##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631516.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1329548
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75791547202508	34.0	31.0	34.0	31.0	34.0
2	32.94707524662517	34.0	33.0	34.0	31.0	34.0
3	33.05005310075304	34.0	33.0	34.0	31.0	34.0
4	36.40505344673528	37.0	37.0	37.0	35.0	37.0
5	36.38460740040976	37.0	37.0	37.0	35.0	37.0
6	36.440321823657364	37.0	37.0	37.0	35.0	37.0
7	36.43222433488674	37.0	37.0	37.0	35.0	37.0
8	36.41769684133254	37.0	37.0	37.0	35.0	37.0
9	38.26221768601059	39.0	39.0	39.0	37.0	39.0
10	38.21192390195766	39.0	39.0	39.0	37.0	39.0
11	38.276182582351296	39.0	39.0	39.0	37.0	39.0
12	38.06714387145105	39.0	39.0	39.0	37.0	39.0
13	38.18853625442632	39.0	39.0	39.0	37.0	39.0
14	39.677037609774146	41.0	40.0	41.0	37.0	41.0
15	39.67461573406902	41.0	40.0	41.0	37.0	41.0
16	39.643734562422715	41.0	39.0	41.0	37.0	41.0
17	39.62728235460472	41.0	39.0	41.0	37.0	41.0
18	39.62412037775244	41.0	40.0	41.0	37.0	41.0
19	39.64219042862687	41.0	40.0	41.0	37.0	41.0
20	39.62007765044962	41.0	40.0	41.0	37.0	41.0
21	39.59220878072849	41.0	40.0	41.0	37.0	41.0
22	39.53998200892333	41.0	39.0	41.0	37.0	41.0
23	39.4578413114833	41.0	39.0	41.0	37.0	41.0
24	39.43511779943259	41.0	39.0	41.0	37.0	41.0
25	39.4039651069386	41.0	39.0	41.0	36.0	41.0
26	39.33764331938373	41.0	39.0	41.0	36.0	41.0
27	39.25813283913029	41.0	39.0	41.0	36.0	41.0
28	39.13686531061684	41.0	39.0	41.0	35.0	41.0
29	39.06370285239796	40.0	39.0	41.0	35.0	41.0
30	38.98647059000503	40.0	39.0	41.0	35.0	41.0
31	38.92147857768204	40.0	39.0	41.0	35.0	41.0
32	38.83844434349117	40.0	38.0	41.0	35.0	41.0
33	38.72512613309185	40.0	38.0	41.0	35.0	41.0
34	38.68599930201843	40.0	38.0	41.0	35.0	41.0
35	38.56044084154916	40.0	38.0	41.0	35.0	41.0
36	38.50921967465635	40.0	38.0	41.0	35.0	41.0
37	38.43299151290514	40.0	38.0	41.0	35.0	41.0
38	38.33189550132827	40.0	38.0	41.0	34.0	41.0
39	38.225723328529696	40.0	38.0	41.0	34.0	41.0
40	38.117123262943494	40.0	37.0	41.0	34.0	41.0
41	38.00999437402786	40.0	37.0	41.0	34.0	41.0
42	37.87537042664123	40.0	37.0	41.0	34.0	41.0
43	36.952768910938154	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	4.0
14	2.0
15	5.0
16	5.0
17	11.0
18	34.0
19	61.0
20	103.0
21	225.0
22	380.0
23	653.0
24	1080.0
25	1617.0
26	2256.0
27	3260.0
28	4578.0
29	6302.0
30	8465.0
31	11015.0
32	14673.0
33	19706.0
34	30057.0
35	44361.0
36	69950.0
37	135084.0
38	318427.0
39	657231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88626811517899	18.061250891280345	12.553213573334698	25.49926742020596
2	21.92812895811208	19.160647077051753	31.655344523101085	27.255879441735086
3	20.73050390057373	21.523931441362027	28.606940102952283	29.138624555111964
4	14.591048988077151	16.58887080421316	34.1461910363522	34.67388917135749
5	15.626513672315703	34.996480006739134	32.73864501319245	16.63836130775271
6	32.67539043344054	34.70623098977998	16.714778255467273	15.903600321312206
7	30.26359334149651	29.336436142207727	20.176857097299234	20.22311341899653
8	29.29476784591455	30.24824978112862	18.540737152776735	21.91624522018009
9	27.47745850469483	14.381955371299119	19.524605354601714	38.61598076940434
10	20.687857828374757	23.529048970025904	29.669030377240986	26.114062824358353
11	37.04672565413208	22.30953677490395	19.395915002692647	21.24782256827132
12	24.44191559838381	25.499342633737182	24.710653545415433	25.348088222463574
13	31.246408553884482	18.358494766642497	24.336391014089	26.058705665384025
14	24.140083697617538	20.093294864119233	22.966903037724098	32.79971840053913
15	28.082852217445325	25.635103057580473	20.51952994551532	25.762514779458883
16	27.38757833489276	24.2488424637546	22.606028514953955	25.757550686398684
17	26.14369695565711	25.09266307045703	22.663190798677448	26.100449175208418
18	25.88955043368122	22.332251261330917	24.6920758032053	27.08612250178256
19	25.812606991248156	24.584294812974033	24.202360501463655	25.400737694314156
