##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631517.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	937124
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65051369936102	34.0	31.0	34.0	31.0	34.0
2	32.83972451884703	34.0	31.0	34.0	31.0	34.0
3	32.94300967641422	34.0	31.0	34.0	31.0	34.0
4	36.353731203127865	37.0	37.0	37.0	35.0	37.0
5	36.300065946448925	37.0	37.0	37.0	35.0	37.0
6	36.344839103469766	37.0	37.0	37.0	35.0	37.0
7	36.3365669857991	37.0	37.0	37.0	35.0	37.0
8	36.3198434785578	37.0	37.0	37.0	35.0	37.0
9	38.13934868811385	39.0	38.0	39.0	37.0	39.0
10	38.06530192375822	39.0	38.0	39.0	37.0	39.0
11	38.13925371669064	39.0	38.0	39.0	37.0	39.0
12	37.91809194941118	39.0	38.0	39.0	35.0	39.0
13	38.04962096798289	39.0	38.0	39.0	37.0	39.0
14	39.503267443796126	40.0	39.0	41.0	37.0	41.0
15	39.4925004588507	40.0	39.0	41.0	37.0	41.0
16	39.47162061797585	40.0	39.0	41.0	37.0	41.0
17	39.45161899599199	40.0	39.0	41.0	37.0	41.0
18	39.458587124009206	40.0	39.0	41.0	37.0	41.0
19	39.47469385054699	40.0	39.0	41.0	37.0	41.0
20	39.455397578121996	40.0	39.0	41.0	37.0	41.0
21	39.43509183416496	40.0	39.0	41.0	37.0	41.0
22	39.385515684157056	40.0	39.0	41.0	37.0	41.0
23	39.331552708072785	40.0	39.0	41.0	36.0	41.0
24	39.304550945232435	40.0	39.0	41.0	36.0	41.0
25	39.284318830805745	40.0	39.0	41.0	36.0	41.0
26	39.211532305223216	40.0	39.0	41.0	36.0	41.0
27	39.142994950508154	40.0	39.0	41.0	36.0	41.0
28	39.05683666195722	40.0	39.0	41.0	35.0	41.0
29	38.97375374016672	40.0	39.0	41.0	35.0	41.0
30	38.91124333599396	40.0	38.0	41.0	35.0	41.0
31	38.85132063632988	40.0	38.0	41.0	35.0	41.0
32	38.77368950106923	40.0	38.0	41.0	35.0	41.0
33	38.69162458756792	40.0	38.0	41.0	35.0	41.0
34	38.6412641229976	40.0	38.0	41.0	35.0	41.0
35	38.559862942364084	40.0	38.0	41.0	35.0	41.0
36	38.505910637226236	40.0	38.0	41.0	35.0	41.0
37	38.44625257703356	40.0	38.0	41.0	35.0	41.0
38	38.37444671142773	40.0	38.0	41.0	35.0	41.0
39	38.29881104314904	40.0	38.0	41.0	34.0	41.0
40	38.209875107243015	40.0	38.0	41.0	34.0	41.0
41	38.111572214562855	40.0	37.0	41.0	34.0	41.0
42	38.00442204019959	40.0	37.0	41.0	34.0	41.0
43	37.06235354126028	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	4.0
13	6.0
14	2.0
15	2.0
16	7.0
17	19.0
18	24.0
19	36.0
20	71.0
21	154.0
22	260.0
23	420.0
24	664.0
25	989.0
26	1551.0
27	2162.0
28	3185.0
29	4393.0
30	6259.0
31	8112.0
32	10728.0
33	14847.0
34	21705.0
35	32704.0
36	53612.0
37	104509.0
38	249411.0
39	421284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7181205475476	16.94023416324841	13.239870070556298	26.101775218647695
2	19.82106957030233	19.723750538882793	33.3821351283288	27.073044762486077
3	19.523350164972832	21.532689377286253	29.194749040681916	29.749211417059
4	14.593906462751994	16.16392281064192	33.42044382600381	35.82172690060227
5	16.004072033156767	34.79283424605495	32.47329062109176	16.729803099696518
6	34.13134227700923	33.942253106312506	16.590440539352315	15.335964077325947
7	30.865605832312482	29.689560826528826	19.83323444922977	19.61159889192892
8	29.38714620477119	31.911572001143924	18.874983460033036	19.826298334051845
9	28.046021657752867	14.451235909015242	18.171554671526927	39.33118776170496
10	18.779905327363295	25.012698426248818	29.790614689198016	26.41678155718987
11	37.24907269475544	21.665009112988248	19.74647965477354	21.339438537482767
12	24.188794652575325	25.038948954460672	26.56766873967586	24.204587653288144
13	31.64479834045441	18.10742228349717	24.167132631327338	26.08064674472108
14	24.143976677579488	19.094911665905475	23.661756608517123	33.09935504799792
