FastQCFastQC Report
Fri 10 Feb 2017
ERR1631517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937124
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29720.3171405278276941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20240.21597995569423042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20150.21501957051574822No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA9800.10457527499028944No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9480.10116057213346366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC450.003825288320.5555553
AGTCGGT7600.020.44736911
GGTATCA21150.020.2056751
CGAACTA555.1421206E-420.18181829
TAGTTCG555.1421206E-420.18181812
GGCAGTC8250.019.7333348
ATACACA6950.019.69784237
GCAGTCG8450.019.4852079
CAGTCGG8000.019.42500110
TTTAGGT609.2350814E-418.53
CTATACA609.2350814E-418.54
TATACAC11200.017.83928537
TAACCCG2650.017.4528328
GCGTATA852.7222464E-517.4117649
TAATACT1401.8681021E-917.1785724
TACTGTG1301.3920726E-817.0769237
GAACCGA650.001579639717.0769236
CTTATAC34850.016.88091737
GGGCAGT9650.016.8704667
TCGGTGA9550.016.4659713
Produced by FastQC (version 0.11.5)