.1408613498165735E-4	0.0	0.588597416955626	0.0
31	0.0	3.1408613498165735E-4	0.0	1.309111010603548	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCCC	25	0.00549045	29.599998	31
GTGTAAA	40	0.0019281378	23.125	1
ATGGTCG	40	0.0019281378	23.125	36
ATACACA	105	4.0017767E-11	22.90476	37
TCTAGCG	50	2.69616E-4	22.199999	28
CTAGCGG	50	2.69616E-4	22.199999	29
GGTATCA	415	0.0	20.951807	1
TCGTTTA	45	0.0038198242	20.555557	30
TCAGGCG	45	0.0038198242	20.555557	4
CGTTTAT	45	0.0038198242	20.555557	31
CTTATAC	420	0.0	20.261904	37
AGACAGT	55	5.131875E-4	20.181818	6
TATACAC	120	2.3646862E-10	20.041666	36
GACAGTG	60	9.216834E-4	18.5	7
GTCCTAA	60	9.216834E-4	18.5	1
GCAGTCG	220	0.0	18.5	9
GCCTGTA	60	9.216834E-4	18.5	2
AGATAAC	50	0.0070239874	18.499998	5
ATCGTTT	50	0.0070239874	18.499998	29
TATACAA	50	0.0070239874	18.499998	5
>>END_MODULE