15	27.122344534981497	25.53600163905737	20.841532177171857	26.500121648789275
16	26.381247305586026	24.660343775210112	22.858554470913134	26.099854448290728
17	24.72319564966856	25.716340633683483	23.663677378874088	25.896786337773868
18	25.6924377136857	23.356887669081146	25.10553566016877	25.84513895706438
19	26.110632104182585	24.69321029020706	24.544243878078035	24.651913727532325
20	26.775965613942233	22.998450578578716	24.5333595127219	25.69222429475715
21	28.249196477734	23.22552831855763	23.82310131850214	24.70217388520623
22	27.897055245623847	22.943281785548123	23.194369154989094	25.965293813838937
23	26.10358927954038	23.337039708725847	23.81168340582463	26.747687605909142
24	25.2452183489058	24.02841032776879	24.58095193378891	26.145419389536496
25	25.47272292674182	23.054366337859236	24.44852548862264	27.024385246776305
26	26.13698934185871	23.818192683145455	24.38151194505743	25.66330602993841
27	26.315194147199307	23.24452260319872	23.790875060290848	26.649408189311124
28	25.392157281213585	23.518018960137614	24.961798011789263	26.12802574685954
29	25.052074218566595	23.684912562264973	25.52276966548717	25.740243553681264
30	24.66898724181645	24.047191193481332	25.5993870608372	25.684434503865017
31	25.426731147638947	23.639561039947754	24.474989435763035	26.458718376650264
32	24.451940191479462	22.967398124474457	25.84695301795707	26.73370866608901
33	24.708256324669946	22.384017483278626	26.54152492092829	26.366201271123142
34	25.479338913526917	22.010854486706137	26.133254510609056	26.37655208915789
35	24.667066471459485	22.10486552473312	26.660079135738705	26.56798886806869
36	23.347817364617704	22.14221383722965	27.240792040327637	27.269176757825004
37	23.58673985513123	20.977800163052063	27.889692292588812	27.545767689227894
38	22.76753129788587	20.12732573277389	29.592988761359223	27.512154207981016
39	22.848096943414106	19.976758678680728	30.386053499857006	26.789090878048157
40	22.220005036686715	20.605277423265225	30.34027513968269	26.834442400365372
41	20.686696744507664	20.83075452128	31.866860735612363	26.615687998599974
42	20.562060090233523	21.267516358560872	31.346118549946432	26.824305001259173
43	19.204075447859623	21.137650940537217	31.5500403361775	28.108233275425665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	25.0
2	37.0
3	165.5
4	294.0
5	294.0
6	425.0
7	556.0
8	595.5
9	635.0
10	895.5
11	1156.0
12	1156.0
13	1882.0
14	2608.0
15	4179.0
16	5750.0
17	5184.5
18	4619.0
19	4619.0
20	5116.0
21	5613.0
22	4654.0
23	3695.0
24	4135.5
25	4576.0
26	4576.0
27	5564.5
28	6553.0
29	8296.0
30	10039.0
31	12356.5
32	14674.0
33	14674.0
34	18096.5
35	21519.0
36	25984.5
37	30450.0
38	35111.0
39	39772.0
40	39772.0
41	44155.0
42	48538.0
43	53725.0
44	58912.0
45	63628.0
46	68344.0
47	68344.0
48	73930.5
49	79517.0
50	79451.0
51	79385.0
52	77771.0
53	76157.0
54	76157.0
55	72068.0
56	67979.0
57	65375.5
58	62772.0
59	60866.5
60	58961.0
61	58961.0
62	54329.0
63	49697.0
64	45320.5
65	40944.0
66	36363.0
67	31782.0
68	31782.0
69	26999.0
70	22216.0
71	19013.0
72	15810.0
73	12341.0
74	8872.0
75	8872.0
76	7132.0
77	5392.0
78	4465.5
79	3539.0
80	3061.5
81	2584.0
82	2584.0
83	2022.5
84	1461.0
85	1157.5
86	854.0
87	672.5
88	491.0
89	491.0
90	372.5
91	254.0
92	158.5
93	63.0
94	47.0
95	31.0
96	31.0
97	19.5
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	937124.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.9031788268551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79176433281243	60.073544663369795
2	5.837090751121283	7.810398527016982
3	1.6133205300824454	3.238088157874275