20	26.28630181084098	22.659730976241548	24.637320352480693	26.41664686043678
21	27.706258066651223	23.453985865873214	24.098490614855574	24.741265452619988
22	28.98466245671461	22.261099260801416	22.98367565518507	25.770562627298904
23	26.571135453552635	22.50095521184643	24.251023656159838	26.676885678441092
24	24.775938890510158	24.039974487570213	23.889246571015114	27.29484005090452
25	26.102329513488794	22.730732549708623	24.40175157271494	26.765186364087647
26	25.979505817014502	24.210408349303673	24.149334961956995	25.660750871724826
27	26.92343563376426	22.804742664424303	24.07036075418112	26.201460947630324
28	25.272272982998732	23.169979572004923	25.343124129403378	26.214623315592966
29	25.001128202968225	23.234211927662635	25.781017308137805	25.983642561231335
30	24.19641863249766	24.609416132399883	25.836976175361855	25.357189059740605
31	26.175060998173816	23.84983468065839	23.919256769969945	26.055847551197854
32	25.033545234921945	22.91079374343762	25.14358263108966	26.912078390550775
33	23.868412422868523	22.890786944134398	25.663383345317353	27.577417287679722
34	26.100449175208418	21.850207739773218	25.6556363516022	26.393706733416167
35	25.225189312458067	22.317960690400046	26.61084819803422	25.846001799107665
36	23.971530174164453	22.945091113671715	26.05471934822962	27.02865936393421
37	25.15997918089456	20.670032221476774	27.398634723981385	26.771353873647286
38	23.471661045708768	20.296747466056132	28.282168075165394	27.949423413069706
39	23.703920430101057	20.676801439286134	29.14464163760917	26.47463649300364
40	22.976981650906925	20.62520495687256	29.473174341956813	26.9246390502637
41	21.656382469831854	20.92342660814051	30.792720533594874	26.627470388432762
42	21.987698074834455	21.524081868424457	30.269685637524933	26.218534419216155
43	19.94850881653013	20.767809812056427	30.84168454241592	28.441996828997524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	32.5
2	52.0
3	198.5
4	345.0
5	345.0
6	498.0
7	651.0
8	678.0
9	705.0
10	1057.5
11	1410.0
12	1410.0
13	2459.5
14	3509.0
15	5205.5
16	6902.0
17	6217.0
18	5532.0
19	5532.0
20	5849.5
21	6167.0
22	4524.5
23	2882.0
24	2839.5
25	2797.0
26	2797.0
27	3681.0
28	4565.0
29	7579.5
30	10594.0
31	14565.0
32	18536.0
33	18536.0
34	24525.0
35	30514.0
36	37464.0
37	44414.0
38	50764.0
39	57114.0
40	57114.0
41	66903.5
42	76693.0
43	85078.5
44	93464.0
45	98770.5
46	104077.0
47	104077.0
48	111022.5
49	117968.0
50	115319.0
51	112670.0
52	112424.5
53	112179.0
54	112179.0
55	102026.0
56	91873.0
57	83287.5
58	74702.0
59	73398.0
60	72094.0
61	72094.0
62	70975.0
63	69856.0
64	64398.5
65	58941.0
66	55120.5
67	51300.0
68	51300.0
69	42640.5
70	33981.0
71	28716.5
72	23452.0
73	17458.5
74	11465.0
75	11465.0
76	9245.5
77	7026.0
78	6401.5
79	5777.0
80	5433.0
81	5089.0
82	5089.0
83	4505.5
84	3922.0
85	3236.5
86	2551.0
87	2148.5
88	1746.0
89	1746.0
90	1518.0
91	1290.0
92	874.5
93	459.0
94	319.5
95	180.0
96	180.0
97	132.0
98	84.0
99	45.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1329548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.236328577655108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69129974479337	22.714083669638715
2	8.931499463610717	5.222485060185465
3	3.3814752341078473	2.965857630647408
4	1.7986083987399089	2.1033882451236035
5	1.2113546537621798	1.7707781340731361
6	0.8776672051342703	1.5395860074682652
7	0.6756039580285845	1.3826525513701604
8	0.551745484334609	1.290480981699527
9	0.40484309824408504	1.065251325839398
>10	3.5629508636281524	21.410661470932002
>50	0.5337875678463585	10.969602038014076
>100	0.3488622605989213	18.561563378679516
>500	0.02123734622243206	4.273168937742557
>1k	0.00906472094859905	4.730440568586149
