FastQCFastQC Report
Fri 10 Feb 2017
ERR1631514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341657
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.16361438518748336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5120.1498578984185894No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4320.1264426017906848No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4000.11707648313952297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3990.11678379193167415No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3620.1059542172412683No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3470.10156384912353618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC754.6020432E-1027.1333333
CTAGACA951.0913936E-1125.315794
GTTCTAG802.7115675E-823.1250021
CTTAGGG400.001928424923.1250023
GCACCCT502.6967202E-422.19999924
AACAGAT502.6967202E-422.1999997
GGTATCA3250.021.630771
TATACAC1600.020.812537
TTTCGGA1101.7425918E-920.18181817
ACCTTGA555.1329326E-420.1818181
CTTATAC5250.019.73333437
TTCGGAA1153.0286174E-919.30434618
GCTCTAG609.218715E-418.51
GAGCGAA902.1432224E-618.516
ACATGTA701.2160174E-418.58
ATCCGCC500.007025014618.49999823
GGGGTGT500.007025014618.4999986
GTAGACC500.007025014618.4999984
CTAAACA500.007025014618.4999984
GCTCGTA500.007025014618.4999989
Produced by FastQC (version 0.11.5)