4	0.7276129736865198	1.9471848358115618
5	0.41232697920808015	1.3792992812547573
6	0.28874354855126805	1.1590716758295723
7	0.19801824633492202	0.9273635101867871
8	0.13830750672169392	0.7402569484238362
9	0.10902312420331933	0.6564594217353396
>10	0.7447301909196624	9.620295429953238
>50	0.08139171270304492	3.7405748843046482
>100	0.05494679328337384	6.948822096874512
>500	0.0022427261887072946	1.0072340820338386
>1k	4.8058418329442027E-4	0.7514064853308409
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2972	0.3171405278276941	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2024	0.21597995569423042	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2015	0.21501957051574822	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	980	0.10457527499028944	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	948	0.10116057213346366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.0670946427580556E-4	0.0	1.0670946427580556E-4	0.0
4	0.0	1.0670946427580556E-4	0.0	1.0670946427580556E-4	0.0
5	0.0	1.0670946427580556E-4	0.0	1.0670946427580556E-4	0.0
6	0.0	1.0670946427580556E-4	0.0	1.0670946427580556E-4	0.0
7	0.0	1.0670946427580556E-4	0.0	2.134189285516111E-4	0.0
8	0.0	1.0670946427580556E-4	0.0	2.134189285516111E-4	0.0
9	0.0	1.0670946427580556E-4	0.0	2.134189285516111E-4	0.0
10	0.0	1.0670946427580556E-4	0.0	3.2012839282741663E-4	0.0
11	0.0	1.0670946427580556E-4	0.0	7.469662499306389E-4	0.0
12	0.0	1.0670946427580556E-4	0.0	9.603851784822499E-4	0.0
13	0.0	1.0670946427580556E-4	0.0	0.0013872230355854722	0.0
14	0.0	1.0670946427580556E-4	0.0	0.001707351428412889	0.0
15	0.0	1.0670946427580556E-4	0.0	0.0022408987497919163	0.0
16	0.0	1.0670946427580556E-4	0.0	0.004161669106756417	1.0670946427580556E-4
17	0.0	1.0670946427580556E-4	0.0	0.005975729999445111	1.0670946427580556E-4
18	0.0	1.0670946427580556E-4	0.0	0.0062958583922725275	1.0670946427580556E-4
19	0.0	1.0670946427580556E-4	0.0	0.007042824642203166	1.0670946427580556E-4
20	0.0	1.0670946427580556E-4	0.0	0.008109919284961221	1.0670946427580556E-4
21	0.0	1.0670946427580556E-4	0.0	0.009817270713374111	2.134189285516111E-4
22	0.0	1.0670946427580556E-4	0.0	0.013872230355854722	2.134189285516111E-4
23	0.0	1.0670946427580556E-4	0.0	0.019847960355299833	2.134189285516111E-4
24	0.0	1.0670946427580556E-4	0.0	0.033506771782602945	2.134189285516111E-4
25	0.0	1.0670946427580556E-4	0.0	0.039909339639151276	2.134189285516111E-4
26	0.0	1.0670946427580556E-4	0.0	0.04972661035252539	2.134189285516111E-4
27	0.0	1.0670946427580556E-4	0.0	0.13114593159496502	2.134189285516111E-4
28	0.0	1.0670946427580556E-4	0.0	0.5357882201288197	2.134189285516111E-4
29	0.0	1.0670946427580556E-4	0.0	1.1489408018575984	2.134189285516111E-4
30	0.0	1.0670946427580556E-4	0.0	2.0060312189208687	2.134189285516111E-4
31	0.0	1.0670946427580556E-4	0.0	3.7434747162595343	2.134189285516111E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATAC	45	0.0038252883	20.555555	3
AGTCGGT	760	0.0	20.447369	11
GGTATCA	2115	0.0	20.205675	1
CGAACTA	55	5.1421206E-4	20.181818	29
TAGTTCG	55	5.1421206E-4	20.181818	12
GGCAGTC	825	0.0	19.733334	8
ATACACA	695	0.0	19.697842	37
GCAGTCG	845	0.0	19.485207	9
CAGTCGG	800	0.0	19.425001	10
TTTAGGT	60	9.2350814E-4	18.5	3
CTATACA	60	9.2350814E-4	18.5	4
TATACAC	1120	0.0	17.839285	37
TAACCCG	265	0.0	17.45283	28
GCGTATA	85	2.7222464E-5	17.411764	9
TAATACT	140	1.8681021E-9	17.178572	4
TACTGTG	130	1.3920726E-8	17.076923	7
GAACCGA	65	0.0015796397	17.076923	6
CTTATAC	3485	0.0	16.880917	37
GGGCAGT	965	0.0	16.870466	7
TCGGTGA	955	0.0	16.46597	13
>>END_MODULE