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3965	0.2982216512679497	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3467	0.26076531272282005	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3128	0.23526792564089452	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2967	0.2231585471152602	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2864	0.2154115534001029	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2779	0.20901840324681772	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2554	0.19209535872341577	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2171	0.16328857626802493	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	2026	0.15238261424183255	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1981	0.14899800533715218	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1874	0.14095015749713435	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1850	0.1391450327479715	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	1817	0.13666298621787254	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1775	0.13350401790683752	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1612	0.121244212318773	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	1600	0.12034164994419157	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	1579	0.11876216578867406	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	1566	0.11778438988287748	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	1448	0.10890919319949337	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	1423	0.10702885491911537	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1405	0.10567501135724322	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	1370	0.10304253776471403	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	1367	0.10281689717106866	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1354	0.10183912126527211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	7.521353121511973E-5
6	0.0	0.0	0.0	0.0	7.521353121511973E-5
7	0.0	0.0	0.0	0.0	7.521353121511973E-5
8	0.0	0.0	0.0	0.0	7.521353121511973E-5
9	0.0	0.0	0.0	3.7606765607559864E-4	7.521353121511973E-5
10	0.0	0.0	0.0	3.7606765607559864E-4	7.521353121511973E-5
11	0.0	0.0	0.0	4.5128118729071835E-4	7.521353121511973E-5
12	0.0	0.0	0.0	4.5128118729071835E-4	7.521353121511973E-5
13	0.0	0.0	0.0	4.5128118729071835E-4	7.521353121511973E-5
14	0.0	0.0	0.0	6.017082497209578E-4	7.521353121511973E-5
15	0.0	0.0	0.0	8.27348843366317E-4	7.521353121511973E-5
16	0.0	0.0	0.0	0.0014290570930872747	7.521353121511973E-5
17	0.0	0.0	0.0	0.0018051247491628734	7.521353121511973E-5
18	0.0	0.0	0.0	0.001880338280377993	7.521353121511973E-5
19	0.0	0.0	0.0	0.0022564059364535917	7.521353121511973E-5
20	0.0	0.0	0.0	0.0024068329988838314	7.521353121511973E-5
21	0.0	0.0	0.0	0.0029333277173896693	7.521353121511973E-5
22	0.0	0.0	0.0	0.003911103623186226	7.521353121511973E-5
23	0.0	1.5042706243023946E-4	0.0	0.006844431340575895	1.5042706243023946E-4
24	0.0	1.5042706243023946E-4	0.0	0.015193133305454184	1.5042706243023946E-4
25	0.0	1.5042706243023946E-4	0.0	0.020909361677803284	1.5042706243023946E-4
26	0.0	1.5042706243023946E-4	0.0	0.0385093279821413	1.5042706243023946E-4
27	0.0	1.5042706243023946E-4	0.0	0.0995075017976034	1.5042706243023946E-4
28	0.0	1.5042706243023946E-4	0.0	0.3107823109808747	1.5042706243023946E-4
29	0.0	1.5042706243023946E-4	0.0	0.6046415774383475	1.5042706243023946E-4
30	0.0	1.5042706243023946E-4	0.0	1.035991178957059	1.5042706243023946E-4
31	0.0	1.5042706243023946E-4	0.0	2.06378408301167	1.5042706243023946E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGC	35	8.870129E-4	26.428572	35
GGTATCA	2565	0.0	22.647173	1
GCTCTAT	45	0.0038261176	20.555557	1
TTAAGGT	45	0.0038261176	20.555557	4
TACACTT	75	9.267562E-6	19.733334	5
TATACAC	835	0.0	19.053892	37
AGTTTAG	50	0.0070354464	18.5	9
CGTCTAA	90	2.1527321E-6	18.5	1
ATACGCA	60	9.237852E-4	18.5	6
ATACCGT	230	0.0	18.5	6
TACCGTC	230	0.0	18.5	7
CTTATAC	2990	0.0	18.190636	37
GGCAGTC	955	0.0	17.628273	8
GCAGTCG	960	0.0	17.536459	9
TAAGATA	85	2.7236354E-5	17.411764	4
TAATGGT	85	2.7236354E-5	17.411764	16
TTGTATA	75	2.0675734E-4	17.266666	2
AGTGGCC	65	0.0015801098	17.076923	10
CTTACCG	65	0.0015801098	17.076923	9
CAGATAC	250	0.0	17.02	3
>>END_MODULE