FastQCFastQC Report
Fri 10 Feb 2017
ERR1631516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329548
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA39650.2982216512679497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34670.26076531272282005No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC31280.23526792564089452No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC29670.2231585471152602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28640.2154115534001029No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC27790.20901840324681772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25540.19209535872341577No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC21710.16328857626802493No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC20260.15238261424183255No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC19810.14899800533715218No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC18740.14095015749713435No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT18500.1391450327479715No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG18170.13666298621787254No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA17750.13350401790683752No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16120.121244212318773No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT16000.12034164994419157No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG15790.11876216578867406No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC15660.11778438988287748No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC14480.10890919319949337No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC14230.10702885491911537No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG14050.10567501135724322No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT13700.10304253776471403No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT13670.10281689717106866No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG13540.10183912126527211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC358.870129E-426.42857235
GGTATCA25650.022.6471731
GCTCTAT450.003826117620.5555571
TTAAGGT450.003826117620.5555574
TACACTT759.267562E-619.7333345
TATACAC8350.019.05389237
AGTTTAG500.007035446418.59
CGTCTAA902.1527321E-618.51
ATACGCA609.237852E-418.56
ATACCGT2300.018.56
TACCGTC2300.018.57
CTTATAC29900.018.19063637
GGCAGTC9550.017.6282738
GCAGTCG9600.017.5364599
TAAGATA852.7236354E-517.4117644
TAATGGT852.7236354E-517.41176416
TTGTATA752.0675734E-417.2666662
AGTGGCC650.001580109817.07692310
CTTACCG650.001580109817.0769239
CAGATAC2500.017.023
Produced by FastQC (version 0.11.5)