FastQCFastQC Report
Fri 10 Feb 2017
ERR1631515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290447
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49660.21681357394429998No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36290.15844068865160382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35390.154511324645364No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31580.1378770170189487No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC25460.11115734177651786No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC25340.11063342657568588No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24910.10875606377270464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33850.021.2599721
ATACACA14600.021.1609637
AACTCGC1254.129106E-1019.2422
ACTGATC2900.018.58
TACACAT5550.017.66666637
GTCTAGA3650.017.2328761
TATACAC24500.017.06530637
CTTATAC70400.016.71306837
GCCGTAT2250.016.4444458
AGTCGGT6200.016.41129111
CTAGACA3500.016.3857144
GTTCTAG3500.015.8571421
TCTAGAC3150.015.8571423
GCAGTCG6550.015.8167949
TTAGGCC3300.015.696974
CTAGAAC4450.015.3820223
TAGAACT7800.014.7051294
TAGGCCG2900.014.6724145
CCGTATG2650.014.66037759
CTCTATG11350.014.5066081
Produced by FastQC (version 0.11.5)